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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » Molecular Plant Pathology Laboratory » Research » Publications at this Location » Publication #381981
Research Project: Emerging Biotechnologies for Developing Improved Pest and Pathogen Resistant Sugar Beet

Location: Molecular Plant Pathology Laboratory

Title: The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance

Author
item Klink, Vincent
item DARWISH, OMAR - Texas Woman'S University
item ALKHAROUF, NADIM - Towson University
item LAWRENCE, KATHERINE - Auburn University

Submitted to: Journal of Plant Interactions
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/4/2021
Publication Date: 6/29/2021
Citation: Klink, V.P., Darwish, O., Alkharouf, N.W., Lawrence, K.S. 2021. The impact of pRAP vectors on plant genetic transformation and pathogenesis studies including an analysis of BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1)-mediated resistance. Journal of Plant Interactions. 16(1):270-283. https://doi.org/10.1080/17429145.2021.1940328.
DOI: https://doi.org/10.1080/17429145.2021.1940328

Interpretive Summary: Genetic transformation in crop plants can be problematic, slow and inefficient, especially in cases where pathogens can interfere with the expression of the gene(s) of interest. Plasmid sequences have been generated and their functionality analyzed to allow for efficient and effective genetic engineering. The results of the analysis are that plasmid sequences have been generated for the pRAP15 gene expression and pRAP17 gene suppression plasmids. Associated analyses reveal the efficiency that these plasmids function at. The experiments will impact scientists and groups aimed at crop improvement in ways that are beneficial to stakeholders and society.

Technical Abstract: Crop improvement can be facilitated through efficient gene transfer, leading to pRAP plasmid development. Comparative hairy root transformation results from 24 previously published articles examining 29,756 roots show a 70% transformation efficiency.Average gene overexpression was 11.24-fold and -3.84-fold in RNAi roots. New studies show Glycine max BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) overexpression leads to a 67% decrease in Heterodera glycines parasitism while BAK1-1 RNAi led to a 4.8-fold increase in parasitism. The results show pathogen associated molecular pattern triggered immunity (PTI) functions in the G. max-H. glycines pathosystem during defense. Consequently, the pRAP vectors have applicability for studying basic biology and defense in other agricultural plants including Manihot esculenta(cassava), Zea mays (maize), Oryza sativa(rice), Triticum aestivum (wheat), Sorghum bicolor (sorghum), Brassica rapa (rape seed), Solanum tuberosum (potato), Solanum lycopersicum (tomato), Elaes guineensis (oil palm), Saccharum officinalis (sugarcane) and Beta vulgaris (sugar beet) since each have BAK1 homologs.