Research Project: Holistic Tactics to Advance the Microbiological Safety and Quality of the Red Meat Continuum

Location: Meat Safety and Quality

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Development of hydrolysis probe assays for the specific detection of pathogenic Escherichia coli O26, O111, O103, and O121 strains: Multi-country validation study - (Peer Reviewed Journal)

Evaluation of methods for identifying poultry wing rinses with Salmonella concentrations greater than or equal to 10 CFU/mL - (Peer Reviewed Journal)

Impact of intense sanitization procedures on bacterial communities recovered from floor drains in pork processing plants - (Peer Reviewed Journal)
Bosilevac, J.M., Guragain, M., Barkhouse, D.A., Velez, S.E., Katz, T., Lu, G., Wang, R. 2024. Impact of intense sanitization procedures on bacterial communities recovered from floor drains in pork processing plants. Frontiers in Microbiology. 15. Article 1379203. https://doi.org/10.3389/fmicb.2024.1379203.

Proportions and serogroups of enterohemorrhagic Shiga Toxin-producing Escherichia coli in feces of fed and cull beef and cull dairy cattle at harvest - (Peer Reviewed Journal)
Bosilevac, J.M., Katz, T.S., Arthur, T.M., Kalchayanand, N., Wheeler, T.L. 2024. Proportions and serogroups of enterohemorrhagic Shiga Toxin-producing Escherichia coli in feces of fed and cull beef and cull dairy cattle at harvest. Journal of Food Protection. 87(6). Article 100273. https://doi.org/10.1016/j.jfp.2024.100273.

Pathogenomes of Shiga toxin positive and negative Escherichia coli O157:H7 strains TT12A and TT12B: Comprehensive phylogenomic analysis using closed genomes - (Peer Reviewed Journal)
Kalalah, A.A., Koenig, S.S., Feng, P.C., Bosilevac, J.M., Bono, J.L., Eppinger, M. 2024. Pathogenomes of Shiga toxin positive and negative Escherichia coli O157:H7 strains TT12A and TT12B: Comprehensive phylogenomic analysis using closed genomes. Microorganisms. 12(4). Article 699. https://doi.org/10.3390/microorganisms12040699.

Insights into the plasticity and virulence of Escherichia coli serogroup O45 - (Abstract Only)
Kalalah, A.A., Higgins, A., Koenig, S.S., Bosilevac, J.M., Eppinger, M. 2024. Insights into the plasticity and virulence of Escherichia coli serogroup O45 [abstract]. American Society of Microbiology Microbe. No 5914.

Pathogenomes of Shiga toxin positive and negative Escherichia coli O157:H7 strains TT12A and B: comprehensive phylogenomic analysis using closed genomes - (Abstract Only)
Kalalah, A.A., Koenig, S.S., Feng, P.C., Bosilevac, J.M., Bono, J.L., Eppinger, M. 2024. Pathogenomes of Shiga toxin positive and negative Escherichia coli O157:H7 strains TT12A and B: comprehensive phylogenomic analysis using closed genomes [abstract]. American Society for Microbiology. No. 5741.

Validation of a new method of sampling beef manufacturing trimmings for pathogen testing using a manual sampling mitt approach - (Peer Reviewed Journal)
Arthur, T.M., Reno, F.J., Wheeler, T.L. 2024. Validation of a new method of sampling beef manufacturing trimmings for pathogen testing using a manual sampling mitt approach. Journal of Food Protection. 87(3). Article 100233. https://doi.org/10.1016/j.jfp.2024.100233.

Impact of intense sanitization on environmental biofilm communities and the survival of Salmonella enterica at a beef processing plant - (Peer Reviewed Journal)
Wang, R., Guragain, M., Chitlapilly Dass, S., Palanisamy, V., Bosilevac, J.M. 2024. Impact of intense sanitization on environmental biofilm communities and the survival of Salmonella enterica at a beef processing plant. Frontiers in Microbiology. 15. Article 1338600. https://doi.org/10.3389/fmicb.2024.1338600.

Evaluation of peracetic acid treatment on beef trimmings and subprimals against Salmonella and E. coli O157:H7 within regulatory retained water limitations - (Peer Reviewed Journal)
Kalchayanand, N., Arthur, T.M., Wang, R., Brown, T., Wheeler, T.L. 2024. Evaluation of peracetic acid treatment on beef trimmings and subprimals against Salmonella and E. coli O157:H7 within regulatory retained water limitations. Journal of Food Protection. 87(3). Article 100217. https://doi.org/10.1016/j.jfp.2024.100217.

Salmonella-induced microbiome profile in response to sanitation by quaternary ammonium chloride - (Peer Reviewed Journal)
Chen, Q., Palanisamy, V., Wang, R., Bosilevac, J.M., Chitapilly Dass, S. 2024. Salmonella-induced microbiome profile in response to sanitation by quaternary ammonium chloride. Microbiology Spectrum. 12(2). Article 02346-23. https://doi.org/10.1128/spectrum.02346-23.

Multiplex high resolution melt curve real-time PCR assay for detection of extended-spectrum Beta-lactam-resistant Shiga toxin-producing E. coli - (Peer Reviewed Journal)

Shotgun metagenomics reveals a highly diverse and communal microbial network present in the drains of three beef-processing plants - (Peer Reviewed Journal)

Identification of actionable sentinels for Salmonella contamination of beef lymph nodes and determining the impact of environmental contamination levels - (Abstract Only)
Schmidt, J.W., Fashenpour, E., Soler-Diaz, R., Harhay, D.M., Arthur, T.M., Bosilevac, J.M., Wheeler, T.L., Kang, Q., Gragg, S.E., Sanchez-Plata, M. 2023. Identification of actionable sentinels for Salmonella contamination of beef lymph nodes and determining the impact of environmental contamination levels. [Abstract]. American Meat Science Association Conference Reciprocal Proceedings. No. 117.

The effects of the administration of a Saccharomyces cerevisiae direct-fed microbial on the prevalence of Salmonella in bovine mesenteric lymph nodes - (Abstract Only)
Habib, K., Schmidt, J.W., Nichols, C.A., Kang, Q., Bosilevac, J.M., Harhay, D.M., Gragg, S.E. 2022. The effects of the administration of a Saccharomyces cerevisiae direct-fed microbial on the prevalence of Salmonella in bovine mesenteric lymph nodes. [Abstract]. International Association for Food Protection Proceedings. P1-83.

Fate of Shiga toxin-producing Escherichia coli (STEC) and Salmonella during kosher processing of fresh beef - (Peer Reviewed Journal)
Kalchayanand, N., Koohmaraie, M., Wheeler, T.L. 2023. Fate of Shiga toxin-producing Escherichia coli (STEC) and Salmonella during kosher processing of fresh beef. Journal of Food Protection. 86(6). Article 100088. https://doi.org/10.1016/j.jfp.2023.100088.

Characterizing the collective dynamics of microbial interactions in environmental multi-species biofilm using shotgun metagenomics - (Abstract Only)

Identification of dominant factors contributing to persistence of third-generation cephalosporin-resistant Salmonella at a beef cattle feedyard - (Abstract Only)
Schmidt, J.W., Murray, S.A., Dickey, A.M., Wheeler, T.L., Harhay, D.M., Arthur, T.M. 2023. Identification of dominant factors contributing to persistence of third-generation cephalosporin-resistant Salmonella at a beef cattle feedyard. [Abstract]. International Association for Food Protection Proceedings. T4-10.

Post abattoir risk-based meat safety assurance - (Abstract Only)
Bosilevac, J.M., Arthur, T.M., Wang, R. 2023. Post abattoir risk-based meat safety assurance [abstract]. Risk-based meat inspection and integrated meat safety assurance (RIBMINS) 3rd conference, March 29-30, 2023, Bucharest, Romania. P. 9.

Shiga toxin-producing Escherichia coli in pork, are they really only a problem for beef? - (Abstract Only)
Bosilevac, J.M., Guragain, M., Wang, R. 2023. Shiga toxin-producing Escherichia coli in pork, are they really only a problem for beef? [Abstract]. Safe Pork Conference, May 15-17, 2023, New Orleans, LA. Absract No. 16349.

Survival of Salmonella enterica and E. coli O157:H7 in environmental biofilms isolated from pork plants as compared to beef plants - (Abstract Only)
Wang, R., Bosilevac, J.M. 2023. Survival of Salmonella enterica and E. coli O157:H7 in environmental biofilms isolated from pork plants as compared to beef plants. [Abstract. Safepork. 14(1).

Efficacy of short thermal treatment time against Escherichia coli O157:H7 and Salmonella on the surface of fresh beef - (Peer Reviewed Journal)
Kalchayanand, N., Wang, R., Brown, T., Wheeler, T.L. 2023. Efficacy of short thermal treatment time against Escherichia coli O157:H7 and Salmonella on the surface of fresh beef. Journal of Food Protection. 86(3). Article 100040. https://doi.org/10.1016/j.jfp.2023.100040.

Determination of verification parameters for using the manual sampling device for fresh raw beef trim - (Peer Reviewed Journal)
Arthur, T.M., Brown, T., Wheeler, T.L. 2023. Determination of verification parameters for using the manual sampling device for fresh raw beef trim. Journal of Food Protection. 86. Article 100041. https://doi.org/10.1016/j.jfp.2023.100041.

Distribution of extremely heat-resistant Escherichia coli in the beef production and processing continuum - (Peer Reviewed Journal)
Guragain, M., Schmidt, J.W., Dickey, A.M., Bosilevac, J.M. 2023. Distribution of extremely heat-resistant Escherichia coli in the beef production and processing continuum. Journal of Food Protection. 86(1). Article 100031. https://doi.org/10.1016/j.jfp.2022.100031.

Universal hydrolysis probe-based approach for specific detection and genotyping of foodborne pathogens - (Peer Reviewed Journal)
Valez, F., Bosilevac, J.M., Mishra, A., Singh, P. 2023. Universal hydrolysis probe-based approach for specific detection and genotyping of foodborne pathogens. Journal of Microbiological Methods. 204. Article 106632. https://doi.org/10.1016/j.mimet.2022.106632.

Characterization of Salmonella strains and environmental microorganisms isolated from a meat plant with Salmonella recurrence - (Peer Reviewed Journal)
Wang, R., Dass, S.C., Chen, Q., Guragain, M., Bosilevac, J.M. 2022. Characterization of Salmonella strains and environmental microorganisms isolated from a meat plant with Salmonella recurrence. Meat and Muscle Biology. 6(1). Article 15442. https://doi.org/10.22175/mmb.15442.

A review of Shiga-toxin producing Escherichia coli (STEC) contamination in the raw pork production chain - (Peer Reviewed Journal)
Haque, M., Bosilevac, J.M., Chaves, B.D. 2022. A review of Shiga-toxin producing Escherichia coli (STEC) contamination in the raw pork production chain. International Journal of Food Microbiology. 377. Article 109832. http://doi.org/10.1016/j.ijfoodmicro.2022.109832.

Impact of “Deep Cleaning” sanitization on natural biofilm communities and the survival of Salmonella enterica - (Abstract Only)
Wang, R., Bosilevac, J.M. 2022. Impact of “Deep Cleaning” sanitization on natural biofilm communities and the survival of Salmonella enterica. [Abstract]. American Society of Microbiology (ASM) Conference on Biofilms 2022. November 13-17, 2022, Charlotte, NC, Control NO. 2022-A-75-ASM-BIO.

Efficacy of antimicrobial interventions used in meat processing plants against antimicrobial tolerant non-antibiotic-resistant and antibiotic-resistant Salmonella on fresh beef - (Peer Reviewed Journal)
Kalchayanand, N., Dass, S.C., Zhang, Y., Oliver, E.L., Wang, B., Wheeler, T.L. 2022. Efficacy of antimicrobial interventions used in meat processing plants against antimicrobial tolerant non-antibiotic-resistant and antibiotic-resistant Salmonella on fresh beef. Journal of Food Protection. 85(8):1114-1121. https://doi.org/10.4315/JFP-21-364.

Development and validation of high-resolution melting assays for the detection of potentially virulent strains of Escherichia coli O103 and O121 - (Peer Reviewed Journal)
Velez, F.J., Bosilevac, J.M., Delannoy, S., Fach, P., Nagpal, R., Singh, P. 2022. Development and validation of high-resolution melting assays for the detection of potentially virulent strains of Escherichia coli O103 and O121. Food Control. 139. Article 109095. https://doi.org/10.1016/j.foodcont.2022.109095.

Characterization and comparison of Salmonella spp. isolated from the mesenteric lymph nodes of cattle and the feedlot environment - (Abstract Only)

Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems - (Peer Reviewed Journal)
Guragain, M., Schmidt, J.W., Kalchayanand, N., Dickey, A.M., Bosilevac, J.M. 2022. Characterization of Escherichia coli harboring colibactin genes (clb) isolated from beef production and processing systems. Nature Scientific Reports. 12. Article 5305. https://doi.org/10.1038/s41598-022-09274-x.

Pathogenomes and variations in Shiga toxin production among geographically distinct clones of Escherichia coli O113:H21 - (Peer Reviewed Journal)
Allué-Guardia, A., Koenig, S.S., Martinez, R.A., Rodriguez, A.L., Bosilevac, J.M., Feng, P., Eppinger, M. 2022. Pathogenomes and variations in Shiga toxin production among geographically distinct clones of Escherichia coli O113:H21. Microbial Genomics. 8. Article 000796. https://doi.org/10.1099/mgen.0.000796.

Evaluation of Salmonella biofilm cell transfer from common food contact surfaces to beef products - (Peer Reviewed Journal)
Wang, R., King, D.A., Kalchayanand, N. 2022. Evaluation of Salmonella biofilm cell transfer from common food contact surfaces to beef products. Journal of Food Protection. 85(4):632-638. https://doi.org/10.4315/JFP-21-334.