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ARS Home » Pacific West Area » Pullman, Washington » Grain Legume Genetics Physiology Research » Research » Publications at this Location » Publication #380123
Research Project: Enhanced Agronomic Performance and Disease Resistance in Edible Legumes

Location: Grain Legume Genetics Physiology Research

Title: A common bean truncated CRINKLY4 kinase controls gene-for-gene resistance to the fungal pathogen Colletotrichum lindemuthianum

Author
item RICHARD, MANON - Institute Of Plant Sciences Paris-Saclay
item GRATIAS, ARIANE - Institute Of Plant Sciences Paris-Saclay
item ALVAREZ DIAZ, JUAN - Institute Of Plant Sciences Paris-Saclay
item THAREAU, VINCENT - Institute Of Plant Sciences Paris-Saclay
item PFLIEGER, STEPANIE - Institute Of Plant Sciences Paris-Saclay
item MEZIADI, CHOUAIB - Institute Of Plant Sciences Paris-Saclay
item BLANCHET, SOPHIE - Institute Of Plant Sciences Paris-Saclay
item Miklas, Phillip - Phil
item MARANDE, WILLIAM - Institut National De La Recherche Agronomique (INRA)
item BITOCCHI, ELENA - Polytechnic University Of Marche
item PAPA, ROBERTO - Polytechnic University Of Marche
item GEFFROY, VALERIE - Institute Of Plant Sciences Paris-Saclay

Submitted to: Journal of Experimental Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/5/2021
Publication Date: 5/4/2021
Citation: Richard, M., Gratias, A., Alvarez Diaz, J., Thareau, V., Pflieger, S., Meziadi, C., Blanchet, S., Miklas, P.N., Marande, W., Bitocchi, E., Papa, R., Geffroy, V. 2021. A common bean truncated CRINKLY4 kinase controls gene-for-gene resistance to the fungal pathogen Colletotrichum lindemuthianum. Journal of Experimental Biology. 72(10):3569-3581. https://doi.org/10.1093/jxb/erab082.
DOI: https://doi.org/10.1093/jxb/erab082

Interpretive Summary: Bean anthracnose is a fungal disease of common bean that causes substantial yield and quality loss in production fields in the Northern Plains and Great Lakes production regions. Genetic resistance in the bean is the best way to control this disease. However, genetic resistance is complex and not well understood. This study identifies and characterizes a gene KTR2/3 that provides resistance to strain 100 of the pathogen which overcomes most known resistance genes. The gene is expressed after infection by this strain and is thought to act as a decoy for pathogen effectors which results in resistance. These results greatly enhance our understanding of the bean host x anthracnose pathogen interaction, and will faciliate deployment of resistance to combat this problematic disease.

Technical Abstract: Identifying the molecular basis of resistance is critical to promote chemical free cropping system. In plants, NLR constitute the largest family of disease resistance (R) genes but can be rapidly overcome prompting to the research of alternative source of resistance. Anthracnose, caused by the fungus Colletotrichum lindemuthianum, is one of the most important diseases of common bean. This study aimed to identify the molecular basis of Co-x, an anthracnose R-gene conferring total resistance to the extremely virulent C. lindemuthianum strain 100. To that end, we sequenced the Co-x 58kb target region in the resistant JaloEEP558 (Co-x) and identified KTR2/3, an additional gene encoding a truncated and chimeric CRINKLY4 kinase, located within a CRINKLY4 kinase cluster. KTR2/3 presence is strictly correlated with resistance to strain 100 in a diversity panel of beans. Furthermore, KTR2/3 expression is upregulated 24 hours post-inoculation and its transient expression using Agrobacterium-transformation in susceptible genotype increases resistance to strain 100. Altogether, our results provide molecular evidence that Co-x encodes a truncated and chimeric CRINKLY4 kinase probably resulting from an unequal recombination event that occurred recently in the Andean domesticated gene pool. This atypical R-gene might act as a decoy involved in indirect recognition of a fungal effector.