Research Project: Non-Antibiotic Strategies to Control Priority Bacterial Infections in Swine

Location: Virus and Prion Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Genome sequences of Streptococcus suis isolates obtained from pigs in the United States between 2015 and 2017 - (Rapid Release Publication)
Nicholson, T.L., Shore, S. 2022. Genome sequences of Streptococcus suis isolates obtained from pigs in the United States between 2015 and 2017. Microbiology Resource Announcements. 11(8):e01067-21. https://doi.org/10.1128/mra.01067-21.

Replication of Streptococcus equi subspecies zooepidemicus disease in swine - (Peer Reviewed Journal)
Hau, S.J., Brockmeier, S., Lantz, K., Stuart, K.L., Sitthicharoenchai, P., Macedo, N., Derscheid, R.J., Burrough, E.R., Robbe-Austerman, S. 2022. Replication of Streptococcus equi subspecies zooepidemicus disease in swine. Veterinary Microbiology. 264. https://doi.org/10.1016/j.vetmic.2021.109271.

Complete genome sequence of Escherichia coli Antibiotic-Resistance Isolate AR Bank #0346 - (Peer Reviewed Journal)
Stuart, K.L., Bayles, D.O., Shore, S., Nicholson, T.L. 2021. Complete genome sequence of Escherichia coli Antibiotic-Resistance Isolate AR Bank #0346. Microbiology Resource Announcements. 10(23). https://doi.org/10.1128/MRA.00305-21.

Importance of strain selection in the generation of heterologous immunity to Glaesserella (Haemophilus) parasuis - (Peer Reviewed Journal)
Hau, S.J., Eberle, K.C., Brockmeier, S. 2021. Importance of strain selection in the generation of heterologous immunity to Glaesserella (Haemophilus) parasuis. Veterinary Immunology and Immunopathology. 234. Article 110205. https://doi.org/10.1016/j.vetimm.2021.110205.

Comparative virulence and genomic analysis of streptococcus suis isolates - (Peer Reviewed Journal)
Nicholson, T.L., Waack, U., Anderson, T.K., Bayles, D.O., Zaia, S.R., Goertz, I., Eppinger, M., Hau, S.J., Brockmeier, S., Shore, S. 2021. Comparative virulence and genomic analysis of streptococcus suis isolates. Frontiers in Microbiology. 11. Article 620843. https://doi.org/10.3389/fmicb.2020.620843.

Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs - (Peer Reviewed Journal)
Hau, S.J., Luan, S., Loving, C.L., Nicholson, T.L., Wang, J., Peters, S.E., Seilly, D., Weinert, L., Langford, P.R., Rycroft, A., Wren, B.W., Maskell, D.J., Tucker, A.W., Brockmeier, S. 2020. Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs. BMC Veterinary Research. 16:167. https://doi.org/10.1186/s12917-020-02377-5.

Update on Streptococcus suis research and prevention in the era of antimicrobial restriction: 4th International Workshop on S. suis - (Peer Reviewed Journal)
Segura, M., Aragon, V., Brockmeier, S., Gebhart, C., De Greeff, ., Kerdsin, ., O’Dea, M.A., Okura, M., Saléry, M., Schults, C., Valentin-Weigand, P., Weinert, L.A., Wells, J.M., Gottschalk, M. 2020. Update on Streptococcus suis research and prevention in the era of antimicrobial restriction: 4th International Workshop on S. suis. Pathogens. 9(5). https://doi.org/10.3390/pathogens9050374.

Generation and evaluation of a Glaesserella parasuis capsular mutant - (Peer Reviewed Journal)
Eberle, K.C., Hau, S.J., Luan, S., Weinert, L., Stasko, J.A., Wang, J., Peters, S.E., Langford, P.R., Rycroft, A., Wren, B.W., Maskell, D.J., Tucker, A.W., Brockmeier, S. 2020. Generation and evaluation of a Glaesserella parasuis capsular mutant. Infection and Immunity. 88(5):e00879-19. https://doi.org/10.1128/IAI.00879-19.

Complete genome sequence of Bordetella bronchiseptica strain KM22 - (Peer Reviewed Journal)
Nicholson, T.L., Bayles, D.O., Shore, S. 2020. Complete genome sequence of Bordetella bronchiseptica strain KM22. Microbiology Resource Announcements. 9(4). https://doi.org/10.1128/MRA.01207-19.

Complete genome sequence of Escherichia coli antibiotic resistance (AR) isolate bank #0349 - (Peer Reviewed Journal)
Stuart, K.L., Shore, S., Nicholson, T.L. 2019. Complete genome sequence of Escherichia coli antibiotic resistance (AR) isolate bank #0349. Microbiology Resource Announcements. 8(48). https://doi.org/10.1128/MRA.01078-19.

Bordetella bronchiseptica and respiratory disease in swine - (Review Article)
Hau, S.J., Brockmeier, S. 2019. Bordetella bronchiseptica and respiratory disease in swine. Review Article. p.1-21.

Shifts in the swine nasal microbiota of swine in response to different dosing regimens of oxytetracycline administration - (Peer Reviewed Journal)
Mou, K.T., Allen, H.K., Alt, D.P., Trachsel, J.M., Hau, S.J., Coetzee, J.F., Holman, D.B., Kellner, S.G., Loving, C.L., Brockmeier, S. 2019. Shifts in the swine nasal microbiota of swine in response to different dosing regimens of oxytetracycline administration. Veterinary Microbiology. 237(1084020). https://doi.org/10.1016/j.vetmic.2019.108386.

Transcriptomic differences noted in Glasserella parasuis between growth in broth and on agar - (Peer Reviewed Journal)
Brockmeier, S., Hau, S.J., Mou, K.T., Bayles, D.O. 2019. Transcriptomic differences noted in Glasserella parasuis between growth in broth and on agar. PLoS One. 14(8):e0220365. https://doi.org/10.1371/journal.pone.0220365.

Potential use of G-CSF for protection against Streptococcus suis infection in swine - (Peer Reviewed Journal)
Brockmeier, S., Loving, C.L., Eberle, K.C., Hau, S.J., Mou, K.T., Kehrli Jr., M.E. 2019. Administration of granulocyte-colony stimulating factor (G-CSF) to pigs results in a longer mean survival time after exposure to Streptococcus suis. Veterinary Microbiology. 231:116-119. https://doi.org/10.1016/j.vetmic.2019.03.010.

Contribution of the BvgR and RisAS regulons to Bordetella bronchiseptica biology - (Abstract Only)

The Streptococcos suis sortases SrtB and SrtF are essential for disease in pigs - (Peer Reviewed Journal)
Faulds-Pain, A., Shaw, H.A., Terra, V.S., Kellner, S., Brockmeier, S L., Wren, B.W. 2018. The Streptococcos suis sortases SrtB and SrtF are essential for disease in pigs. Microbiology. 165:163-173. https://doi.org/10.1099/mic.0.000752.

Subinhibitory concentrations of amoxicillin, lincomycin, and oxytetracycline commonly used to treat swine increase Streptococcus suis biofilm formation - (Peer Reviewed Journal)
Waack, U., Nicholson, T.L. 2018. Subinhibitory concentrations of amoxicillin, lincomycin, and oxytetracycline commonly used to treat swine increase Streptococcus suis biofilm formation. Frontiers in Microbiology. 9:2707. https://doi.org/10.3389/fmicb.2018.02707.

Comparative genomic and methylome analysis of non-virulent D74 and virulent Nagasaki Haemophilus parasuis isolates - (Peer Reviewed Journal)
Nicholson, T.L., Brunelle, B.W., Bayles, D.O., Alt, D.P., Shore, S. 2018. Comparative genomic and methylome analysis of non-virulent D74 and virulent Nagasaki Haemophilus parasuis isolates. PLoS One. 13(11):e0205700. https://doi.org/10.1371/journal.pone.0205700.

Generation and evaluation of a Haemophilus parasuis capsule mutant - (Abstract Only)
Brockmeier, S., Hau, S., Eberle, K.C., Wang, J., Peters, S.E., Tucker, A.W., Maskell, D.J. 2018. Generation and evaluation of a Haemophilus parasuis capsule mutant. MedVet Pathogens 2018 5th Prato Conference on Animnal Bacterial Pathogens, October 8-11, 2018, Prato, Italy. Abstract No. 29.

Immunoproteomic detection and in vivo screening of protein targets for control of Glasser's disease in pigs - (Abstract Only)
Hau, S.J., Eberle, K.C., Nally, J.E., Lippolis, J.D., Mou, K.T., Brockmeier, S. 2018. Immunoproteomic detection and in vivo screening of protein targets for control of Glasser's disease in pigs. MedVet Pathogens 2018 5th Prato Conference on Animnal Bacterial Pathogens, October 8-11, 2018, Prato, Italy. Abstract No. 48.

Antimicrobial resistance distribution among methicillin resistant Staphylococcus aureus sequence type (ST) 5 isolates from health care and agricultural sources - (Peer Reviewed Journal)
Hau, S.J., Haan, J.S., Davies, P.R., Frana, T., Nicholson, T.L. 2018. Antimicrobial resistance distribution differs among methicillin resistant Staphylococcus aureus sequence type (ST) 5 isolates from health care and agricultural sources. Frontiers in Microbiology. 9:2102. https://doi.org/10.3389/fmicb.2018.02102.

Single nucleotide polymorphism analysis indicates genetic distinction and reduced diversity of agricultural methicillin resistant Staphylococcus aureus (MRSA) ST5 isolates compared to clinical MRSA ST5 isolates - (Peer Reviewed Journal)
Hau, S.J., Allué-Guardia, A., Rusconi, B., Haan, J.S., Davies, P.R., Frana, T.S., Eppinger, M., Nicholson, T.L. 2018. Single nucleotide polymorphism analysis indicates genetic distinction and reduced diversity of swine-associated methicillin resistant Staphylococcus aureus (MRSA) ST5 isolates compared to clinical MRSA ST5 isolates. Frontiers in Microbiology. 9:2078. https://doi.org/10.3389/fmicb.2018.02078.

Blood or serum exposure induce global transcriptional changes, altered antigenic profile, and increased cytotoxicity by classical Bordetellae - (Peer Reviewed Journal)
Gestal, M.C., Rivera, I., Howard, L.K., Dewan, K.K., Soumana, I.H., Dedloff, M., Nicholson, T.L., Linz, B., Harvill, E.T. 2018. Blood or serum exposure induce global transcriptional changes, altered antigenic profile, and increased cytotoxicity by classical Bordetellae. Frontiers in Microbiology. 9:1969. https://doi.org/10.3389/fmicb.2018.01969.

Comparative genomic and virulence analysis of Streptococcus suis isolates - (Abstract Only)

Bordetella bronchiseptica and porcine respiratory disease complex (PRDC) - (Book / Chapter)

Genotypic differences between LA-MRSA ST5 and MRSA ST5 from humans with no swine contact - (Proceedings)
Hau, S.J., Allué-Guardia, A., Rusconi, B., Sun, J., Davies, P.R., Frana, T.S., Eppinger, M., Nicholson, T.L. 2018. Genotypic differences between LA-MRSA ST5 and MRSA ST5 from humans with no swine contact. In: Proceedings of the 49th Annual Meeting of the American Association of Swine Veterinarians, March 3-6, 2018, San Diego, California. p. 57-60.

Interferon alpha inhibits viral replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine - (Peer Reviewed Journal)
Brockmeier, S.L., Loving, C.L., Eberle, K.C., Hau, S.J., Buckley, A., Van Geelen, A., Montiel, N.A., Nicholson, T., Lager, K.M. 2017. Interferon alpha inhibits replication of a live-attenuated porcine reproductive and respiratory syndrome virus vaccine preventing development of an adaptive immune response in swine. Veterinary Microbiology. 212:48-51.

Adherence capacity of methicillin resistant Staphylococcus aureus sequence type (ST) 5 isolates from health care and agricultural sources - (Peer Reviewed Journal)
Hau, S.J., Kellner, S., Eberle, K.C., Waack, U., Brockmeier, S.L., Haan, J.S., Davies, P.R., Frana, T., Nicholson, T.L. 2018. Methicillin-resistant Staphylococcus aureus sequence type (ST) 5 isolates from health care and agricultural sources adhere equivalently to human keratinocytes. Applied Environmental Microbiology. 84(2):e02073-17. https://doi.org/10.1128/AEM.02073-17.

Draft genome sequences of 64 swine associated LA-MRSA ST5 isolates from the USA - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Frana, T.S., Nicholson, T.L. 2017. Draft genome sequences of 63 swine-associated methicillin-resistant Staphylococcus aureus sequence type 5 isolates from the United States. Genome Announcements. 5(44):e01081-17.

Vaccine development for protection against systemic infections with Streptococcus suis and Haemophilus parasuis in swine - (Abstract Only)

Draft genome sequences of 50 MRSA ST5 isolates obtained from a U.S. hospital - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Nicholson, T.L. 2017. Draft genome sequences of 50 methicillin-resistant Staphylococcus aureus sequence type 5 isolates obtained from a U.S. hospital. Genome Announcements. 5(44):e01083-17.

Draft genome sequences of 1 MSSA and 7 MRSA ST5 isolates obtained from California - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Nicholson, T.L. 2017. Draft genome sequences of one methicillin-sensitive and seven methicillin-resistant Staphylococcus aureus sequence type 5 Isolates obtained in California. Genome Announcements. 5(42):e01084-17.

Draft genome sequences of 14 swine associated LA-MRSA ST398 isolates from the U.S. - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Frana, T.S., Nicholson, T.L. 2017. Draft genome sequences of 14 livestock-associated methicillin-resistant Staphylococcus aureus sequence type 398 isolates from swine farms in the United States. Genome Announcements. 5(44):e01082-17.

Draft genome sequences of 9 LA-MRSA ST5 isolates from humans with long term swine contact - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Davies, P.R., Haan, J.S., Nicholson, T.L. 2017. Draft genome sequences of nine livestock-associated methicillin-resistant Staphylococcus aureus sequence type 5 isolates from humans with long-term swine contact. Genome Announcements. 5(41):e01079-17.

Draft genome sequences of 9 LA-MRSA ST5 isolates obtained from humans after short term swine contact - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Frana, T.S., Nicholson, T.L. 2017. Draft genome sequences of nine livestock-associated methicillin-resistant Staphylococcus aureus sequence type 5 isolates obtained from humans after short-term swine contact. Genome Announcements. 5(41):e01080-17.

Bordetellosis - (Book / Chapter)

Complete genome sequence of livestock associated methicillin resistant Staphylococcus aureus ST398 isolated from swine in USA - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Frana, T.S., Nicholson, T.L. 2017. Complete genome sequence of livestock-associated methicillin-resistant Staphylococcus aureus sequence type 398 isolated from swine in the United States. Genome Announcements. 5(32):pii: e00790-17. https://doi.org/10.1128/genomeA.00790-17.

Complete genome sequence of a swine associated LA-MRSA ST5 isolate from the USA - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Frana, T.S., Nicholson, T.L. 2017. Complete genome sequence of a swine associated LA-MRSA ST5 isolate from the USA. Genome Announcements. 5(32):e00791-17. https://doi.org/10.1128%2FgenomeA.00791-17.

The Bordetella Bps polysaccharide is required for biofilm formation and persistence in the lower respiratory tract of swine - (Peer Reviewed Journal)
Nicholson, T.L., Brockmeier, S.L., Sukumar, N., Paharik, A.E., Lister, J.L., Horswill, A.R., Kehrli, M.E. Jr., Loving, C.L., Shore, S.M., Deora, R. 2017. The Bordetella Bps polysaccharide is required for biofilm formation and enhances survival in the lower respiratory tract of swine. Infection and Immunity. 00261-17. doi:10.1128/IAI.00261-17.

Comparative genomic and transcriptional analysis of virulent and non-virulent Haemophilus parasuis isolates - (Abstract Only)

Potential use of outer membrane proteins as subunit vaccines against Haemophilus parasuis - (Abstract Only)

The quest for cross protective factors of Haemophilus parasuis using 2-D gel electrophoresis - (Abstract Only)

Effect of in-feed versus injected oxytetracycline on piglet nasal and tonsil microbiota - (Abstract Only)

Draft genome sequences of 14 Staphylococcus aureus ST5 isolates from California, USA - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Nicholson, T.L. 2017. Draft genome sequences of 14 Staphylococcus aureus sequence type 5 isolates from California, USA. Genome Announcements. 5(13):e00098-17. doi.org/10.1128/genomeA.00098-17.

Complete genome sequences of two Staphylococcus aureus ST5 isolates from California, USA - (Peer Reviewed Journal)
Hau, S.J., Bayles, D.O., Alt, D.P., Nicholson, T.L. 2017. Complete genome sequences of two Staphylococcus aureus sequence type 5 isolates from California, USA. Genome Announcements. 5:e00099-17. doi.org/10.1128/genomeA.00099-17.