Characterization of two types of polysaccharides from Eremurus hissaricus roots growing in Tajikistan

Authors: MUHIDINOV, Z - Chemistry Institute Of Tajikistan Academy Of Science; BOBOKALONOV, J - Chemistry Institute Of Tajikistan Academy Of Science; ISMOILOV, I - Chemistry Institute Of Tajikistan Academy Of Science; Strahan, Gary; Chau, Hoa - Rose; Hotchkiss, Arland; Liu, Linshu

Submitted to: Food Hydrocolloids
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/12/2020
Publication Date: 2/13/2020
Citation: Muhidinov, Z.K., Bobokalonov, J.T., Ismoilov, I.B., Strahan, G.D., Chau, H.K., Hotchkiss, A.T., Liu, L.S. 2020. Characterization of two types of polysaccharides from Eremurus hissaricus roots growing in Tajikistan. Food Hydrocolloids. 205. https://doi.org/10.1016/j.foodhyd.2020.105768.
DOI: https://doi.org/10.1016/j.foodhyd.2020.105768

Interpretive Summary: The wildflower known as Eremurus hissaricus, foxtail lily or desert candle, is a native plant from Eastern Europe to Central Asia. E. hissaricus roots are rich in bioactive carbohydrates and are considered to have huge economic potential for development. However, the composition and fine structure of those bioactive carbohydrates haven’t been well characterized. For the first time, we isolated and purified the two major types of carbohydrates from E. hissaricus roots, measured their size, and identified the backbone structures. The research provided basic scientific information, paving the way for subsequent research on their applications.

Technical Abstract: Eremurus Hissaricus (E.hissaricus) is a native plant of Tajikistan that has been used in traditional medicine and whose raw components have been identified as having the potential for the domestic economy. We have found that carbohydrates comprise 80-85% of the root biomass, from which we have characterized two types of polysaccharides: A water-soluble polysaccharide (WSP) and an acid-soluble polysaccharide (ASP), with 23% and 8% yields, respectively. The results from FTIR, HPAEC-PAD, HPSEC and NMR spectroscopy (1H, 13C, zTOCSY, HSQC, HSQCTOXY and HMBC) demonstrated that WSP has a backbone of (1-4)-linked ß-D-mannopyranosyl and ß-D-glucopyranosyl residues in a 1.5:1 molar ratio, having acetylated groups, with weight-average molecular weights of 495 kDa. Analyses of ASP indicate that it is comprised of D-galactose, D-mannose, and D-glucose in a molar ratio of 1.8: 1.6: 1.0, which is consistent with an acetylated galactoglucomannan polysaccharide with (1-4)-linked ß-pyranose configurations, and weight-average molecular weights of 179 kDa. The FTIR absorption bands in the region of 880-890 cm-1 confirms the presence of ß-pyranose configuration of main polysaccharide backbone in the both ASP and WSP samples. These polysaccharides are an available raw material with the potential for use in a number of industrial applications.