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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Improvement Research » Research » Publications at this Location » Publication #409287
Research Project: Improvement of Disease and Pest Resistance in Barley, Durum, Oat, and Wheat Using Genetics and Genomics

Location: Cereal Crops Improvement Research

Title: Medicago truncatula resources to study legume biology and symbiotic nitrogen fixation

Author
item Nandety, Raja Sekhar
item WEN, JIANGQI - Oklahoma State University
item MYSORE, KIRANKUMAR - Oklahoma State University

Submitted to: Fundamental Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/19/2022
Publication Date: 3/3/2023
Citation: Nandety, R., Wen, J., Mysore, K. 2023. Medicago truncatula resources to study legume biology and symbiotic nitrogen fixation. Fundamental Research. 3(2):219-224. https://doi.org/10.1016/j.fmre.2022.06.018.
DOI: https://doi.org/10.1016/j.fmre.2022.06.018

Interpretive Summary: Legumes play a key role in the sustainable agriculture to fix atmospheric nitrogen through a mutualistic symbiotic process with nitrogen-fixing bacteria. Tnt1, a retrotransposon from tobacco, was used to generate insertion mutants in M. truncatula R108. Approximately,'~21,000 insertion lines have been generated and publicly available. A variety of genomic resources available to the community including expression atlas, small secreted peptides, genome sequencing information, mutant resources and metabolic pathways were updated and reviewed.

Technical Abstract: Medicago truncatula is a chosen model for legumes towards deciphering fundamental legume biology, especially symbiotic nitrogen fixation. Current genomic resources for M. truncatula include a completed whole genome sequence information for R108 and Jemalong A17 accessions along with the sparse draft genome sequences for other 226 M. truncatula accessions. These genomic resources are complemented by the availability of mutant resources such as retrotransposon (Tnt1) insertion mutants in R108 and fast neutron bombardment (FNB) mutants in A17. In addition, several M. truncatula databases such as small secreted peptides (SSPs) database, transporter protein database, gene expression atlas, proteomic atlas, and metabolite atlas are available to the research community. This review describes these resources and provide information regarding how to access these resources.