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ARS Home » Pacific West Area » Pullman, Washington » WHGQ » Research » Publications at this Location » Publication #379483
Research Project: Biology, Ecology, and Genomics of Pathogenic and Beneficial Microorganisms of Wheat, Barley, and Biofuel Brassicas

Location: Wheat Health, Genetics, and Quality Research

Title: Plant-parasitic nematode communities associated with wheat-growing areas in central, eastern, and south-eastern Kazakhstan

Author
item IMREN, MUSTAFA - Bolu Abant Izzet Baysal University
item OZER, GOKSEL - Bolu Abant Izzet Baysal University
item Paulitz, Timothy
item MORGOUNOV, ALEXEY - International Maize & Wheat Improvement Center (CIMMYT)
item DABABAT, ABDELFATTAH - International Maize & Wheat Improvement Center (CIMMYT)

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/21/2021
Publication Date: 6/10/2021
Citation: Imren, M., Ozer, G., Paulitz, T.C., Morgounov, A., Dababat, A.A. 2021. Plant-parasitic nematode communities associated with wheat-growing areas in central, eastern, and south-eastern Kazakhstan. Plant Disease. https://doi.org/10.1094/PDIS-11-20-2424-SR.
DOI: https://doi.org/10.1094/PDIS-11-20-2424-SR

Interpretive Summary: A survey of plant pathogenic nematodes was conducted on wheat in Kazakhstan in 2019. Nematodes were identified with morphological and molecular methods. Root lesion nematodes (Pratylenchus neglectus and thornii) were predominant in most areas, while the all the cyst nematodes were identifed as Heterodera filipjevi. Many were present at population densities that could cause yield declines. The populations of Pratylenchus uniform based on DNA, but there was some intraspecific variation in H. filipjevi.

Technical Abstract: Kazakhstan is one of the biggest wheat producers, however, its wheat production is still far below the average international wheat production standard due to some biotic and abiotic stressors. Plant-parasitic nematodes are devastating biotic factors for cereal production systems worldwide. A comprehensive survey was conducted in 2019 to identify plant-parasitic nematodes intimately associated with wheat in different locations of central, eastern, and south-eastern Kazakhstan. The results revealed the presence of cyst and root-lesion nematodes predominantly in wheat-growing regions. Thirty-three root-lesion and twenty-seven cyst nematode populations were obtained from 77 localities. The two genera occurred in separate or in mixed populations in the fields. The root-lesion populations were identified as Pratylenchus neglectus and P. thornei while all cyst nematodes were identified as Heterodera filipjevi. The identification of nematodes was firstly performed based on morphological and morphometric features and confirmed by BLAST and phylogenetic analyses based on the internal transcribed spacer and the D2-D3 expansion located in the 28S gene of ribosomal DNA for cyst and root-lesion nematodes populations, respectively. Pratylenchus neglectus and P. thornei populations from Kazakhstan showed a high similarity with the American, European, and Asian populations. Heterodera filipjevi populations formed a well-supported cluster with the corresponding populations from different countries and showed a slightly intraspecific polymorphism. Kazakhstan populations of Heterodera filipjevi are probably from multiple introductions in Kazakhstan due to the divergence among them. The results of this study are of great importance for breeding programs and give awareness to extension services which will enable extension advisors to them to develop the right measures to control these devastating nematodes in cereal cropping areas in Kazakhstan. Keywords: Heterodera filipjevi