Location: Mycotoxin Prevention and Applied Microbiology Research
Title: Ochratoxin A analysis in wine and grape juice using LC-fluorescence detection with nanosponge solid phase extraction clean-upAuthor
|
Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 4/28/2020 Publication Date: 8/29/2019 Citation: Appell, M., Evans, K.O., Jackson, M.A., Compton, D.L. 2019. Ochratoxin A analysis in wine and grape juice using LC-fluorescence detection with nanosponge solid phase extraction clean-up [abstract]. Interpretive Summary: Technical Abstract: One of the most common techniques to quantitate ochratoxin A contamination in fruit beverages is liquid chromatography analysis using fluorescence detection (LC-FLD). A new method was developed to improve the detection of ochratoxin A in wine and grape juice using a novel beta-cyclodextrin polyurethane polymer for solid phase extraction clean-up prior to LC-FLD. Thermolytic polymer synthesis with methylene bis-diphenyl diisocyanate produced a crosslinked polymer suitable for ochratoxin A binding. The polyurethane moieties were observed by FT-IR spectroscopy and experimental spectra correspond with PM6 semi-empirical calculated spectra. Atomic force microscopy analysis identified features of the ß-cyclodextrin polyurethane polymer on the nanoscale. The LC-FLD method coupled with nanosponge solid phase extraction clean-up achieved a limit of detection for ochratoxin A of 0.2 ng/mL and quantitative detection between 20 ng/mL to 0.5 ng/mL. |
