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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Production and Genetic Improvement Research Unit » Research » Publications at this Location » Publication #392995
Research Project: Improved Fruit, Grape and Wine Products through Precision Agriculture and Quality Component Evaluation

Location: Horticultural Crops Production and Genetic Improvement Research Unit

Title: Occurrence of grapevine rupestris vein feathering virus in Idaho wine grapes

Author
item DAHAN, JENNIFER - University Of Idaho
item THOMPSON, BRANDON - University Of Idaho
item Lee, Jungmin
item KARASEV, ALEXANDER - University Of Idaho

Submitted to: APS Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 6/14/2022
Publication Date: 8/6/2022
Citation: Dahan, J., Thompson, B., Lee, J., Karasev, A. 2022. Occurrence of grapevine rupestris vein feathering virus in Idaho wine grapes. APS Annual Meeting. Plant health 2022. Pittsburgh, PA. Abstract # 20718.

Interpretive Summary:

Technical Abstract: Grapevine rupestris vein feathering virus (GRVFV) was found associated with chlorotic discolorations of leaf veins in a Greek grapevine cultivar or with Syrah decline. In the United States, GRVFV was reported to occur in California and Washington State. Wine grape production in Idaho is known to be affected by several viruses but the GRVFV status was not addressed previously. In 2018, leaf and petiole samples from five declining Chardonnay vines were collected from a single vineyard in Canyon County of Idaho. Ribodepleted total RNA prepared from these samples was subjected to a high-throughput sequencing (HTS) analysis on a MiSeq platform and all five samples were found to contain GLRaV-3 and the two common viroids, while contigs exhibiting homology with the GRVFV were found in three out of the five Chardonnay samples analyzed. A nearly complete genome of GRVFV-ID was assembled from the HTS data of one sample, and the 3’-terminus of the genome was acquired using the RACE methodology. In the fall of 2020, six commercially operating vineyards in Canyon and Nez Perce Counties of Idaho, including the original one, were sampled based on visual symptoms of leaf reddening, leaf rolling, and chlorosis, and tested by RT-PCR. GRVFV-positive plants were found in three vineyards in Canyon County, representing the same wine grape cultivar, Chardonnay. The close identity, over 98%, between the GRVFV sequences from three different Idaho vineyards, coming from the same cultivar Chardonnay, may suggest a common origin of the original GRVFV infection, possibly the same supplier of the original Chardonnay planting material or shared cuttings. Presence of GRVFV might have contributed to the decline of the original Chardonnay vines, although the exact role of GRVFV in a mixed infection with GLRaV-3 is not clear at the moment.