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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Exotic & Emerging Avian Viral Diseases Research » Research » Publications at this Location » Publication #380779
Research Project: Intervention Strategies to Predict, Prevent and Control Disease Outbreaks Caused by Emerging Strains of Virulent Newcastle Disease Viruses

Location: Exotic & Emerging Avian Viral Diseases Research

Title: Whole-genome sequence of avian Coronavirus from a preserved 15-year-old sample confirms evidence of GA08-like strain circulation 4 years prior to its first reported outbreak

Author
item GORAICHUK, IRYNA - Consultant
item DAVIS, JAMES - Georgia Poultry Laboratory Network
item KULKARNI, ARUN - Georgia Poultry Laboratory Network
item Afonso, Claudio
item Suarez, David

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/15/2021
Publication Date: 4/15/2021
Citation: Goraichuk, I.V., Davis, J.F., Kulkarni, A.B., Afonso, C.L., Suarez, D.L. 2021. Whole-genome sequence of avian Coronavirus from a preserved 15-year-old sample confirms evidence of GA08-like strain circulation 4 years prior to its first reported outbreak. Microbiology Resource Announcements. 10(15):e01460-20. https://doi.org/10.1128/MRA.01460-20.
DOI: https://doi.org/10.1128/MRA.01460-20

Interpretive Summary: Through the use of next generation sequencing methods, we were able to sequence several viruses from clinical samples without the need of growing the viruses in the laboratory. The complete genome of an infectious bronchitis virus (IBV) , an avian coronavirus, was determined from a chicken sample. This virus was closely related to the GA08 strain of IBV that has become an important pathogen of chickens. This sample was collected more than 4 years before the characterization of the GA08 strain which shows it circulated in Georgia earlier than was previously known.

Technical Abstract: Here, we report the complete genome sequence of an Avian coronavirus strain GA08-like isolate from a fecal sample from a broiler chicken collected in Georgia in 2004. The viral genome in this 15-year-old sample provides evidence for the circulation of the GA08-like strain at least 4'years before its first report in 2008.