Effects of environmental temperatures on Culicoides vector physiology and vesicular stomatitis virus infection

Authors: Drolet, Barbara; ROZO-LOPEZ, PAULA - University Of Tennessee

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/15/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary: Interpretive Summary not required in accordance with ARS-115 Publications P & P 152.1 v.5 (10/19/2019) chapter 5 page 31 Matrix for Data Entry Determinations. Kmm

Technical Abstract: Culicoides biting midges are nuisance pests of livestock and well-known vectors of veterinary arboviruses, such as vesicular stomatitis virus (VSV). Female midges ingest viruses when feeding on blood to obtain protein for egg-laying. After ingesting a VSV-infected blood meal, the environmental temperature of the resting location mediates the rates at which blood is digested, eggs are laid, and virus particles are replicated inside the midge. VSV transmission will occur if the timing of virus amplification aligns with the next feeding–egg-laying cycle. We evaluated the impact of constant environmental temperatures on midge physiology (lifespan and reproduction), vector competence for VSV (infection and dissemination), and thermal resting preference. Our results indicate that after ingesting a blood meal, most midges prefer to rest in areas that fall within their preferred physiological range regardless of the temperatures at which they were being maintained.These preferred temperatures maximized their survival, the number of egg-laying cycles, and the likelihood of VSV transmission. Our temperature approach shows that in the Culicoides–VSV system, the preferred resting temperature selected by blood-fed midges is beneficial for both insect and virus transmission.