Research Project: Strategies to Control Respiratory Diseases of Cattle

Location: Animal Health Genomics

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Introgression and disruption of migration routes have shaped the genetic integrity of wildebeest populations - (Peer Reviewed Journal)
Liu, X., Lin, L., Sinding, M.S., Bertola, L.D., Hanghoj, K., Quinn, L., Garcia-Erill, G., Rasmussen, M.S., Schubert, M., Pecnerova, P., Balboa, R.F., Li, Z., Heaton, M.P., Smith, T.P.L., Pinto, R., Wang, X., Kuja, J., Bruniche-Olsen, A., Meisner, J., Santander, C.G., Ogutu, J.O., Masembe, C., da Fonseca, R.R., Muwanika, V., Siegismund, H.R., Albrechtsen, A., Moltke, I., Heller, R. 2024. Introgression and disruption of migration routes have shaped the genetic integrity of wildebeest populations. Nature Communications. 15. Article 2921. https://doi.org/10.1038/s41467-024-47015-y.

Diversity and antigenic potentials of Mycoplasmopsis bovis secreted and outer membrane proteins within a core genome of strains isolated from North American bison and cattle - (Peer Reviewed Journal)
Wynn, E.L., Browne, A.S., Clawson, M.L. 2024. Diversity and antigenic potentials of Mycoplasmopsis bovis secreted and outer membrane proteins within a core genome of strains isolated from North American bison and cattle. Genome. pp. 1-6. https://doi.org/10.1139/gen-2023-0084.

African bush pigs exhibit porous species boundaries and appeared in Madagascar concurrently with human arrival - (Peer Reviewed Journal)
Balboa, R.F., Bertola, L., Bruniche-Olsen, A., Rasmussen, M., Liu, X., Besnard, G., Salmona, J., Santander, C.G., He, S., Zinner, D., Heaton, M.P., Smith, T.P., Moltke, I., Albrechtsen, A., Heller, R. et al. 2024. African bush pigs exhibit porous species boundaries and appeared in Madagascar concurrently with human arrival. Nature Communications. 15. Article 172. https://doi.org/10.1038/s41467-023-44105-1.

Early detection of infectious bovine keratoconjunctivitis with artificial intelligence - (Peer Reviewed Journal)
Gupta, S., Kuehn, L.A., Clawson, M.L. 2023. Early detection of infectious bovine keratoconjunctivitis with artificial intelligence. Veterinary Research. https://doi.org/10.1186/s13567-023-01255-w.

Two bovine hepacivirus genome sequences from U.S. cattle - (Peer Reviewed Journal)
Workman, A.M., Harhay, G.P., Groves, J.T., Vander Ley, B.L. 2024. Two bovine hepacivirus genome sequences from U.S. cattle. Journal of Veterinary Diagnostic Investigation. 36(2):274-277. https://doi.org/10.1177/10406387231225656.

The effect of probiotics in a milk replacer on leukocyte differential counts, phenotype, and function in neonatal dairy calves - (Peer Reviewed Journal)
Eicher, S.D., Kritshevsky, J.E., Bryan, K.A., Chitko-Mckown, C.G. 2023. The effect of probiotics in a milk replacer on leukocyte differential counts, phenotype, and function in neonatal dairy calves. Microorganisms. 11(11). Article 2620. https://doi.org/10.3390/microorganisms11112620.

Effect of probiotics in milk replacer on the microbiome of the lung in neonatal dairy calves - (Abstract Only)

African bush pigs exhibit porous species boundaries and appeared in Madagascar concurrently with human arrival - (Pre-print Publication)
Balboa, R.F., Bertola, L., Bruniche-Olsen, A., Rasmussen, M., Liu, X., Santander, C.G., He, S., Zinner, D., Heaton, M.P., Smith, T.P.L., Muwanika, V., Masembe, C., Schubert, M., Kuja, J., Quinn, L., Garcia-Erill, G., Lin, L., Wang, X., Moltke, I., Albrechtsen, A., Heller, R., et. al. 2023. African bush pigs exhibit porous species boundaries and appeared in Madagascar concurrently with human arrival. bioRxiv. https://doi.org/10.1101/2023.08.23.553838.

Probiotics in milk replacer affect the lung microbiome of neonatal dairy calves - (Abstract Only)

Detection of Mannheimia haemolytica-specific IgG, IgM and IgA in sera and their relationship to respiratory disease in cattle - (Peer Reviewed Journal)
Poonsuk, K., Kordik, C., Hille, M., Cheng, T., Crosby, W.B., Woolums, A., Clawson, M.L., Chitko-McKown, C., Brodersen, B., Loy, J.D. 2023. Detection of Mannheimia haemolytica-specific IgG, IgM and IgA in sera and their relationship to respiratory disease in cattle. Animals. 13(9). Article 1531. https://doi.org/10.3390/ani13091531.

From genomics to MALDI-TOF MS: Diagnostic identification and typing of bacteria in veterinary clinical laboratories - (Book / Chapter)
Loy, D., Clawson, M.L. 2023. From genomics to MALDI-TOF MS: Diagnostic identification and typing of bacteria in veterinary clinical laboratories. In: Shah, H.N., Gharbia, S.E., Shah, A.J., Tranfield, E.Y., Thompson, K.C., editors. Microbiological Identification using MALDI-TOF and Tandem Mass Spectrometry - Industrial and Environmental Applications. April 2023 edition. New York, NY: Wiley Press. p. 283-302.

First gene-edited calf with reduced susceptibility to a major viral pathogen - (Peer Reviewed Journal)
Workman, A.M., Heaton, M.P., Vander Ley, B., Webster, D., Sherry, L., Bostrom, J.R., Larson, S., Kalbfleisch, T., Harhay, G.P., Jobman, E., Carlson, D., Sonstegard, T.S. 2023. First gene-edited calf with reduced susceptibility to a major viral pathogen. Proceedings of the National Academy of Sciences-Nexus. 2(5). Article pgad125. https://doi.org/10.1093/pnasnexus/pgad125.

Does the upper respiratory tract microbiome change in dairy calves fed milk replacer containing probiotics? - (Abstract Only)

A CD46-edited calf with reduced susceptibility to bovine viral diarrhea virus - (Abstract Only)

Colonialism in Southern Africa leaves a lasting legacy of reduced genetic diversity in Cape buffalo - (Peer Reviewed Journal)
Quinn, L., Garcia-Erill, G., Santander, C., Bruniche-Olsen, A., Liu, X., Sinding, M.S., Heaton, M.P., Smith, T.P.L., Pecnerova, P., Bertola, L.D., Hanghoj, K., Rasmussen, M.S., De Jager, D., Siegismund, H.R., Albrechtsen, A., Heller, R., Moltke, I. 2023. Colonialism in Southern Africa leaves a lasting legacy of reduced genetic diversity in Cape buffalo. Molecular Ecology. Article 16851. https://doi.org/10.1111/mec.16851.

A single amino acid substitution in porcine reproductive and respiratory syndrome virus glycoprotein 2 significantly impairs its infectivity in macrophages - (Peer Reviewed Journal)
Chaudhari, J., Leme, R.A., Durazo-Martinez, K., Sillman, S., Workman, A.M., Vu, H.L.X. 2022. A single amino acid substitution in porcine reproductive and respiratory syndrome virus glycoprotein 2 significantly impairs its infectivity in macrophages. Viruses. 14(12). Article 2822. https://doi.org/10.3390/v14122822.

First gene-edited calf with reduced susceptibility to a major viral pathogen - (Pre-print Publication)
Workman, A.M., Heaton, M.P., Vander Ley, B.L., Webster, D.A., Sherry, L., Larson, S., Kalbfleisch, T.S., Harhay, G.P., Jobman, E.E., Carlson, D.F., Sonstegard, T.S. 2022. First gene-edited calf with reduced susceptibility to a major viral pathogen. bioRxiv. Article 519336. https://doi.org/10.1101/2022.12.08.519336.

Protein targets for preventative measures and diagnostics of Mycoplasma bovis from North American bison and cattle - (Abstract Only)

Genotype classification of Moraxella bovis using MALDI-TOF MS profiles - (Peer Reviewed Journal)
Olson, H.G., Loy, J.D., Clawson, M.L., Wynn, E.L., Hille, M.M. 2022. Genotype classification of Moraxella bovis using MALDI-TOF MS profiles. Frontiers in Microbiology. 13. Article 1057621. https://doi.org/10.3389/fmicb.2022.1057621.

Stress triggers expression of bovine herpesvirus 1 infected cell protein 4 (bICP4) RNA during early stages of reactivation from latency in pharyngeal tonsil - (Peer Reviewed Journal)
Toomer, G., Workman, A.M., Harrison, K.S., Stayton, E., Hoyt, P.R., Jones, C. 2022. Stress triggers expression of bovine herpesvirus 1 infected cell protein 4 (bICP4) RNA during early stages of reactivation from latency in pharyngeal tonsil. Journal of Virology. 96(23). Article e0101022. https://doi.org/10.1128/jvi.01010-22.

Whole genome sequencing of Moraxella bovis strains from North America reveals two genotypes with different genetic determinants - (Peer Reviewed Journal)
Wynn, E.L., Hille, M.M., Loy, J.D., Schuller, G., Kuhn, K.L., Dickey, A.M., Bono, J.L., Clawson, M.L. 2022. Whole genome sequencing of Moraxella bovis strains from North America reveals two genotypes with different genetic determinants. BMC Microbiology. 22(1). Article 258. https://doi.org/10.1186/s12866-022-02670-3.

Current and emerging diagnostic approaches to bacterial diseases of ruminants - (Review Article)
Loy, J.D., Clawson, M.L., Adkins, P.R.F., Middleton, J.R. 2023. Current and emerging diagnostic approaches to bacterial diseases of ruminants. Veterinary Clinics of North America, Food Animal Practice. 39:93-114. https://doi.org/10.1016/j.cvfa.2022.10.006.

First description of LPS-treated, pen-housed feeder cattle as a model of bacterial infection - (Abstract Only)

Recent emergence of bovine coronavirus variants with mutations in the hemagglutinin-esterase receptor binding domain in U.S. cattle - (Peer Reviewed Journal)
Workman, A.M., McDaneld, T.G., Harhay, G.P., Das, S., Loy, J.D., Hause, B.M. 2022. Recent emergence of bovine coronavirus variants with mutations in the hemagglutinin-esterase receptor binding domain in U.S. cattle. Viruses. 14(10). Article 2125. https://doi.org/10.3390/v14102125.

Changes in serum haptoglobin concentration in cattle administered LPS - (Abstract Only)
Touze, A., Chitko-Mckown, C.G. 2022. Changes in serum haptoglobin concentration in cattle administered LPS. In proceedings: 2022 National Veterinary Scholars Symposium, University of Minnesota, Minneapolis, Minnesota. August 4-7, 2022.Abstract. pg. 298.

A five year randomized controlled trial to assess the efficacy and antibody responses of a commercial and autogenous vaccine for the prevention of infectious bovine keratoconjunctivitis - (Peer Reviewed Journal)
Hille, M.M., Spangler, M.L., Clawson, M.L., Heath, K.D., Vu, H.L.X., Rogers, R.E.S., Loy, J.D. 2022. A five year randomized controlled trial to assess the efficacy and antibody responses of a commercial and autogenous vaccine for the prevention of infectious bovine keratoconjunctivitis. Vaccines. 10(6). Article 916. https://doi.org/10.3390/vaccines10060916.

Table 1. Genome sequence context for JMJD1C SNPs associated with bovine congestive heart failure (BCHF) - (Database / Dataset)
Heaton, M., Harhay, G., Smith, T.P.L., Kuhn, K., Kalbfleisch, T.S., Jobman, E., Vander Ley, B. 2022. Table 1. Genome sequence context for JMJD1C SNPs associated with bovine congestive heart failure (BCHF). Figshare. Dataset. https://doi.org/10.6084/m9.figshare.19780558.v1.

Ruminant-specific retrotransposons shape regulatory evolution of bovine immunity - (Peer Reviewed Journal)
Kelly, C.J., Chitko-McKown, C.G., Chuong, E.B. 2022. Ruminant-specific retrotransposons shape regulatory evolution of bovine immunity. Genome Research. 32(8):1474-1486. https://doi.org/10.1101/gr.276241.121.

Association of ARRDC3 and NFIA variants with bovine congestive heart failure in feedlot cattle - (Peer Reviewed Journal)
Heaton, M.P., Harhay, G.P., Bassett, A.S., Clark, H.J., Carlson, J.M., Jobman, E.E., Sadd, H.R., Pelster, M.C., Workman, A.M., Kuehn, L.A., Kalbfleisch, T.S., Piscatelli, H., Carrie, M., Krafsur, G.M., Grotelueschen, D.M., Vander Ley, B.L. 2022. Association of ARRDC3 and NFIA variants with bovine congestive heart failure in feedlot cattle. F1000Research. 11. Article 385. https://doi.org/10.12688/f1000research.109488.1 .

ADAM17 is an essential factor for the infection of bovine cells with pestiviruses - (Peer Reviewed Journal)
Zaruba, M., Chen, H., Pietsch, O.F., Szakmary-Braendle, K., Auer, A., Motz, M., Seitz, K., Dusterhoft, S., Workman, A.M., Rumenapf, T., Riedel, C. 2022. ADAM17 is an essential factor for the infection of bovine cells with pestiviruses. Viruses. 14(2). Article 381. https://doi.org/10.3390/v14020381.

Ruminant-specific retrotransposons shape regulatory evolution of bovine immunity - (Pre-print Publication)
Kelly, C.J., Chitko-Mckown, C.G., Chuong, E.B. 2021. Ruminant-specific retrotransposons shape regulatory evolution of bovine immunity. bioRxiv. https://doi.org/10.1101/2021.10.01.462810.