Research Project: Non-antibiotic Strategies to Control Enteric Diseases of Poultry

Location: Animal Biosciences & Biotechnology Laboratory

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Immunization with pooled antigens for Clostridium perfringens conferred partial protection against experimental necrotic enteritis in broiler chickens - (Peer Reviewed Journal)
Yuan, B., Sun, Z., Lu, M., Lillehoj, H.S., Lee, Y., Liu, L., Yan, X., Li, C.Z. 2022. Immunization with pooled antigens for Clostridium perfringens conferred partial protection against experimental necrotic enteritis in broiler chickens. Vaccines. 10(6):979. https://doi.org/10.3390/vaccines10060979.

Clostridium perfringens-induced host-pathogen transcriptional changes in the small intestine of broiler chickens - (Peer Reviewed Journal)
Qu, G., Xu, Z., Tuo, W., Li, C.Z., Wan, G., Lillehoj, H.S., Gong, H., Huang, J., Tian, G., Li, S., Liu, Y., Liu, L. 2021. Clostridium perfringens-induced host-pathogen transcriptional changes in the small intestine of broiler chickens. Frontiers in Veterinary Science. https://doi.org/10.1016/j.vetpar.2021.109642.

Cytokine-cytokine receptor interactions in the highly pathogenic avian influenza H5N1 virus-infected lungs of genetically disparate Ri chicken lines - (Peer Reviewed Journal)
Vu, T.H., Hong, Y., Truong, A.D., Lee, J., Lee, S., Song, K., Cha, J., Dang, V.D., Tran, H., Lillehoj, H.S., Hong, Y.H. 2022. Cytokine-cytokine receptor interactions in the highly pathogenic avian influenza H5N1 virus-infected lungs of genetically disparate Ri chicken lines. Journal of Animal Bioscience. https://doi.org/10.5713/ab.21.0420.

Clostridium perfringens-Induced Host-Pathogen Transcriptional Changes in the Small Intestine of Broiler Chickens - (Peer Reviewed Journal)
Lu, M., Yuan, B., Yan, X., Sun, Z., Lillehoj, H.S., Lee, Y., Li, C.Z. 2021. Clostridium perfringens-Induced Host-Pathogen Transcriptional Changes in the Small Intestine of Broiler Chickens. Pathogens. 10(12):1607. https://doi.org/10.3390/pathogens10121607.

Influenza a pathway analysis of highly pathogenic avian influenza virus (H5N1) infection in genetically disparate Ri chicken lines - (Peer Reviewed Journal)
Hong, Y., Lee, J., Vu, T.H., Lee, S., Heo, J., Truong, A.D., Lillehoj, H.S., Hong, Y.H. 2022. Influenza a pathway analysis of highly pathogenic avian influenza virus (H5N1) infection in genetically disparate Ri chicken lines. Veterinary Immunology and Immunopathology. 246:110404. https://doi.org/10.1016/j.vetimm.2022.110404.

Development of antigen-capture ELISA to analyze the immunological functions of chicken interferon-kappa using specific monoclonal antibodies - (Peer Reviewed Journal)
Lee, Y., Lu, M., Lillehoj, H.S. 2021. Development of antigen-capture ELISA to analyze the immunological functions of chicken interferon-kappa using specific monoclonal antibodies. Developmental and Comparative Immunology. https://doi.org/10.1016/j.dci.2021.104204.

Bacillus subtilis expressing chicken NK-2 peptide protects against Eimeria acervulina challenge infection in broiler chickens - (Peer Reviewed Journal)
Wickramasuriya, S.S., Park, I., Van Oosterwijk, J., Gay, C.G., Przybyszwek, C., Lillehoj, H.S. 2021. Bacillus subtilis expressing chicken NK-2 peptide protects against Eimeria acervulina challenge infection in broiler chickens. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2021.684818.

Immunomodulatory effects of poly (I:C)-stimulated exosomes derived from chicken macrophages - (Peer Reviewed Journal)
Hong, Y., Lee, J., Vu, T.H., Lillehoj, H.S., Hong, Y.H. 2021. Immunomodulatory effects of poly (I:C)-stimulated exosomes derived from chicken macrophages. International Journal of Molecular Sciences. https://doi.org/10.1016/j.psj.2021.101247.

Impacts of increasing challenge of E. maxima on gut integrity and gene expression of nutrient transporters in broiler chickens - (Peer Reviewed Journal)
Teng, P., Choi, J., Tompkin, Y.H., Lillehoj, H.S., Kim, W. 2021. Impacts of increasing challenge of E. maxima on gut integrity and gene expression of nutrient transporters in broiler chickens. International Journal of Parasitology. https://doi.org/10.1186/s13567-021-00949-3.

Effects of dietary maltol on innate immunity, gut health and growth performance of broiler chicken challenged with Eimeria maxima - (Peer Reviewed Journal)
Park, I., Goo, D., Nam, H., Wickramasuriya, S., Lee, K., Zimmerman, N.P., Smith, A.H., Rehberger, T.G., Lillehoj, H.S. 2021. Effects of dietary maltol on innate immunity, gut health and growth performance of broiler chicken challenged with Eimeria maxima. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2021.667425.

Alternatives to antibiotics: A symposium on the challenges and solutions for animal health and production - (Peer Reviewed Journal)
Callaway, T.R., Lillehoj, H.S., Chuanchuen, R., Gay, C.G. 2021. Alternatives to antibiotics: A symposium on the challenges and solutions for animal health and production. Antibiotics. https://doi.org/10.3390/antibiotics10050471.

Comparison of the pathogenicity of five clostridium perfringens isolates using an eimeria maxima coinfection necrotic enteritis disease model in commercial broiler chickens - (Peer Reviewed Journal)
Liu, L., Yan, X., Lillehoj, H.S., Sun, Z., Zhao, H., Xianyu, Z., Lee, Y., Melville, S., Gu, C., Li, C.Z. 2021. Comparison of the pathogenicity of five clostridium perfringens isolates using an eimeria maxima coinfection necrotic enteritis disease model in commercial broiler chickens. Avian Diseases. https://doi.org/10.1637/aviandiseases-D-19-00098.

Hypertrophy of adipose tissues in quail embryos by in ovo all-trans retinoic acid injection - (Peer Reviewed Journal)
Kim, D., Lee, J., Kim, S., Lillehoj, H.S., Lee, K. 2021. Hypertrophy of adipose tissues in quail embryos by in ovo all-trans retinoic acid injection. Nutrients. https://doi.org/10.3389/fphys.2021.681562.

First report on detection of necrotic enteritis (NE) B-like toxin in biological samples from NE-afflicted chickens by capture enzyme-linked immunosorbent assay - (Peer Reviewed Journal)
Lee, K., Lillehoj, H.S., Kim, W., Park, I., Li, C.Z., Lu, M., Hofacre, C.L. 2021. First report on detection of necrotic enteritis (NE) B-like toxin in biological samples from NE-afflicted chickens by capture enzyme-linked immunosorbent assay. Poultry Science. https://doi.org/10.1016/j.psj.2021.101190.

Development of antigen sandwich ELISA to detect interferon-alpha (IFN-a) using monoclonal antibodies in chicken - (Abstract Only)

Unveiling the immunological attributes of chicken tumor necrosis factor-a (TNF-a) using new sets of monoclonal antibodies specific for poultry TNF-a - (Abstract Only)

Avian-specific immune reagents and immunoassays to analyze poultry immune response - (Abstract Only)

Characterization of immunological properties of chicken chemokine CC motif ligand 5 using new monoclonal antibodies - (Peer Reviewed Journal)
Lu, M., Panebra, A., Kim, K., Lillehoj, H.S. 2021. Characterization of immunological properties of chicken chemokine CC motif ligand 5 using new monoclonal antibodies. Developmental and Comparative Immunology. https://doi.org/10.1016/j.dci.2021.104023.

Recombinant Bacillus subtilis carrying NK-lysin gene as an effective mucosal delivery strategy to prevent against avian coccidiosis - (Abstract Only)

Effect of dietary maltol as postbiotics on innate immunity, gut health and growth performance of broiler chickens infected with Eimeria maxima - (Abstract Only)

Unveiling the immunological attributes of chicken tumor necrosis factor-a via the development of specific monoclonal antibodies - (Abstract Only)

Development of antigen-capture ELISA to analyze the immunological functions of chicken interferon-kappa - (Abstract Only)

Exosomal miRNA profiling from H5N1 avian influenza virus-infected chickens - (Peer Reviewed Journal)
Hong, Y., Troung, A., Lee, J., Vu, T., Lee, S., Song, K., Lillehoj, H.S., Hong, Y. 2021. Exosomal miRNA profiling from H5N1 avian influenza virus-infected chickens. Scientific Reports. https://doi.org/10.1186/s13567-021-00892-3.

Exosomes of lipopolysaccharide-stimulated chicken macrophages modulate immune response through the MyD88/NF-kB signaling pathway - (Peer Reviewed Journal)
Hong, Y., Lee, J., Vu, T., Lee, S., Lillehoj, H.S., Hong, Y. 2020. Exosomes of lipopolysaccharide-stimulated chicken macrophages modulate immune response through the MyD88/NF-kB signaling pathway. Developmental and Comparative Immunology. https://doi.org/10.1016/j.dci.2020.103908.

Development of antigen capture ELISA to detect interferon-alpha (IFN-a) using monoclonal antibodies in chicken - (Peer Reviewed Journal)
Lee, Y., Kim, W., Lee, S., Lillehoj, H.S. 2020. Development of antigen capture ELISA to detect interferon-alpha (IFN-a) using monoclonal antibodies in chicken. Poultry Science. https://doi.org/10.1016/j.vetimm.2020.110124.

3, 3'-Diindolylmethane attenuates Riemerella anatipestifer infection by suppressing inflammatory responses - (Peer Reviewed Journal)
Fernandez-Colorado, C., Cammayo, P., Flores, R., Binh,, N., Kim, W., Kim, S., Lillehoj, H.S., Min, W. 2020. 3, 3'-Diindolylmethane attenuates Riemerella anatipestifer infection by suppressing inflammatory responses. Frontiers in Microbiology. https://doi.org/10.1371/journal.pone.0242198.

Development and characterization of novel mouse monoclonal antibodies against chicken chemokine CC motif ligand 4 - (Peer Reviewed Journal)
Lu, M., Kim, W., Lillehoj, H.S., Li, C.Z. 2020. Development and characterization of novel mouse monoclonal antibodies against chicken chemokine CC motif ligand 4. Veterinary Immunology and Immunopathology. https://doi.org/10.1016/j.vetimm.2020.110091.

Identification of enterobacteria in viscera of pigs afflicted with porcine reproductive and respiratory syndrome and other viral co-infections - (Peer Reviewed Journal)
Zhao, G., Zhang, L., Li, C.Z., Zhao, J., Liu, N., Li, Y., Wang, J., Liu, L. 2020. Identification of enterobacteria in viscera of pigs afflicted with porcine reproductive and respiratory syndrome and other viral co-infections. Microbial Pathogenesis. 147:10385.

Molecular identification and characterization of a novel chicken leukocyte immunoglobulin-like receptor A5 - (Peer Reviewed Journal)
Truong, A., Hong, Y., Nguyen, H., Nguyen, C., Chu, N., Tran, H., Dang, H., Lillehoj, H.S., Hong, Y. 2020. Molecular identification and characterization of a novel chicken leukocyte immunoglobulin-like receptor A5. British Poultry Science. https://doi.org/10.1080/00071668.2020.1812524.

Role of Clostridium perfringens necrotic enteritis (NE) B-like toxin in NE pathogenesis - (Review Article)
Lee, K., Lillehoj, H.S. 2020. Role of Clostridium perfringens necrotic enteritis (NE) B-like toxin in NE pathogenesis. Avian Pathology. https://doi.org/10.1637/0005-2086-64.4.490.

Detection of Necrotic Enteritis B-like toxin secreted by Clostridium perfringens using capture Enzyme-Linked Immunosorbent Assay - (Peer Reviewed Journal)
Lee, K., Kim, W., Li, C.Z., Lillehoj, H.S. 2020. Detection of Necrotic Enteritis B-like toxin secreted by Clostridium perfringens using capture Enzyme-Linked Immunosorbent Assay. Avian Diseases. 64(4):490-495. https://doi.org/10.1637/0005-2086-64.4.490.

A novel a/ß hydrolase domain protein derived from Haemonchus Contortus acts at the parasite-host interface - (Peer Reviewed Journal)
Lu, M., Tian, X., Tian, A., Li, C.Z., Yan, R., Xu, L., Song, X., Li, X. 2020. A novel a/ß hydrolase domain protein derived from Haemonchus Contortus acts at the parasite-host interface. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2020.01388.

Unveiling the immunomodulatory properties of Haemonchus contortus adhesion regulating molecule 1 in the parasite-host interactions - (Peer Reviewed Journal)
Lu, M., Tian, X., Zhang, Y., Aimulajiang, K., Wang, W., Ehsan, M., Li, C.Z., Yan, R., Xu, L., Lu, X. 2020. Unveiling the immunomodulatory properties of Haemonchus contortus adhesion regulating molecule 1 in the parasite-host interactions. Parasites & Vectors. https://doi.org/10.1186/s13071-020-04297-7.

Effects of Eimeria maxima and Clostridium perfringens infections on cecal microbiome components and correlation with body weight in broiler chickens - (Peer Reviewed Journal)
Lu, M., Li, R., Zhao, H., Yan, X., Lillehoj, H.S., Sun, Z., Oh, S., Wang, Y., Li, C.Z. 2020. Effects of Eimeria maxima and Clostridium perfringens infections on cecal microbiome components and correlation with body weight in broiler chickens. Research in Veterinary Science. https://doi.org/10.1016/j.rvsc.2020.05.013.

Immunomodulatory effects of avian ß-defensin 5 in chicken macrophage cell line - (Peer Reviewed Journal)
Hong, J., Lee, J., Vu, T., Lillehoj, H.S., Hong, Y. 2020. Immunomodulatory effects of avian ß-defensin 5 in chicken macrophage cell line. Research in Veterinary Science. https://doi.org/10.1016/j.rvsc.2020.06.002.

Characterization of monoclonal antibodies and development of sandwich ELISA detecting chicken interleukin-7 - (Peer Reviewed Journal)
Panebra, A., Kim, W., Hong, Y., Lillehoj, H.S. 2021. Characterization of monoclonal antibodies and development of sandwich ELISA detecting chicken interleukin-7. Research in Veterinary Science. https://doi.org/10.1016/j.psj.2020.12.037.

"Developing next-generation antibiotic alternatives to antibiotics" - (Abstract Only)

Proteomic analysis revealed T cell hyporesponsiveness induced by Haemonchus contortus excretory and secretory proteins - (Peer Reviewed Journal)
Lu, M., Tian, X., Yang, Z., Wang, W., Tian, A., Li, C.Z., Yan, R., Xu, L., Song, X., Li, X. 2020. Proteomic analysis revealed T cell hyporesponsiveness induced by Haemonchus contortus excretory and secretory proteins. Veterinary Research. https://doi.org/10.1186/s13567-020-00790-0.

Dietary supplementation with magnolia bark extract alters chicken intestinal metabolite levels - (Peer Reviewed Journal)
Park, I., Lillehoj, E., Lillehoj, H.S. 2020. Dietary supplementation with magnolia bark extract alters chicken intestinal metabolite levels. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2020.00157.

Immunization with a subunit of housekeeping proteins provide partial protection against experimental necrotic enteritis in broiler chickens - (Abstract Only)

Dietary supplementation with Bacillus subtilis direct-fed microbials alters chicken intestinal metabolite levels - (Peer Reviewed Journal)
Park, I., Zimmerman, N.P., Smith, A.H., Rehberger, T.G., Lillehoj, E.P., Lillehoj, H.S. 2020. Dietary supplementation with Bacillus subtilis direct-fed microbials alters chicken intestinal metabolite levels. Scientific Reports. https://doi.org/10.3389/fvets.2020.00123.

Putting antimicrobial resistance in the corner - (Review Article)
Li, C.Z., Lu, M. 2020. Putting antimicrobial resistance in the corner. Nature Food. https://doi.org/10.1038/s43016-020-0034-9.

Haemonchus contortus transthyretin domain - containing protein (HcTTR): A promising vaccine candidate against Haemonchus contortus infection - (Peer Reviewed Journal)
Tian, X., Lu, M., Jia, C., Bu, Y., Aimulajiang, K., Zhang, Y., Li, C.Z., Yan, R., Xu, L., Li, X. 2020. Haemonchus contortus transthyretin domain - containing protein (HcTTR): A promising vaccine candidate against Haemonchus contortus infection. Veterinary Parasitology. https://doi.org/10.1016/j.vetpar.2020.109045.

Development and characterization of monoclonal antibodies for chicken interleukin-13 and their neutralizing effect in chicken primary monocytes - (Peer Reviewed Journal)
Chaudharia, A., Kim, W., Lillehoj, H.S. 2020. Development and characterization of monoclonal antibodies for chicken interleukin-13 and their neutralizing effect in chicken primary monocytes. Veterinary Immunology and Immunopathology. https://doi.org/10.1016/j.psj.2019.10.023.

Beneficial effects of dietary supplementation of Bacillus strains on growth performance and gut health in chicken with mixed coccidiosis infection - (Peer Reviewed Journal)
Chaudhari, A., Lee, Y., Lillehoj, H.S. 2020. Beneficial effects of dietary supplementation of Bacillus strains on growth performance and gut health in chicken with mixed coccidiosis infection. Probiotics and Antimicrobial Proteins. https://doi.org/10.1016/j.psj.2019.10.023.

"Development and characterization of novel mouse monoclonal antibodies against chicken chemokine CC motif ligand 4" - (Abstract Only)

"Detection of necrotic enteritis (NE) B-like toxin in biological samples of NE-afflicted chickens by capture Enzyme-Linked Immunosorbent Assay" - (Abstract Only)

"Differential detection of interleukin-12 and -23 proteins in chicken coccidiosis" - (Abstract Only)

"Dietary supplementation with Bacillus subtilis direct-fed microbials alters chicken intestinal metabolite levels" - (Abstract Only)

"Chicken avian beta-defensin 8 modulates immune response via mitogen-activated protein kinase signaling pathways in a chicken macrophage cell line" - (Peer Reviewed Journal)
Hong, Y., Pham, T.T., Lee, J., Lillehoj, H.S., Hong, Y. 2020. "Chicken avian beta-defensin 8 modulates immune response via mitogen-activated protein kinase signaling pathways in a chicken macrophage cell line". BMC Veterinary Research. https://doi.org/10.21203/rs.2.17721/v2.

Strategic priorities for research on antibiotic alternatives in animal agriculture-Results from an expert workshop - (Peer Reviewed Journal)
Kurt, T., Wong, N., Fowler, N., Gay, C.G., Lillehoj, H.S., Plummer, P., Scott, M., Hoelzer, K. 2019. Strategic priorities for research on antibiotic alternatives in animal agriculture-Results from an expert workshop. Frontiers in Veterinary Science. https://doi.org/10.3389/fvets.2019.00429.

"Involvement of T cell immunity in Avian Coccidiosis" - (Peer Reviewed Journal)
Kim, W., Chaudhari, A., Lillehoj, H.S. 2019. "Involvement of T cell immunity in Avian Coccidiosis". Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02732.

Dietary antibiotic growth promoters down-regulate inflammatory cytokine expression in chickens challenged with LPS or co-infected with Eimeria maxima and Clostridium perfringens - (Peer Reviewed Journal)
Oh, S., Lillehoj, H.S., Lee, Y., Bravo, D., Lillehoj, E.P. 2019. Dietary antibiotic growth promoters down-regulate inflammatory cytokine expression in chickens challenged with LPS or co-infected with Eimeria maxima and Clostridium perfringens. PLoS One. https://doi.org/10.3389/fvets.2019.00420.

Host immunity in coccidiosis - (Book / Chapter)
Kim, W., Atul, C., Lillehoj, H.S. 2019. Host immunity in coccidiosis. CRC Press. https://doi.org/10.3389/fimmu.2019.02732.

Phytochemicals as antibiotic alternatives: mechanism of action of enhanced anti-oxidant and growth promoting effect of dietary phytochemicals - (Abstract Only)

Immunization with subunits of important virulence factors produced by virulent Clostridium perfringens strains conferred partial protection against necrotic enteritis in broiler chickens - (Abstract Only)

The effects of dietary Bacillus subtilis supplementation, as an alternative to antibiotics, on growth performance, intestinal immunity, and epithelial barrier integrity in broiler chickens infected with Eimeria maxima - (Peer Reviewed Journal)
Park, I., Lee, Y., Zimmerman, N., Smith, A., Rehberger, T., Lillehoj, H.S. 2020. The effects of dietary Bacillus subtilis supplementation, as an alternative to antibiotics, on growth performance, intestinal immunity, and epithelial barrier integrity in broiler chickens infected with Eimeria maxima. Poultry Science. https://doi.org/10.1016/j.psj.2019.12.002.

The effects of dietary Baccillus subtilis supplementation, as an alternative to antibiotics, on growth performance, intestinal immunity, and epithelial barrier integrity in broiler chickens infected with Eimeria maxima - (Peer Reviewed Journal)
Park, I., Lee, Y., Goo, D., Zimmerman, N., Smith, A., Rehberger, T., Lillehoj, H.S. 2020. The effects of dietary Baccillus subtilis supplementation, as an alternative to antibiotics, on growth performance, intestinal immunity, and epithelial barrier integrity in broiler chickens infected with Eimeria maxima. Poultry Science. https://doi.org/10.1016/j.psj.2019.12.002.

"Small molecular weight metabolites regulating growth and immunity as postbiotic antibiotic alternatives" - (Abstract Only)

"Host defense peptides with anti-microbial and immunomodulatory activities as antibiotic alternatives" - (Abstract Only)

ANTIBIOTICS, GERMS AND ANTIBIOTIC ALTERNATIVES - (Abstract Only)

"Novel hyperimmune egg yolk IgY antibodies developed against protective antigens of Eimeria sp and Clostridium perfringens protect against coccidiosis and necrotic enteritis" - (Abstract Only)

Submission of genome sequences of Clostridium perfringens strain LLY_Tpel17 into genBank database - (Database / Dataset)

Characterization and functional analysis of novel chicken leukocyte immunoglobulin-like receptor subfamily B member 4 and 5 - (Peer Reviewed Journal)
Truong, A., Hong, Y., Tran, H., Dang, H., Nguyen, V., Pham, T., Lillehoj, H.S., Hong, Y. 2019. Characterization and functional analysis of novel chicken leukocyte immunoglobulin-like receptor subfamily B member 4 and 5. Poultry Science. https://doi.org/10.3382/ps/pez442.

Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens - (Peer Reviewed Journal)
Li, C.Z., Yan, X., Lillehoj, H.S., Gu, C., Sun, Z., Oh, S., Lee, Y., Xianyu, Z., Zhao, H., Liu, L. 2019. Eimeria maxima-induced transcriptional changes in the cecal mucosa of broiler chickens. Parasites & Vectors. https://doi.org/10.1186/s13071-019-3534-4.

Eimeria maxima-induced transcriptional changes in the chicken intestine - (Peer Reviewed Journal)
Li, C.Z., Yan, X., Lillehoj, H.S., Gu, C., Sun, Z., Oh, S., Lee, Y., Xianyu, Z., Zhao, H., Liu, L. 2019. Eimeria maxima-induced transcriptional changes in the chicken intestine. Frontiers in Immunology. 12:285. https://doi.org/10.1186/s13071-019-3534-4.

The effects of direct-fed microbial supplementation, as an alternative to antibiotics, on growth performance, intestinal immunity, and epithelial barrier integrity in broiler chickens infected with Eimeria maxima - (Abstract Only)

Eimeria tenella Elongation Factor-1a (EF-1a) co-administered with chicken IL-7 (chIL-7) DNA vaccine emulsified in Montanide Gel 01 adjuvant enhanced immune response to E. acervulina infection - (Abstract Only)

Development of sandwich ELISA for differential detection of chicken heterodimeric cytokines, interleukin-12 and -23 - (Abstract Only)

Development of poultry-specific immune reagents - (Abstract Only)

Development of novel monoclonal antibodies against chicken interleukin- 4 and alternative activation of macrophage-like cells in chickens - (Abstract Only)

The effect of dietary supplementation of several Bacillus strains on growth performance and gut health in mixed coccidiosis infection in chickens - (Abstract Only)

Mechanism of action of antibiotic growth promoters: Alteration of immune response and intestinal microbiome - (Abstract Only)

Clostridium perfringens induces IL-10 secretion in chicken intestinal epithelial cells and necrotic enteritis broiler chicken - (Abstract Only)

Indole treatment alleviates intestinal tissue damage induced by chicken coccidiosis through activation of the aryl hydrocarbon receptor - (Peer Reviewed Journal)
Kim, W., Lillehoj, H.S., Min, W. 2019. Indole treatment alleviates intestinal tissue damage induced by chicken coccidiosis through activation of the aryl hydrocarbon receptor. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.00560.

"Phytochemical modulation of poultry gut microbiota" - (Abstract Only)

Vaccination with Eimeria tenella elongation factor-1a with IL-7 DNA vaccine protect broiler chickens against Coccidiosis - (Peer Reviewed Journal)
Panebra, A., Lillehoj, H.S. 2019. Vaccination with Eimeria tenella elongation factor-1a with IL-7 DNA vaccine protect broiler chickens against Coccidiosis. Avian Diseases. 63:342-350. https://doi.org/10.1637/11976-092418-Reg.1.

The transcriptional changes in chicken intestines in response to C. perfringens infection - (Abstract Only)

Expression and characterization of chicken perforin and granzyme a - (Abstract Only)

"Serum concentration of acute phase proteins and cytokines in vaccinated pigs challenged against food-and-mouth disease virus serotype O" - (Peer Reviewed Journal)
Lee, K., Kim, D., Lee, K., Lillehoj, H.S., Park, J. 2019. "Serum concentration of acute phase proteins and cytokines in vaccinated pigs challenged against food-and-mouth disease virus serotype O". Revista Brasileira de Zootecnia. https://doi.org/10.1590/rbz4820180190.

Differential detection of chicken heterodimeric cytokines, interleukin-12 and -23 using specific monoclonal antibodies to their subunits - (Abstract Only)

Development of poultry immune reagent - (Abstract Only)

"Allium hookeri leaf as a fed supplement influences gut microbiome realted to growth performance in young broiler chickens" - (Peer Reviewed Journal)
Lee, S., Bang, S., Kim, B., Kim, S., Kang, S., Lillehoj, H.S. 2018. "Allium hookeri leaf as a fed supplement influences gut microbiome realted to growth performance in young broiler chickens". Veterinary Microbiology. https://doi.org/10.5713/ajas.18.0571.

Identification of duck liver-expressed antimicrobial peptide 2 (LEAP-2) and characterization of its bactericidal activity - (Peer Reviewed Journal)
Hong, Y., Truong, A., Lee, J., Lee, K., Kim, G., Heo, K., Lillehoj, H.S., Hong, Y. 2018. Identification of duck liver-expressed antimicrobial peptide 2 (LEAP-2) and characterization of its bactericidal activity. Asian-Australasian Journal of Animal Sciences. https://doi.org/10.5713/ajas.18.0571.

"Interleukin-4 (IL-4) regulates alternative activation of macrophages in chickens: a sequential study using novel and specific neutralizing monoclonal antibodies against chicken IL-4" - (Peer Reviewed Journal)
Chaudhari, A., Kim, W., Lillehoj, H.S. 2018. "Interleukin-4 (IL-4) regulates alternative activation of macrophages in chickens: a sequential study using novel and specific neutralizing monoclonal antibodies against chicken IL-4". Developmental and Comparative Immunology. 205:72-82.

"Chicken novel leukocyte immunoglobulin-like receptor subfamilies B1 and B3 are transcriptional regulators of MHC class I genes and signaling pathways" - (Peer Reviewed Journal)
Troung, A.D., Hong, Y., Lee, J., Lee, K., Lillehoj, H.S., Hong, Y. 2019. "Chicken novel leukocyte immunoglobulin-like receptor subfamilies B1 and B3 are transcriptional regulators of MHC class I genes and signaling pathways". Molecular Immunology. 32:614-620.

Pleiotropic anti-infective effects of a small-molecule lipid II binder - (Peer Reviewed Journal)
De Leeuw, E.P., Kee, S., Kwasny, S.M., Opperman, T.J., Lillehoj, H.S. 2018. Pleiotropic anti-infective effects of a small-molecule lipid II binder. Infection and Drug Resistance. 62:381-387.

Growth performance, nutrient availability and immunity responses in broiler chickens fed a commercial blend of phytogenic feed additives - (Peer Reviewed Journal)
Pirgozliev, V., Mansbridge, S., Rose, S., Lillehoj, H.S., Bravo, D. 2019. Growth performance, nutrient availability and immunity responses in broiler chickens fed a commercial blend of phytogenic feed additives. Poultry Science. https://doi.org/10.3382/ps/pey472.

"Interlaeukin-34 regulates Th1 and Th17 cytokine production by activating multiple signaling pathways through CSF-1R in chicken cell lines" - (Peer Reviewed Journal)
Troung, A.D., Hong, Y., Lee, J., Lee, K., Kil, D., Lillehoj, H.S., Hong, Y. 2018. "Interlaeukin-34 regulates Th1 and Th17 cytokine production by activating multiple signaling pathways through CSF-1R in chicken cell lines". Oncotarget. 19(9):2710 E1665.

"Leukocyte Immunoglobulin-like receptors A2 and A6 are expressed in macrophages and modulate cytokine production by activating multiple signaling pathways" - (Peer Reviewed Journal)
Truong, A., Rengaraj, D., Hong, Y., Tran, H., Dang, H., Nguyen, V., Lillehoj, H.S., Hong, Y. 2018. "Leukocyte Immunoglobulin-like receptors A2 and A6 are expressed in macrophages and modulate cytokine production by activating multiple signaling pathways". Journal of Animal Science and Biotechnology. https://doi.org/10.3390/ijms19092710.

Detection of chicken interleukin-10 production in epithelial cells and necrotic enteritis induced by Clostridium perfringens using capture-ELISA - (Peer Reviewed Journal)
Lee, Y., Kim, W., Lee, S., Lillehoj, H.S. 2018. Detection of chicken interleukin-10 production in epithelial cells and necrotic enteritis induced by Clostridium perfringens using capture-ELISA. Veterinary Immunology and Immunopathology. https://doi.org/10.1016/j.vetimm.2018.10.001.

Characterization of intestinal immune response to clostridium perfringens infection in broiler chickens characterization of intestinal immune tesponse to clostridium perfringens infection in broiler chickens - (Peer Reviewed Journal)
Fasina, Y.O., Lillehoj, H.S. 2018. Characterization of intestinal immune response to clostridium perfringens infection in broiler chickens characterization of intestinal immune tesponse to clostridium perfringens infection in broiler chickens. Poultry Science. 98:188-198.

Comparison of pathogenicity of several Clostridium perfringens strains with Eimeria maxima co-infection in inducing necrotic enteritis lesions in broiler chickens - (Abstract Only)
Li, C.Z., Liu, L., Yan, X., Lillehoj, H.S., Sun, Z., Zhao, H., Xianyu, Z., Lee, Y. 2018. Comparison of pathogenicity of several Clostridium perfringens strains with Eimeria maxima co-infection in inducing necrotic enteritis lesions in broiler chickens. Meeting Abstract. P19.

Characterization of clostridium perfringens netB+tpel+ type A atrains isolated from necrotic enteritis-afflicted broiler chickens - (Other)
Li, C.Z., Lillehoj, H.S., Yan, X., Gu, C., Sun, Z., Lee, Y., Zhao, H., Xianyu, Z., Siragusa, G.R., Grant, A. 2018. Characterization of clostridium perfringens netB+tpel+ type A atrains isolated from necrotic enteritis-afflicted broiler chickens. Meeting Abstract. P52.

Immunity, immunomodulation, and antibiotic alternatives to maximize the genetic potential of poultry for growth and disease response - (Peer Reviewed Journal)
Kim, W., Lillehoj, H.S. 2018. Immunity, immunomodulation, and antibiotic alternatives to maximize the genetic potential of poultry for growth and disease response. Animal Feed Science And Technology. 250:41-50.

Down-regulation of common cytokine receptor gamma chain inhibits inflammatory responses in macrophages stimulated with Riemerella anatipestifer - (Peer Reviewed Journal)
Afrin, F., Fernandez, C., Flores, R., Kim, W.H., Jeong, J., Chang, H., Kim, S., Lillehoj, H.S., Ming, W. 2018. Down-regulation of common cytokine receptor gamma chain inhibits inflammatory responses in macrophages stimulated with Riemerella anatipestifer. Developmental and Comparative Immunology. 81:225-234. https://doi.org/10.1016/j.dci.2017.12.009.

Phytochemicals as potential antibiotic alternatives to promote growth and enhance host health - (Other)

Effect of dietary Magnolol/Honokiol on growth performance and anti-oxidant status in broiler chickens infected with experimental necrotic enteritis - (Abstract Only)

Development and characterization of chicken CD127-cpecific antibodies - (Abstract Only)

Dietary supplementation of Allium hookeri improved intestinal immune response on necrotic enteritis of young broiler chickens - (Abstract Only)

"Characterization of the immune reagent chicken IL-16" - (Other)

Characterization of the immune reagent chicken IL-16 - (Abstract Only)

Induction of interleukin-10 expression in chicken intestinal epithelial cells stimulated with Clostridium perfringens - (Other)

Bacillus spp. as direct-fed microbial antibiotic alternatives to enhance poultry growth, immunity and intestinal health - (Peer Reviewed Journal)
Grant, A., Gay, C.G., Lillehoj, H.S. 2018. Bacillus spp. as direct-fed microbial antibiotic alternatives to enhance poultry growth, immunity and intestinal health. Avian Pathology. 2:1-13. https://doi.org/10.1080/03079457.2018.1464117.

Identification of duck IL-4 and its inhibitory effect on IL-17A expression in R. anatipestifer-stimulated splenic lymphocytes - (Peer Reviewed Journal)
Fernandez, C.P., Afrin, F., Flores, R.A., Kim, W.H., Jeong, J., Kim, W., Lillehoj, H.S., Min, W. 2018. Identification of duck IL-4 and its inhibitory effect on IL-17A expression in R. anatipestifer-stimulated splenic lymphocytes. Molecular Immunology. 95:20-29. https://doi.org/10.1016/j.molimm.2018.01.009.

Production and characterization of monoclonal antibodies specific for chicken interleukin-4 - (Abstract Only)

Expression and regulation of avian beta-defensin 8 protein in immune tissues and cell lines of chickens - (Peer Reviewed Journal)
Rengaraj, D., Truong, A., Lillehoj, H.S., Han, J., Hong, Y. 2018. Expression and regulation of avian beta-defensin 8 protein in immune tissues and cell lines of chickens. Asian-Australasian Journal of Animal Sciences. https://doi.org/10.5713/ajas.17.0836.

Submission of nucleotide sequence eimeria tenella elongation factor 1-alpha to genBank database - (Other)

Phytochemicals as potential antibiotic alternatives to promote growth and enhance host health: A report from the Second International Symposium on Alternatives to Antibiotics - (Peer Reviewed Journal)
Lillehoj, H.S., Liu, Y., Calsamiglia, S., Fernandez-Miyakawa, M.E., Chi, F., Cravens, R.L., Oh, S., Gay, C.G. 2018. Phytochemicals as potential antibiotic alternatives to promote growth and enhance host health: A report from the Second International Symposium on Alternatives to Antibiotics. Veterinary Research. https://doi.org/10.1186/s13567-018-0562-6.

Detection of chicken interleukin-23, a heterodimeric cytokine with monoclonal antibodies reacting p19 and p40 subunits - (Abstract Only)

Growth promoting and anti-oxidant effects of magnolia bark extract in chicken uninfected or co-infected with clostridium perfringens and eimeria maxima as an experimental model of necrotic enteritis - (Peer Reviewed Journal)
Oh, S., Gadde, U.D., Bravo, D., Lillehoj, E.P., Lillehoj, H.S. 2018. Growth promoting and anti-oxidant effects of magnolia bark extract in chicken uninfected or co-infected with clostridium perfringens and eimeria maxima as an experimental model of necrotic enteritis. Developmental and Comparative Immunology. 2(4):nzy009.

Immune modulation of host response to coccidiosis and necrotic enteritis to reduce antibiotics - (Abstract Only)

Dietary supplementation of allium hookeri improves intestinal immune response against necrotic enteritis in young broiler chickens - (Peer Reviewed Journal)
Lee, Y., Lee, S., Gadde, U., Oh, S., Lee, S., Lillehoj, H.S. 2021. Dietary supplementation of allium hookeri improves intestinal immune response against necrotic enteritis in young broiler chickens. Poultry Science. https://doi.org/10.3382/ps/pey031.

Down-regulation of inflammatory cytokines by berberine attenuates Riemerella anatipestifer infection in ducks - (Peer Reviewed Journal)
Fernandez, C.P., Afrin, F., Flores, R.A., Kim, W.H., Jeong, J., Kim, S., Chang, H.H., Lillehoj, H.S., Min, W. 2017. Down-regulation of inflammatory cytokines by berberine attenuates Riemerella anatipestifer infection in ducks. Developmental and Comparative Immunology. 77:121-127. https://doi.org/10.1016/j.dci.2017.07.027.

Dataset on characterization of recombinant interleukin-23alpha, IL-12p40 and IL-23 complex protein, which activates JAK-STAT signaling pathway in chicken cell line using immunocytochemical - (Peer Reviewed Journal)
Truong, A.D., Hoang, C., Hong, Y., Lee, J., Lee, K., Lillehoj, H.S., Hong, Y.H. 2017. Dataset on characterization of recombinant interleukin-23alpha, IL-12p40 and IL-23 complex protein, which activates JAK-STAT signaling pathway in chicken cell line using immunocytochemical. Data in Brief. 16:799-805. https://doi.org/10.1016/j.dib.2017.12.008.

Allium hookeri supplementation improves intestinal immune response against necrotic enteritis - (Peer Reviewed Journal)
Lee, Y., Lee, S., Gadde, U.D., Oh, S., Lee, S., Lillehoj, H.S. 2018. Allium hookeri supplementation improves intestinal immune response against necrotic enteritis. Poultry Science. 97(6):1899-1908. https://doi.org/10.3382/ps/pey031.

Immunoproteomic analysis of proteins from unsporulated Oocysts of Eimeria tenella in MALDI TOF/TOF tandem mass spectrometry - (Peer Reviewed Journal)
Li, C.Z., Liu, L., Zhenchao, Z., Shuai, W. 2017. Immunoproteomic analysis of proteins from unsporulated Oocysts of Eimeria tenella in MALDI TOF/TOF tandem mass spectrometry. Parasite. 24:48.

Antibiotic growth promoter-induced changes in the chicken intestine: A metabolomics analysis of virginiamycin and bacitracin methylene disalicylate - (Peer Reviewed Journal)
Gadde, U.D., Oh, S., Lillehoj, H.S., Lillehoj, E.P. 2018. Antibiotic growth promoter-induced changes in the chicken intestine: A metabolomics analysis of virginiamycin and bacitracin methylene disalicylate. Scientific Reports. 8(1):3592. https://doi.org/10.1038/s41598-018-22004-6.

Effects of Varium on expression of intestinal barrier, antioxidant and pro-inflammatory cytokine genes in young broiler chickens with experimental necrotic enteritis - (Abstract Only)

Complete genome sequences of slostridium perfringens strain del1 isolated from necrotic enteritis-afflicted chicken farm - (Peer Reviewed Journal)
Li, C.Z., Yan, X., Lillehoj, H.S. 2018. Complete genome sequences of slostridium perfringens strain del1 isolated from necrotic enteritis-afflicted chicken farm. Gut Pathogens. 9:69. https://doi.org/10.1186/s13099-017-0217-6.

Functional analyses of the interaction of chicken interleukin 23 subunit p19 with IL-12 subunit p40 to form the IL-23 complex - (Peer Reviewed Journal)
Truong, A.D., Hoang, C.T., Hong, Y., Lee, J., Lee, K., Lillehoj, H.S., Hong, Y.H. 2017. Functional analyses of the interaction of chicken interleukin 23 subunit p19 with IL-12 subunit p40 to form the IL-23 complex. Molecular Immunology. 92:54-67. https://doi.org/10.1016/j.molimm.2017.09.019.

Effects of Eimeria maxima and Clostridium perfringens infections on Cecal Microbiome in Broiler Chickens Analyzed by 16S rRNA Sequencing - (Abstract Only)

In ovo vaccines based on recombinant NetB toxin and Montanide IMS adjuvants induced protective immunity against Necrotic Enteritis in chickens - (Abstract Only)

Vaccination with Eimeria tenella Elongation Factor-1alpha Recombinant Protein Induces protective Immunity against E. tenella and E. maxima infections - (Peer Reviewed Journal)
Lin, R.Q., Lillehoj, H.S., Lee, S.K., Oh, .S, Panebra, A., Lillehoj, E.P. 2017. Vaccination with Eimeria tenella Elongation Factor-1a Recombinant Protein Induces Protective Immunity against E. tenella and E. maxima Infections. Veterinary Parasitology. 243:79-84.

Differentially expressed JAK-STAT signaling pathway genes and target microRNAs in the spleen of necrotic enteritis-afflicted chicken lines - (Peer Reviewed Journal)
Truong, A.D., Rengaraj, D., Hong, Y., Hoang, C.T., Hong, Y.H., Lillehoj, H.S. 2017. Differentially expressed JAK-STAT signaling pathway genes and target microRNAs in the spleen of necrotic enteritis-afflicted chicken lines. Research in Veterinary Science. 115:235-243.

Eimeria-induced chicken cNK-2 is an anti-infective host defense peptide and an immunomodulator of host innate immunity - (Abstract Only)

Dietary Bacillus subtilis-based direct-fed microbials alleviate LPS-induced intestinal immunological stress and improve intestinal barrier gene expression in commercial broiler chickens - (Peer Reviewed Journal)
Gadde, U., Oh, S.T., Lee, Y.S., Lillehoj, H.S. 2017. Dietary Bacillus subtilis-based direct-fed microbials alleviate LPS-induced intestinal immunological stress and improve intestinal barrier gene expression in commercial broiler chickens. Research in Veterinary Science. 114:236-243.

The effect of direct-fed microbial supplementation, as an alternative to antibiotics, on growth performance, intestinal immune status and epithelial barrier protein expression in broiler chickens - (Abstract Only)

Dietary Allium hookeri reduces inflammatory response and increases expression of intestinal tight junctions proteins in LPS-induced young broiler chickens - (Abstract Only)

Immunomodulation by chicken NK-Lysin-derived peptide, cNK-2 on chicken macrophages and monocytes - (Abstract Only)

Immunomodulation by chicken NK-lysin-derived peptide, c-NK2 on chicken macrophages and monocytes - (Abstract Only)

Relievable effect of dietary Allium hookeri on LPS-induced intestinal inflammation response in young broiler chickens - (Abstract Only)

Intestinal immune response to chicken Coccidiosis in the context of Th1 and Th17 response - (Abstract Only)

Recent progress in understanding host immune response to Avian Coccidiosis: Th1 and Th17 responses - (Abstract Only)

Recent progress in understanding host immune response to Avian Coccidiosis: Th1 and Th17 responses - (Abstract Only)

Intestinal meta-transcriptome comparison reveals disparate antiviral transcriptional response and its association with Mitochondria in chicken immunity development - (Abstract Only)

The effects of direct-fed microbial supplementation, as alternative to antibiotics, on growth performance, intestinal immune status and epithelial barrier protein expression in broiler chickens - (Abstract Only)

Comparative genomic analysis of full genome sequences of two closely related isolates of Clostridium perfringens reveals regions of genome plasticity with prevention potential - (Other)

Submission of nucleotide sequence chicken IL-7 to genbank database - (Other)

Submission of nucleotide sequence clostridium perfringens alpha-toxin to genbank database - (Other)

Submission of nucleotide sequence clostridium perfringens pyruvate-flavodoxin oxi-reductase to genbank database - (Other)

Submission of nucleotide sequence clostridium perfringens NetB toxin to genbank database - (Other)

Submission of nucleotide sequence clostridium perfringens elongation factor-tu to genbank database - (Other)

Supplementation of direct-fed microbial as an alternative to antibiotic on growth performance, intestinal immune status and epithelial barrier integrity in broiler chicken - (Peer Reviewed Journal)
Gadde, U.D., Lee, Y.S., Oh, S., Lillehoj, H.S. 2017. Supplementation of direct-fed microbial as an alternative to antibiotic on growth performance, intestinal immune status and epithelial barrier integrity in broiler chicken. Probiotics and Antimicrobial Proteins. 10.1007/s12602-017-9275-9.

Draft genome sequences of clostridium perfringens strain LLY_N11, a pathogenic isolate of necrotic enteritis from a healthy chicken - (Peer Reviewed Journal)
Li, C.Z., Yan, X., Lillehoj, H.S. 2017. Draft genome sequences of clostridium perfringens strain LLY_N11, a pathogenic isolate of necrotic enteritis from a healthy chicken. Genome Announcements. 5:44. https://doi.org/10.1128/genomeA.01225-17.

Characterization of virulent netB+/tpeI+ clostridium perfringens strains from necrotic-enteritis-affected broiler chicken farms - (Peer Reviewed Journal)
Gu, C., Lillehoj, H.S., Sun, Z., Lee, Y., Zhao, H., Xiangyu, Z., Yan, X., Lin, S., Liu, L., Li, C.Z. 2019. Characterization of virulent netB+/tpeI+ clostridium perfringens strains from necrotic-enteritis-affected broiler chicken farms. Avian Diseases. https://doi.org/10.1637/11973-092018-Reg.1.

Submission of nucleotide sequence eimeria acervulina profilin to genbank database - (Other)