Research Project: Advanced Development of Innovative Technologies and Systematic Approaches to Foodborne Hazard Detection and Characterization for Improving Food Safety

Location: Characterization and Interventions for Foodborne Pathogens

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Detection of E. coli labeled with metal-conjugated antibodies using lateral-flow assay and laser-induced breakdown spectroscopy - (Peer Reviewed Journal)
Gondhalekar, C., Biela, E., Rajwa, B.B., Patsekin, V., Sturgis, J., Reynolds, C., Doh, I.J., Diwakar, P., Stanker, L., Zorba, V., Mao, X., Russo, R., Robinson, J.P. 2020. Detection of E. coli labeled with metal-conjugated antibodies using lateral-flow assay and laser-induced breakdown spectroscopy. Analytical and Bioanalytical Chemistry. 412:1291-1301. https://doi.org/10.1007/s00216-019-02347-3.

Microbes as a tool to defend against antibiotic resistance in food animal production - (Peer Reviewed Journal)
Bhunia, A.K. 2019. Microbes as a tool to defend against antibiotic resistance in food animal production. Indian Journal of Animal Sciences. 58(2). https://doi.org/10.36062/ijah.58.2SPL.2019.01-18.

Gold nanostars for the detection of foodborne pathogens via surface-enhanced raman scattering combined with microfluidics - (Peer Reviewed Journal)
Rodriquez-Lorenzo, L., Garrido-Maestu, A., Bhunia, J.K., Espina, B., Prado, M., Diéguez, L., Abaldecela, S. 2019. Gold nanostars for the detection of foodborne pathogens via surface-enhanced raman scattering combined with microfluidics. ACS Nano. 2(10):6081-6086. https://doi.org/10.1021/acsanm.9b01223.

Prevalence and antimicrobial resistance of Campylobacter from antibiotic-free broilers during organic and conventional processing - (Peer Reviewed Journal)
Bailey, M.A., Taylor, R.M., Brar, J.S., Corkran, S.C., Velasquez, C., Novoa, R.E., Oliver, H.F., Singh, M. 2019. Prevalence and antimicrobial resistance of Campylobacter from antibiotic-free broilers during organic and conventional processing. Poultry Science. 98(3):1447-1454. https://doi.org/10.3382/ps/pey486.

Animal-use antibiotics induce cross-resistance in bacterial pathogens to human therapeutic antibiotics - (Peer Reviewed Journal)
Singh, A.K., Bhunia, A. 2019. Animal-use antibiotics induce cross-resistance in bacterial pathogens to human therapeutic antibiotics. Current Microbiology. 76:1112–1117. https://doi.org/10.1007/s00284-019-01744-2.

Aptamer-based SERS biosensor for whole cell analytical detection of E. coli O157:H7 - (Peer Reviewed Journal)
Diaz-Amaya, S., Lin, L.K., Stanciu, L.A. 2019. Aptamer-based SERS biosensor for whole cell analytical detection of E. coli O157:H7. Analytica Chimica Acta. 1080:131-137. https://doi.org/10.1016/j.aca.2019.06.046.

Inkjet printed electrochemical aptasensor for detection of Hg2+ in organic solvents - (Peer Reviewed Journal)
Diaz-Amaya, S., Lin, L., Dinino, R.E., Ostos, C., Stanciu, L.A. 2019. Inkjet printed electrochemical aptasensor for detection of Hg2+ in organic solvents. Electrochemical Acta. 316:33-42. https://doi.org/10.1016/j.electacta.2019.05.079.

Three dimensional vero cell-platform for rapid and sensitive screening of Shiga-Toxin producing Escherichia coli - (Peer Reviewed Journal)
To, C.Z., Bhunia, A.K. 2019. Three dimensional vero cell-platform for rapid and sensitive screening of Shiga-Toxin producing Escherichia coli. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2019.00949.

Lactobacillus casei expressing internalins A and B reduces Listeria monocytogenes interaction with Caco-2 cells in vitro - (Peer Reviewed Journal)
Mathipa, M.G., Thantsha, M.S., Bhunia, A.K. 2019. Lactobacillus casei expressing internalins A and B reduces Listeria monocytogenes interaction with Caco-2 cells in vitro. Microbial Biotechnology. 12(4):715-729.

A portable spark-induced breakdown spectroscopic (SIBS) instrument and its analytical performance - (Peer Reviewed Journal)
Doh, J., Gondhalekar, C., Patsekin, V., Rajwa, B., Hernandez, K., Bae, E., Robinson, J.P. 2019. A portable spark-induced breakdown spectroscopic (SIBS) instrument and its analytical performance. Applied Spectroscopy. 73(6):698-708. https://doi.org/10.1177/0003702819844792.

A portable spark-induced breakdown spectroscopic (SIBS) instrument and its analytical performance - (Peer Reviewed Journal)
Doh, I., Gondhalekar, C., Patsekin, V., Rajwa, B., Hernandez, K., Bae, E., Robinson, J.P. 2019. A portable spark-induced breakdown spectroscopic (SIBS) instrument and its analytical performance. Applied Spectroscopy. 73(6):698-708. https://doi.org/10.1177/0003702819844792.

Inkjet printed nanopatterned aptamer-based sensors for improved optical detection of foodborne pathogens - (Peer Reviewed Journal)
Diaz Amaya, S., Zhao, M., Lin, L., Ostos, C., Allebach, J.P., Chiu, G.T., Deering, A., Stanciu, L.A. 2019. Inkjet printed nanopatterned aptamer-based sensors for improved optical detection of foodborne pathogens. Nano Micro Small. 15(24):e1805342. https://doi.org/10.1002/smll.201805342.

Plasmonic enhancement in lateral flow sensors for improved sensing of E. coli O157:H7 - (Peer Reviewed Journal)
Ren, W., Ballou, D.R., Fitzgerald, R., Irudayaraj, J. 2018. Plasmonic enhancement in lateral flow sensors for improved sensing of E. coli O157:H7. Biosensors and Bioelectronics. 126:324-331. https://doi.org/10.1016/j.bios.2018.10.066.

Antilisterial and antibiofilm activities of pediocin and LAP functionalized gold nanoparticles - (Peer Reviewed Journal)
Singh, A.K., Bai, X., Amalaradjou, M.A., Bhunia, A.K. 2018. Antilisterial and antibiofilm activities of pediocin and LAP functionalized gold nanoparticles . Frontiers in Sustainable Food Systems. https://doi.org/10.3389/fsufs.2018.00074.

Prevalence and antimicrobial resistance of Campylobacter from antibiotic-free broilers during organic and conventional processing - (Peer Reviewed Journal)
Bailey, M.A., Taylor, R.M., Brar, J.S., Corkran, S., Velasquez, C., Rama, E., Oliver, H., Singh, M. 2018. Prevalence and antimicrobial resistance of Campylobacter from antibiotic-free broilers during organic and conventional processing. Poultry Science. 98:1447-1454. https://doi.org/10.3382/ps/pey486.

Rapid detection and differentiation of Staphylococcus colonies using an optical scattering technology - (Peer Reviewed Journal)
Alsulami, T.S., Zhu, X., Abdelhaseib, M.U., Singh, A.K., Bhunia, A.K. 2018. Rapid detection and differentiation of Staphylococcus colonies using an optical scattering technology. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-018-1133-4.

Comparison of processing parameters in small and very small beef processing plants and their impact on Escherichia coli prevalence - (Peer Reviewed Journal)
Corkran, S.C., Bailey, M., Brar, J., Velasquez, C., Waddell, J., Oliver, H., Bratcher, C.L., Wang, L., Kumar, S., Singh, M. 2018. Comparison of processing parameters in small and very small beef processing plants and their impact on Escherichia coli prevalence. LWT - Food Science and Technology. 95:92-98. https://doi.org/10.1016/j.lwt.2018.04.041.

Prevalence and antimicrobial resistance patterns of Salmonella isolated from poultry farms in southeastern United States - (Peer Reviewed Journal)
Velasquez, C.G., Macklin, K.S., Kumar, S., Bailey, M., Ebner, P.E., Oliver, H.F., Martin-Gonzalez, F.S., Singh, M. 2018. Prevalence and antimicrobial resistance patterns of Salmonella isolated from poultry farms in southeastern United States. Poultry Science. 97(6):2144-2152. https://doi.org/10.3382/ps/pex449.

A closer look at Sporidiobolales: Ubiquitous microbial community members of plant and food biospheres - (Peer Reviewed Journal)
Urbina, H., Aime, M. 2018. A closer look at Sporidiobolales: Ubiquitous microbial community members of plant and food biospheres. Mycologia. 110(1):79-92.

Exploring the anti-quorum sensing activity of a D-limonene nanoemulsion for Escherichia coli O157:H7 - (Peer Reviewed Journal)
Wang, R., Vega, P., Xu, Y., Chen, C., Irudayaraj, J. 2018. Exploring the anti-quorum sensing activity of a D-limonene nanoemulsion for Escherichia coli O157:H7. Biomedical Materials Research. https://doi.org/10.1002/jbm.a.36404.

Evaluation of thermal process lethality for non-pathogenic Escherichia coli as a surrogate for Salmonella in ground beef - (Peer Reviewed Journal)
Redemann, M.A., Brar, J., Niebuhr, S.E., Lucia, L.M., Acuff, G.R., Dickson, J.S., Singh, M. 2017. Evaluation of thermal process lethality for non-pathogenic Escherichia coli as a surrogate for Salmonella in ground beef. LWT - Food Science and Technology. 90:290-296.

Evaluation of thermal process lethality for non-pathogenic Escherichia coli as a surrogate for Salmonella in ground beef - (Peer Reviewed Journal)
Redemann, M.A., Brar, J., Niebuhr, S.E., Lucia, L.M., Acuff, G.R., Dickson, J.S., Singh, M. 2017. Evaluation of thermal process lethality for non-pathogenic Escherichia coli as a surrogate for Salmonella in ground beef. LWT - Food Science and Technology. 90:290-296. https://doi.org/10.1016/j.lwt.201712.037.

An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli - (Review Article)
Wang, R., Xu, Y., Liu, H., Peng, J., Irudayaraj, J., Cui, F. 2017. An integrated microsystem with dielectrophoresis enrichment and impedance detection for detection of Escherichia coli. Biomedical Microdevices. 19(2):34.

Characterization of a 1024 × 1024 DG-BioFET platform - (Peer Reviewed Journal)
Duarte-Guevara, C., Swaminathan, V., Reddy Jr, B., Wen, C., Huang, Y., Huang, J., Liu, Y., Bashir, R. 2017. Characterization of a 1024 × 1024 DG-BioFET platform. Sensors and Actuators B: Chemical. 250:100-110.

A net fishing enrichment strategy for colorimetric detection of E. coli O157:H7 - (Peer Reviewed Journal)
Ren, W., Liu, W., Irudayaraj, J. 2017. A net fishing enrichment strategy for colorimetric detection of E. coli O157:H7. Sensors and Actuators B: Chemical. 247:923-929.

Human pathogens in plant biofilms: Formation, physiology, and detection - (Review Article)
Ximenes, E., Hoagland, L., Ku, S., Ladisch, M. 2017. Human pathogens in plant biofilms: Formation, physiology, and detection. Biotechnology and Bioengineering. 114:1403–1418.

Effect-size measures as descriptors of assay quality in high-content screening: A brief review of some available methodologies - (Review Article)
Rajwa, B. 2017. Effect-size measures as descriptors of assay quality in high-content screening: A brief review of some available methodologies. Assay and Drug Development Technologies. 15(1):15-29.

Smartphone-based low light detection for bioluminescence application - (Peer Reviewed Journal)
Kim, H., Jung, Y., Doh, I., Lozano-Mahecha, R.A., Applegate, B., Bae, E. 2017. Smartphone-based low light detection for bioluminescence application. Scientific Reports. doi: 10.1038/srep40203.

Colorimetric analysis of saliva–alcohol test strips by smartphone-based instruments using machine-learning algorithms - (Review Article)
Kim, H., Awofeso, O., Choi, S., Jung, Y., Bae, E. 2016. Colorimetric analysis of saliva–alcohol test strips by smartphone-based instruments using machine-learning algorithms. Applied Optics. doi: org/10.1364/AO.56.000084.

Listeria monocytogenes internalizes in Romaine Lettuce grown in greenhouse conditions - (Peer Reviewed Journal)
Shenoy, A.G., Oliver, H.F., Deering, A.J. 2017. Listeria monocytogenes internalizes in Romaine Lettuce grown in greenhouse conditions. Journal of Food Protection. 80(4):573–581.

On-chip electrical detection of parallel loop-mediated isothermal amplification with DG-BioFETs for the detection of foodborne bacterial pathogens - (Popular Publication)
Duarte-Guevara, C., Swaminathan, V.V., Reddy, B., Huang, J.C., Liu, Y.S., Bashir, R. 2017. On-chip electrical detection of parallel loop-mediated isothermal amplification with DG-BioFETs for the detection of foodborne bacterial pathogens. eLife. doi: 10.1039/c6ra19685c.

Reflected scatterometry for noninvasive interrogation of bacterial colonies - (Peer Reviewed Journal)
Kim, H., Doh, I., Sturgis, J., Bhunia, A.K., Robinson, J.P., Bae, E. 2016. Reflected scatterometry for noninvasive interrogation of bacterial colonies. Journal of Biomedical Optics. doi: 10.1117/1.JBO.21.10.107004.

Microcantilevers track single-cell mass - (Review Article)
Bashir, R. 2016. Microcantilevers track single-cell mass. Nature Biotechnology. 34(11):1125-1126.

The use of a novel nanoLuc-based reporter phage for the detection of Escherichia coli O157:H7 - (Peer Reviewed Journal)
Zhang, D., Coronel-Aguilera, C., Romero, P., Perry, L., Minocha, U., Rosenfield, C., Gehring, A.G., Paoli, G., Bhunia, A.K., Applegate, B. 2016. The use of a novel nanoLuc-based reporter phage for the detection of Escherichia coli O157:H7. Scientific Reports. doi: 10.1038/srep33235.

Microfiltration of enzyme treated egg whites for accelerated detection of viable Salmonella - (Peer Reviewed Journal)
Ku, S., Ximenes, E., Kreke, T., Foster, K., Deering, A.J., Ladisch, M.R. 2016. Microfiltration of enzyme treated egg whites for accelerated detection of viable Salmonella. Biotechnology Progress. 32(6):1464-1471.

Fructose 1,6-Bisphosphate aldolase, a novel immunogenic surface protein on Listeria species - (Peer Reviewed Journal)
Mendonça, M., Moreira, G., Conceição, F., Hust, M., Mendonça, K., Moreira, Â., França, R., Padilha Da Silva, W., Bhunia, A.K., Aleixo, J. 2016. Fructose 1,6-Bisphosphate aldolase, a novel immunogenic surface protein on Listeria species. PLoS One. doi: 10.1371/journal.pone.0160544.

On-chip PMA labeling of foodborne pathogenic bacteria for viable qPCR and qLAMP detection - (Peer Reviewed Journal)
Duarte-Guevara, P., Duarte-Guevara, C., Ornob, A., Bashir, R. 2016. On-chip PMA labeling of foodborne pathogenic bacteria for viable qPCR and qLAMP detection. Microfluidics and Nanofluidics. doi: 10.1007/s104016-1778-2.

Development of a multispectral light-scatter sensor for bacterial colonies - (Peer Reviewed Journal)
Kim, H., Rajwa, B., Bhunia, A.K., Robinson, J.P., Bae, E. 2016. Development of a multispectral light-scatter sensor for bacterial colonies. Journal of Biophotonics. 10:634–644.

Droplet-based biosensing for lab-on-a-chip, open microfluidics platforms - (Review Article)
Dak, P., Ebrahimi, A., Swaminathan, V., Duarte-Guevara, C., Bashir, R., Alam, M.A. 2016. Droplet-based biosensing for lab-on-a-chip, open microfluidics platforms. Biosensors. doi: 10.3390/bios6020014.

Electrical chips for biological point-of-care detection - (Review Article)
Reddy, B., Salm, E., Bashir, R. 2016. Electrical chips for biological point-of-care detection. Annals of Biomedical Engineering. 18:329-355.