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Title: ANALYTICAL SFE OF FAT: GC-FAME DETERMINATIONS FOR VALIDATION OF GRAVIMETRICMETHODS

Author
item Eller, Fred
item King, Jerry
item MYER, LESLIE - LECO CORPORATION

Submitted to: Association Official Analytical Chemists Annual Intrl Meeting & Exposition
Publication Type: Abstract Only
Publication Acceptance Date: 9/13/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Gravimetric and gas chromatographic fatty acid methyl ester (GC FAME) fat determination of supercritical CO2 (SC CO2) extracts for several food matrices were compared. Five oilseeds, three ground beef samples, five bakery samples, and a NIST Standard Reference Material (SRM) 1544 were extracted with SC CO2 and ethanol. The collected material was weighed and total fat determined gravimetrically (SFE GRAV). Subsequently, an internal standard was added and the material converted to FAME and analyzed by GC to determine total fat (SFE GC FAME). For sunflower and cottonseed, the SFE GRAV results were higher than the SFE GC FAME results, whereas the two methods were equivalent for soybeans, canola, and safflower. For the ground beef samples, the SFE GRAV results were significantly higher than the GC FAME results. For the high fat bakery samples, the SFE GRAV and SFE GC FAME methods were equivalent. The SFE GRAV method overestimated the fat content of the low fat bread and cake. For SRM 1544, the SFE GRAV result was significantly higher than the SFE GC FAME result.