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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Produce Safety and Microbiology Research » Research » Publications at this Location » Publication #390828
Research Project: Elucidating the Factors that Determine the Ecology of Human Pathogens in Foods

Location: Produce Safety and Microbiology Research

Title: Top-down identification of Shiga toxin (and other virulence factors and biomarkers) from pathogenic E. coli using MALDI-TOF-TOF tandem mass spectrometry

Author
item Fagerquist, Clifton - Keith

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 1/25/2022
Publication Date: 3/20/2023
Citation: Fagerquist, C.K. 2023. Top-down identification of Shiga toxin (and other virulence factors and biomarkers) from pathogenic E. coli using MALDI-TOF/TOF tandem mass spectrometry. In: Shah, H.N., Gharbia, S.E., Shah, A.J., Tranfield, E.Y., Thompson, K.C., editors. Microbiological Identification Using MALDI-TOF and Tandem Mass Spectrometry: Industrial and environmental applications. 1st edition. West Sussex, UK: John Wiley & Sons Ltd. p. 71-96. https://doi.org/10.1002/9781119814085.ch3.
DOI: https://doi.org/10.1002/9781119814085.ch3

Interpretive Summary:

Technical Abstract: This chapter details the advantages and limitations of matrix-assisted laser desorption/ionization time-of-flight-time-of-flight (MALDI-TOF-TOF) tandem mass spectrometry (MS/MS) for top-down proteomic identification of bacterial proteins including Shiga toxin from Shiga toxin-producing Escherichia coli (STEC). Topics discussed: the utility of post-source decay (PSD) as a dissociation technique; the aspartic acid effect (AAE) and residue-specific top-down proteomic analysis; protein denaturation and its effect on protein collision cross-section, energy deposition and protein ion fragmentation; antibiotic induction and gene expression of target proteins for top-down proteomic analysis.