Location: Crop Improvement and Genetics Research
Title: Updated annotation of Affymetrix wheat genome array microarray probesAuthor
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WAYMACK, RACHEL - Oak Ridge Institute For Science And Education (ORISE) |
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L Chingcuanco, Debbie |
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Submitted to: Survey Notes
Publication Type: Research Notes Publication Acceptance Date: 9/12/2022 Publication Date: 9/12/2022 Citation: Waymack, R.V., Chingcuanco, D.L. 2022. Updated annotation of Affymetrix wheat genome array microarray probes. Annual Wheat Newsletter. 68:32-33. Available: https://wheat.pw.usda.gov/ggpages/awn/68/AWN_VOL_68.pdf Interpretive Summary: . Technical Abstract: The Affymetrix Wheat Genome Array is a widely used set of microarray probes designed to target over 55,000 transcripts across the wheat genome. This set of probes continues to be used to understand wheat transcriptional activity across a wide range of conditions including drought (Kumar et al. 2018), seed development (Tuan et al, 2019), and viral infection (Kumar et al, 2021). Although a still very useful resource, the Wheat Genome Array probes were designed and annotated prior to the assembly and release of the completed reference wheat genome (Appels et al. 2018). With the completed reference wheat genome, and particularly the most recently updated version (Zhu et al. 2021), many original annotations have become outdated, whereas other probes initially lacking annotations may now be able to accurately be assigned to known genes (De Leeuw et al. 2008). An updated annotation of the Affymetrix Wheat Genome Array probes would improve the knowledge gained by studies utilizing these microarray probes by providing a more accurate picture of the genes and corresponding functions differentially regulated in any tested condition. |
