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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #365651
Research Project: Database Tools for Managing and Analyzing Big Data Sets to Enhance Small Grains Breeding

Location: Plant, Soil and Nutrition Research

Title: Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance

Author
item FRITSCHE-NETO, ROBERTO - Universidade De Sao Paulo
item OLIVEIRA DE SOUZA, THIAGO - Embrapa
item PEREIRA, HELTON - Embrapa
item CLAUDIO DE FARIA, LUIS - Embrapa
item MELA, LEONARDO - Embrapa
item NOVAES, EVANDRO - Federal University Of Goias
item BRUM, ITARAJU - Cornell University
item Jannink, Jean-Luc

Submitted to: Scientia Agricola
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/23/2018
Publication Date: 7/1/2019
Citation: Fritsche-Neto, R., Oliveira De Souza, T.L., Pereira, H.S., Claudio De Faria, L., Mela, L.C., Novaes, E., Brum, I.J., Jannink, J. 2019. Association mapping in common bean revealed regions associated with Anthracnose and Angular Leaf Spot resistance. Scientia Agricola. 76(4):321-327. http://doi.org/10.1590/1678-992X-2017-0306.
DOI: https://doi.org/10.1590/1678-992X-2017-0306

Interpretive Summary: Common bean anthracnose (ANT) and angular leaf spot (ALS) can cause yield losses of up to 80% and occur in more than 60 countries around the world. We evaluated 60 elite inbred lines under field conditions in Brazil for severity of ALS and ANT to identify genes capable of increasing resistance to these diseases. We found genes that on their own can reduce disease severity by about 25% for ANT and 20% for ALS. These markers will be useful to increase ANT and ALS resistance in new carioca and black seeded bean cultivars in Brazil.

Technical Abstract: Despite important biotic stresses to common bean, Anthracnose (ANT) and Angular Leaf Spot (ALS) can cause losses of up to 80% and occur in more than 60 countries around the world. Genetic resistance is the most sustainable strategy to manage these diseases. Thus, we aimed to (1) identify new SNP markers associated with ALS and ANT resistance loci in elite common bean lines, and (2) provide a functional characterization of the DNA sequences containing the identified SNP markers. We evaluated 60 inbred lines, under field conditions, which represent the elite germplasm developed by the Embrapa common bean breeding program across 22 years, in terms of severity of the ALS and ANT. The lines were genotyped with 5,398 SNPs. Then, a Mixed Linear Model was run to determine the SNP-trait associations. We observed two-significant marker-trait associations reacting to ANT, both located on chromosome Pv-02. These markers explained 25 % of the phenotypic variation. For ALS, only one significant marker-trait association was observed, which is located in chromosome Pv-10 and explained 19% of the phenotypic variation. These markers, along with others already used, will be useful to add or keep ANT and ALS resistance loci identified in this work in the new carioca and black seeded cultivars.