Interaction of monocyte-dervied dendritic cells with Ara h 2 from raw and roasted peanut

Authors: NOVAK, NATALIJA - University Of Bonn; Maleki, Soheila; CUADRADO, CARMEN - National Research Institute For Food And Nutrition (INRAN); CRESPO, JESUS - Research Institute Of Horticulture; CABANILLAS, BEATRIZ - Research Institute Of Horticulture

Submitted to: Foods
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/16/2020
Publication Date: 7/2/2020
Citation: Novak, N., Maleki, S.J., Cuadrado, C., Crespo, J.F., Cabanillas, B. 2020. Interaction of monocyte-dervied dendritic cells with Ara h 2 from raw and roasted peanut. Foods. 9:863. http://dx.doi.org/10.3390/foods9070863.
DOI: https://doi.org/10.3390/foods9070863

Interpretive Summary: Ara h 2 is a relevant allergen from peanut linked to severe allergic reactions. The interaction of Ara h 2 with components of certain immune cells, referred to as dendritic cells, has not been investigated and could be key to understand the high potency of this allergen. In this study we aimed to analyze such interaction and the possible involved mechanism. Ara h 2 was purified from two forms of peanut, raw and roasted, and labeled with a fluorescent dye. Human monocyte-derived dendritic cells (MDDCs) were obtained and experiments of Ara h 2 internalization by MDDCs were carried out. The role of mannose receptor (a receptor on the surface of the cells) in the internalization of Ara h 2 from raw and roasted peanut was also investigated. Results showed that Ara h 2 internalization by MDDCs was both time and dose dependent. There were no significant differences in the internalization of Ara h 2 from roasted peanut versus raw peanut. Mannose receptor in MDDCs had a greater implication in the internalization of Ara h 2 from roasted peanut, however, this receptor was also important in the internalization of Ara h 2 from raw peanut, as opposed to other allergens such as raw Ara h 3.

Technical Abstract: Ara h 2 is a relevant allergen from peanut linked to severe allergic reactions. The interaction of Ara h 2 with components of the sensitization phase of food allergy, such as dendritic cells, has not been investigated and could be key to understand the allergenic potential of this allergen. In this study we aimed to analyze such interaction and the possible involved mechanism. Ara h 2 was purified from two forms of peanut, raw and roasted, and labeled with a fluorescent dye. Human monocyte-derived dendritic cells (MDDCs) were obtained and experiments of Ara h 2 internalization by MDDCs were carried out. The role of mannose receptor in the internalization of Ara h 2 from raw and roasted peanut was also investigated. Results showed that Ara h 2 internalization by MDDCs was both time and dose dependent. There were no significant differences in the internalization of Ara h 2 from roasted peanut with respect to raw peanut. Mannose receptor in MDDCs had a greater implication in the internalization of Ara h 2 from roasted peanut, however, this receptor was also important in the internalization of Ara h 2 from raw peanut, as opposed to other allergens such as raw Ara h 3.