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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Microbial and Chemical Food Safety » Research » Publications at this Location » Publication #311290
Title: Speciation of AsIII and AsV in fruit juices by dispersive liquid–liquid microextraction and hydride generation-atomic fluorescence spectrometry

Author
item LAI, GUOXIN - Meizhouwan Vocational Technology College
item Chen, Guoying

Submitted to: Journal of Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/13/2015
Publication Date: 5/15/2015
Publication URL: http://handle.nal.usda.gov/10113/60924
Citation: Lai, G., Chen, G. 2015. Speciation of AsIII and AsV in fruit juices by dispersive liquid–liquid microextraction and hydride generation-atomic fluorescence spectrometry. Journal of Food Chemistry. DOI: 10.1016/j.foodchem.2015.05.052.

Interpretive Summary: Presence of arsenic in fruit juices causes health concerns worldwide, especially inorganic arsenic that may pose long term health consequences. A study was conducted to develop a new method to quantitatively detect different types of inorganic arsenic using atomic fluorescence spectrometry (AFS). The result of this study may enhance the safety of fruit juice and prevent diseases caused by inorganic arsenic.

Technical Abstract: A new procedure was developed to speciate and quantify As(III) and As(V) in fruit juices. At pH 3.0, As(III) and ammonium pyrrolidine dithiocarbamate (APDC) formed a complex, which was extracted into carbon tetrachloride by dispersive liquid–liquid microextraction (DLLME) and subsequently quantified by hydride generation-atomic fluorescence spectrometry (HG-AFS). After As(V) was reduced by thiosulphate at pH 1.8, total inorganic arsenic (iAs) was determined following the same protocol. As(V) was calculated from the difference. Interference from methylarsonous acid, MMA(III), was reduced by controlling pH during reduction. This method is applicable to most fruit juices except pear juice that contains considerable MMA. This procedure achieved 92-102% recovery at 10 ug/L fortification, and 0.03-1.2 ug/L limit of detection (3).