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Title: CONSTRUCTION OF A PORCINE YEAST ARTIFICIAL CHROMOSOME (YAC) LIBRARY

Author
item Alexander, Leeson
item Smith, Timothy - Tim
item Rohrer, Gary
item Beattie, Craig
item BROOM, MURREY - UNIV OTAGO, NEW ZEALAND

Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/1996
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The recent construction of three genetic linkage maps of the porcine genome allows the assignment of loci affecting heritable traits of economic importance to specific chromosomal segments. Markers can thus be identified that may be useful in marker assisted selection (MAS) to increase the frequency of favorable allele(s) in resource populations. In addition, mapping of these loci creates the opportunity to identify gene(s) influencing a trait, through positional cloning or positional candidate gene approaches. The positional cloning approach requires the construction of contigs that physically span large sections of chromosomes. To facilitate the construction of these contigs, we have generated a Yeast Artificial Chromosome (YAC) library, in the vector pYAC4, containing large porcine genomic fragments. The library consists of 33,120 clones containing a median size of 480 kb. This represents a 5.5 fold coverage of the porcine genome assuming a genome size of 3x10^9 bp. Initial assessment of chimerism by fluorescence in situ hybridization (FISH) of clones yielded a chimerism frequency of 30%. We are presently isolating YACs that by genetic linkage analysis fall into regions of the genome that have few physical assignments. To date, we have assigned SW1416 to SSC15q1.1-1.2, SW1065 to SSC15q2.2 and SW1983 to SSC15q2.6.