Skip to main content
ARS Home » Southeast Area » Stuttgart, Arkansas » Dale Bumpers National Rice Research Center » Research » Publications at this Location » Publication #418067
Research Project: Broadening and Strengthening the Genetic Base of Rice for Adaptation to a Changing Climate, Crop Production Systems, and Markets

Location: Dale Bumpers National Rice Research Center

Title: The effects of abiotic stressors on rice resistance responses to the blast fungus Magnaporthe oryzae

Author
item HUANG, YIXIAO - Oak Ridge Institute For Science And Education (ORISE)
item Jia, Yulin

Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 8/17/2024
Publication Date: 8/17/2024
Citation: Huang, Y., Jia, Y. 2024. The effects of abiotic stressors on rice resistance responses to the blast fungus Magnaporthe oryzae. Abstract. 2024 International Symposium on Rice Functional Genomics, September 9-11, 2024. Little Rock/Stuttgart, Arkansas.

Interpretive Summary:

Technical Abstract: Rice, a key staple food crop worldwide, faces multiple abiotic and biotic stresses. The common abiotic stresses for rice include heat, cold, drought and salinity. The common biotic stresses are the fungal diseases including rice blast disease. Rice blast, caused by the fungus Magnaporthe oryzae, is one of the most damaging diseases for rice globally. The relationship between abiotic stresses and blast disease resistance responses remains unclear. To investigate whether these abiotic stresses influence plants resistance against blast pathogen and which blast resistance (R) genes in plants are effective under extreme conditions, rice cultivars with different combinations of R genes were exposed to multiple abiotic stresses followed by blast inoculation. Pathogenesis related gene 1 (PR1) and PR10 in rice were used as markers for defense responses of rice to stresses. Preliminary experimental trials revealed that rice could recover after 10 days of drought, 3 days of salinity (200 mM NaCl), 4 hours of heat (45°C), and 8 hours of cold (4°C). In one experiment, rice plants were exposed to 4°C for 8 hours before being inoculated with the blast pathogen and the expression of pathogenesis-related genes, Expressions of PR1 and PR10 were analyzed using real time PCR at different time points after pathogen inoculation. In another experiment, rice plants underwent an 8-hour cold treatment at 10°C before blast inoculation, with the severity of disease development subsequently assessed. Progress of these experiments will be presented.