In vitro antibacterial and antifungal efficacy assessment of ethanolic and aqueous extracts of Rumex nervosus leaves against selected bacteria and fungi

Authors: AL-GARADI, AHMED - Tharmar University; QUID-MOHAMMED, MABKHUT - Tharmar University; ALQHTANI-ABDULMOHSEN, HUSSEN - King Saud University; Pokoo-Aikins, Anthony; AL-MUFARREJ-SAUD, IBRAHIM - King Saud University

Submitted to: Veterinary world
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/26/2022
Publication Date: 11/29/2022
Citation: Al-Garadi, A., Quid-Mohammed, M., Alqhtani-Abdulmohsen, H., Pokoo-Aikins, A., Al-Mufarrej-Saud, I. 2022. In vitro antibacterial and antifungal efficacy assessment of ethanolic and aqueous extracts of Rumex nervosus leaves against selected bacteria and fungi. Veterinary world. 15(11):2725-2737. https://doi.org/10.14202/vetworld.2022.2725-2737.
DOI: https://doi.org/10.14202/vetworld.2022.2725-2737

Interpretive Summary: Traces of antibiotics in animal products are a major health problem worldwide. There is a global search for natural medicines that kill microbes. The medicines must work against drug-resistant microbes that cause disease and fungi that cause things to spoil. Rumex nervosus leaves (RNL) were collected and extracted for the natural compounds in the leaves. Each extract was used on four bacterial and two fungal strains. The pure ethanol extract was the best against bacteria. The 50% ethanol extract showed the greatest inhibition of growth against certain bacteria. The water extract was the strongest against fungal growth. RNL extracts stopped microbial growth to varying extents. The results of this study suggest that RNL extract is a promising new natural antimicrobial agent for preserving food.

Technical Abstract: Scientists are interested in looking for natural antibiotic substitutes that are effective against drug-resistant pathogenic microbes and spoilage fungi to counteract pathogens and reduce the major public health problem of antibiotic residues in animal products. Rumex nervosus leaves (RNL) as medicinal herb were tested for their antimicrobial activity against four bacterial and two fungal strains using absolute ethanol, 50% ethanol and aqueous extracts. Disc-diffusion antibiotic susceptibility test, minimal inhibitory and minimal bactericidal or fungicidal concentrations were used to evaluate antimicrobial activity as well-poisoned fungicidal food method. The absolute ethanol-RNL extract showed the best bacteriostatic/bactericidal, the 50% ethanol-RNL extract showed the greatest zone of inhibition against bacterial isolates, and the water extract of RNL were the strongest inhibition of fungal mycelial growth. RNL extracts inhibited microbial growth to varying degrees. The results of the present study suggest that RNL is promising novel natural antimicrobial agents for food preservation. To date, most research on the antimicrobial properties of natural herbs have been conducted in vitro, with a few exceptions involving in vivo and intervention-based research.