Interaction of Monocyte-Derived Dendritic Cells with Ara h 2 from Raw and Roasted Peanuts

Authors: NOVAK, NATALLJA - University Of Bonn; Maleki, Soheila; CUADRADO, CARMEN - University Of Coruna; CRESPO, JESUS - Instituto De Investigacion Y Formacion Agraria Y Pesquera; CABANILLAS, BEATRIZ - University Of Bonn

Submitted to: Foods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/16/2020
Publication Date: 7/2/2020
Citation: Novak, N., Maleki, S.J., Cuadrado, C., Crespo, J.F., Cabanillas, B. 2020. Interaction of Monocyte-Derived Dendritic Cells with Ara h 2 from Raw and Roasted Peanuts. Foods. 9(7):863. https://dx.doi.org/10.3390/foods9070863.
DOI: https://doi.org/10.3390/foods9070863

Interpretive Summary: Specific type of immune cells, called dendritic cells have an essential function in the induction of allergy, tolerance and other immune responses. However, the role of dendritic cells in the initiation of food allergy and in the differential sensitization potential of certain food proteins is still largely unknown. The study of the role of human dendritic cells (MDDC) interaction with food proteins could shed light on the higher or lower allergenic potential of certain food allergens. In the present study, we have purified a peanut allergen with high allergic potency, Ara h 2, from raw and roasted peanut and analyzed its differential recognition and internalization by human MDDCs. Our results showed that the uptake and internalization of raw-Ara h 2 and roast-Ara h 2 by MDDCs was both time and dose-dependent, with a maximum in allergen internalization at 2 hours of allergen incubation. Our study also demonstrated that although the mannose receptor had a greater implication in the internalization of Ara h 2 from roasted peanut, this receptor is also important in the internalization of Ara h 2 from raw peanut, as opposed to other allergens such as raw Ara h 3.

Technical Abstract: Dendritic cells have an essential function in the induction of allergy, tolerance and other immune responses. However, the role of dendritic cells in the initiation of food allergy and in the differential sensitization potential of certain food proteins is still largely unknown. The study of the role of human monocyte-derived dendritic cells (MDDC) interaction with food proteins could shed light on the higher or lower allergenic potential of certain food allergens. In the present study, we have purified a peanut allergen with high allergic potency, Ara h 2, from raw and roasted peanut and analyzed its differential recognition and internalization by human MDDCs. Our results showed that the uptake and internalization of raw-Ara h 2 and roast-Ara h 2 by MDDCs was both time and dose-dependent, with a maximum in allergen internalization at 2 hours of allergen incubation. Our study also demonstrated that although the mannose receptor had a greater implication in the internalization of Ara h 2 from roasted peanut, this receptor is also important in the internalization of Ara h 2 from raw peanut, as opposed to other allergens such as raw Ara h 3.