Reviving the Transcriptome Studies: An insight into the emergence of Single-Molecule Transcriptome Sequencing

Authors: WANG, BO - Cold Spring Harbor Laboratory; KUMAR, VIVEK - Cold Spring Harbor Laboratory; OLSON, ANDREW - Cold Spring Harbor Laboratory; Ware, Doreen

Submitted to: Frontiers in Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/9/2019
Publication Date: 4/26/2019
Citation: Wang, B., Kumar, V., Olson, A., Ware, D. 2019. Reviving the Transcriptome Studies: An insight into the emergence of Single-Molecule Transcriptome Sequencing. Frontiers in Genetics. 10:384. https://doi.org/10.3389/fgene.2019.00384.
DOI: https://doi.org/10.3389/fgene.2019.00384

Interpretive Summary: We provide an overview of single-molecule sequencing platform and its diverse applications in various biological studies, and our perspective on the challenges associated with the transcriptome studies.

Technical Abstract: Advances in RNA sequencing technology have provided an exceptional opportunity to study functional implications of the genetic variability. However, traditional transcriptome studies have been limited by the length of short-reads sequencing. With the development of single-molecule sequencing from PacBio, a growing number of studies on the transcriptome of different organisms have been reported. Single-molecule sequencing has emerged as advantageous for novel genes/isoforms identification, lncRNA and fusion transcripts identification, and a comprehensive genome annotation. This approach can be used across a broad spectrum of species to better interpret the coding information of the genome, and facilitate the biological function study. We provide an overview of single-molecule sequencing platform and its diverse applications in various biological studies, and our perspective on the challenges associated with the transcriptome studies.