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ARS Home » Pacific West Area » Logan, Utah » Forage and Range Research » Research » Publications at this Location » Publication #408457
Research Project: Improved Plant Genetic Resources and Methodologies for Rangelands, Pastures, and Turf Landscapes in the Semiarid Western U.S.

Location: Forage and Range Research

Title: Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH

Author
item QI, FEI - SHANDONG AGRICULTURAL UNIVERSITY
item LIANG, SHUANG - SHANDONG AGRICULTURAL UNIVERSITY
item BAO, YINGUANG - SHANDONG AGRICULTURAL UNIVERSITY
item Wang, Richard
item LI, XINGFENG - SHANDONG AGRICULTURAL UNIVERSITY
item XING, PIYI - SHANDONG AGRICULTURAL UNIVERSITY

Submitted to: Plants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/20/2023
Publication Date: 10/27/2023
Citation: Qi, F., Liang, S., Bao, Y., Wang, R., Li, X., Xing, P. 2023. Genome Analysis of Thinopyrum intermedium and Its Potential Progenitor Species Using Oligo-FISH. Plants. https://doi.org/10.3390/plants12213705.
DOI: https://doi.org/10.3390/plants12213705

Interpretive Summary: Intermediate wheatgrass is an important worldwide forage crop and a valuable gene reservoir for wheat improvement. Its genome composition is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of intermediate wheatgrass and its relative diploid species. St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum were used as probes in fluorescence in situ hybridization (FISH) to classified the chromosomes of Intermediate wheatgrass into three groups, expressed as JvsJvsJrJrStSt. A combined multiplex oligonucleotide probes was used to establish the FISH karyotype of ten accessions of Th. intermedium. Variability among and within the studied accessions of Intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that Jvs had largely been contributed from Da. breviaristatum, but not the present-day Da. villosum; Jr was related to either Th. elongatum or Th. bessarabicum; and St was contributed from the genus Pseudoroegneria by hybridization with Th. junceiforme or Th. sartorii.

Technical Abstract: Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey) is an important worldwide forage crop and a valuable gene reservoir for wheat improvement. Its genome composition is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of Th. intermedium and its relative diploid species. St2-80 developed from Pseudowroegneria strigose and pDb12H developed from Dasypyrum breviaristatum were used as probes in fluorescence in situ hybridization (FISH) to classified the chromosomes of Th.intermedium into three groups, expressed as JvsJvsJrJrStSt. A combined multiplex oligonucleotide probes, including pSc119.2-1, (GAA)10, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4 and pAs1-6, was used to establish the FISH karyotype of ten accessions of Th. intermedium. Variability among and within the studied accessions of intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that Jvs had largely been contributed from Da. breviaristatum, but not the present-day Da. villosum; Jr was related to either Th. elongatum or Th. bessarabicum; and St was contributed from the genus Pseudoroegneria by hybridization with Th. junceiforme or Th. sartorii.