Skip to main content
ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Production and Genetic Improvement Research Unit » Research » Publications at this Location » Publication #390527
Research Project: Improved Fruit, Grape and Wine Products through Precision Agriculture and Quality Component Evaluation

Location: Horticultural Crops Production and Genetic Improvement Research Unit

Title: Virome association with common grape diseases in the development of new detection tools: year 1

Author
item KARASEV, A - University Of Idaho
item DAHAN, J - University Of Idaho
item Lee, Jungmin

Submitted to: Northwest Center for Small Fruit Research Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 12/20/2021
Publication Date: 12/22/2021
Citation: Karasev, A., Dahan, J., Lee, J. 2021. Virome association with common grape diseases in the development of new detection tools: year 1. Northwest Center for Small Fruit Research Proceedings. Virtual, Corvallis, OR December 2021.

Interpretive Summary:

Technical Abstract: The proposed study is aimed at: (i) characterization of the virome associated with the grapevine disease complexes in fields; (ii) development of specific tools for detection and differentiation of newly described species and strains of important viruses; (iii) use of the developed tools to survey PNW states, to get a comprehensive picture of the presence of the novel virus strains;(iv) and development of broad-spectrum diagnostic tools like RT-PCR to allow detection in the field. In the first year of the project, we collected 50 leaf and petiole samples from symptomatic grapevine plants in six vineyards in Canyon and Nez Perce counties of Idaho, and subjected them to RT-PCR testing for multiple grapevine viruses. We identified samples carrying up to 18 viruses and virus strains, and prepared ribosomal RNA-depleted RNA extracts for the high-throughput sequencing (HTS). The HTS cDNA libraries are currently being sequenced.