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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #366130
Research Project: Bioavailability of Iron, Zinc and Select Phytochemicals for Improved Health (Bridging Project)

Location: Plant, Soil and Nutrition Research

Title: Alterations in gut microflora populations and brush border functionality following intra-amniotic administration (Gallus gallus) of wheat bran prebiotics extracts

Author
item WANG, XUAN - CHINA AGRICULTURAL UNIVERSITY
item KOLBA, NIKOLAI
item LIANG, JIANFEN - CHINA AGRICULTURAL UNIVERSITY
item TAKO, ELAD

Submitted to: Food & Function
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/1/2019
Publication Date: 7/5/2019
Citation: Wang, X., Kolba, N.J., Liang, J., Tako, E.N. 2019. Alterations in gut microflora populations and brush border functionality following intra-amniotic administration (Gallus gallus) of wheat bran prebiotics extracts. Food & Function. 1-10. https://doi.org/10.1039/C9FO00836E.
DOI: https://doi.org/10.1039/C9FO00836E

Interpretive Summary: Wheat bran is the by-product of milling wheat flour which is one of the richest sources of dietary fiber. Wheat bran soluble extracts may improve intestinal health and function. By using the chicken embryo as a model, we tested and introduced wheat bran extracts during incubation, followed by samples collection at hatch. The results indicated that wheat bran extracts improved intestinal functionality and development, and increased the abundance of health promoting bacteria.

Technical Abstract: Wheat bran is the by-product of milling wheat flour which is one of the richest sources of dietary fiber, and cellulase that can be used for increasing the soluable dietary fiber. We hypothesize that wheat bran prebiotics would improve mineral-metabolism gene expression, intestinal functionality, and increase health-promoting bacterial populations in vivo (Gallus gallus). By using the intra-amniotic administration procedure, five groups (non-injected; 18O H2O; 100 mg/mL wheat bran prebiotics; 100 mg/mL cellulase wheat bran prebiotics; 50 mg/mL Arbinose) were utilized. Upon hatch, cecum, small intestine and liver were collected for assessment. The results indicated that wheat bran prebiotics significantly up regulated the expression of certain brush border membrane functional genes, and mineral metabolism related proteins, in addition to increase the intestinal beneficial bacterial populations. Prebiotics from wheat bran, cellulase wheat bran and arabinose increased the intestinal villi height, and goblet cell diameters and numbers.