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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #343539
Title: Adaption of roots to nitrogen deficiency revealed by 3D quantification and proteomic analysis

Author
item QIN, LU - Oil Crops Research Institute - China
item WALK, THOMAS - North Dakota State University
item HAN, PEIPEI - Oil Crops Research Institute - China
item LIYU, CHEN - Fujian Agriculture And Forest University
item SHENG, ZHANG - Cornell University
item LI, YINSHUI - Oil Crops Research Institute - China
item HU, XIAOJIA - Oil Crops Research Institute - China
item XIE, LIHUA - Oil Crops Research Institute - China
item Yang, Yong
item Liu, Jiping
item LU, XING - South China Agricultural Univerisity
item YU, CHANGBING - Oil Crops Research Institute - China
item TIAN, JIANG - South China Agricultural Univerisity
item SHAFF, JON - Cornell University
item KOCHIAN, LEON - University Of Saskatchewan
item LIAO, XING - Oil Crops Research Institute - China
item LIAO, HONG - Fujian Agriculture And Forest University

Submitted to: Plant Physiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/2/2018
Publication Date: 11/19/2018
Citation: Qin, L., Walk, T., Han, P., Liyu, C., Sheng, Z., Li, Y., Hu, X., Xie, L., Yang, Y., Liu, J., Lu, X., Yu, C., Tian, J., Shaff, J., Kochian, L.V., Liao, X., Liao, H. 2018. Adaption of roots to nitrogen deficiency revealed by 3D quantification and proteomic analysis. Plant Physiology. 179:329-347. https://doi.org/10.1104/pp.18.00716.
DOI: https://doi.org/10.1104/pp.18.00716

Interpretive Summary: Nitrogen (N) is the most required nutrient for plant. However, mechanisms underlying plant adaption to N deficiency remain largely unclear. Here, severe inhibition of rapeseed by N deficiency was first verified in the field. Root elongation, branching, and solidity quantified using 2D and 3D root analysis systems, were significantly altered by N deficiency. TMT-based proteomics was further employed to reveal mechanisms of rapeseed root adaption to N deficiency. Totally 10788 proteins were identified, with 175 and 756 differentially expressed proteins (DEPs) found in short- and long-term N deficient roots, respectively. The majority of DEPs were annotated to be involved in single-organism transport, cellular macromolecule metabolism and protein metabolism. In addition, the abundance of proteins involved in cell wall organization was highly enhanced in N deficient roots. Notably, most identified peroxidases were less abundant in N deficient roots, and peroxidase activities were also decreased. Besides, some DEPs were also associated with amino acid and carbohydrate metabolisms, as revealed by protein-protein interaction network. Taken together, these results comprehensively provide insights into morphological and physiological changes of rapeseed roots in response to N deficiency, and elucidate possible molecular mechanisms underlying these responses. In the future, this work might help to improve rapeseed N use efficiency through traditional breeding and genetic engineering techniques.

Technical Abstract: Proteomics approaches were used to study the dynamics of protein expression in response to N deficiency in rapeseed. Differentially expressed proteins (DEPs) were found and their annotated cellular roles identified. This work might help to improve rapeseed N use efficiency through traditional breeding and genetic engineering techniques.