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ARS Home » Pacific West Area » Corvallis, Oregon » Horticultural Crops Production and Genetic Improvement Research Unit » Research » Publications at this Location » Publication #399143
Research Project: Improved Fruit, Grape and Wine Products through Precision Agriculture and Quality Component Evaluation

Location: Horticultural Crops Production and Genetic Improvement Research Unit

Title: Characterization of viral populations associated with common grape virus diseases in the Pacific Northwest and development of new detection tools

Author
item KARASEV, ALEXANDAR - University Of Idaho
item DAHAN, JENNIFER - University Of Idaho
item ORELLANA, GARDENIA - University Of Idaho
item Lee, Jungmin

Submitted to: Northwest Center for Small Fruit Research Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/10/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: In the first two years of this project, we collected over 100 leaf and petiole samples from symptomatic grapevine plants in eight vineyards in Canyon and Nez Perce counties of Idaho and subjected them to high-throughput sequencing (HTS) and RT-PCR testing with the goal to uncover the entire virome associated with grapevine diseases and abnormalities. Multiple grapevine viruses were uncovered by HTS, and their presence was confirmed and validated by RT-PCR with specific primers designed based on the information obtained with HTS. Up to 18 viruses and virus strains, in addition to several genetic variants of hop stunt viroid and grapevine yellow speckle viroid 1, were identified in some declining ‘Chardonnay’ vines from Canyon County, Idaho. Two viruses were reported from Idaho for the first time, grapevine rupestris vein feathering virus (GRVFV) and grapevine-associated tymo-like virus (GaTLV), both found associated with decline observed in ‘Chardonnay’. For GaTLV, this is also the first confirmed report for the United States.