Skip to main content
ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #389118
Research Project: Gene Discovery and Crop Design for Current and New Rice Management Practices and Market Opportunities

Location: Dale Bumpers National Rice Research Center

Title: Deciphering the role of ATP2-Binding Cassette (ABC) transporter candidate genes in rice sheath blight resistance

Author
item SINGH, PRATIBHA - Australian Centre For International Agricultural Research
item OH, YOUNGJAE - University Of Florida
item LEE, SEONGHEE - University Of Florida
item RIOUX, RENEE - University Of Wisconsin
item Jia, Melissa
item Jia, Yulin
item MYSORE, KIRANKUMAR - Noble Research Institute

Submitted to: Australasian Plant Pathlogy Society
Publication Type: Proceedings
Publication Acceptance Date: 11/2/2021
Publication Date: 11/22/2021
Citation: Singh, P., Oh, Y., Lee, S., Rioux, R., Jia, M.H., Jia, Y., Mysore, K.S. 2021. Deciphering the role of ATP2-Binding Cassette (ABC) transporter candidate genes in rice sheath blight resistance. Abstract. Australasian Plant Pathlogy Society Virtual Workshop, November 3-6, 2021.

Interpretive Summary:

Technical Abstract: Sheath blight is one of the most damaging diseases of rice worldwide caused by Rhizoctonia solani, while host resistance mechanisms remain unclear. In this study, a virulent field isolate of Rhizoctonia solani was used to inoculate detached leaves of the resistant rice cultivar ‘Jasmine 85’, a suppression subtractive cDNA library was constructed using RNA isolated 16 hours post inoculation (hpi), and differentially expressed genes were identified from the cDNA library. Coupled with gene expression studies using DNA microarray, 27 highly induced genes involved in signal transduction and defense responses were identified within 16 hpi suggesting that rapid expression of defense genes is likely one mechanism of resisting the pathogen. Among the 27 induced genes, one is an ATP2-Binding Cassette (ABC) transporter. Previously, three members of the ABC transporter gene family (OsABC1, OsABC9 and OsABC12), encoding pleiotropic drug resistance (PDR)-like ATP binding cassette (ABC) transporters, were mapped to different sheath-blight resistance QTL. Three high-resolution melting (HRM) markers for OsABC1, OsABC9 and OsABC12 were then developed to verify differential gene expression and distinguish alleles between the sheath blight susceptible cultivar ‘Lemont’ and Jasmine 85. Association of sheath blight resistance with the presence of OsABC1, OsABC9 and OsABC12 was examined in 77recombinant inbred lines derived from the cross between Jasmine 85 and Lemont. The OsABC9 gene, located in a major sheath blight resistance QTL qShB9-2, showed a major contribution to sheath-blight resistance. Outcomes of this study are useful for marker assisted selection in developing sheath blight resistant lines in breeding programs and functional validation of the ABC genes in sheath blight disease resistance.