Combination of Ultrasound and Chlorogenic acid for Inactivation of planktonic and biofilm cells of Pseudomonas fluorescens

Authors: SUN, JINYUE - Jiangsu Academy Agricultural Sciences; Huang, Lihan; SUN, ZHILAN - Jiangsu Academy Agricultural Sciences; WANG, DEBAO - Jiangsu Academy Agricultural Sciences; LIU, FANG - Jiangsu Academy Agricultural Sciences; DU, LIHUI - Nanjing University Of Finance And Economics; WANG, DAOYING - Jiangsu Academy Agricultural Sciences

Submitted to: Food Control
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/10/2022
Publication Date: 3/1/2022
Citation: Sun, J., Huang, L., Sun, Z., Wang, D., Liu, F., Du, L., Wang, D. 2022. Combination of Ultrasound and Chlorogenic acid for Inactivation of planktonic and biofilm cells of Pseudomonas fluorescens. Food Control. 155. https://doi.org/10.1016/j.foodres.2022.111009.
DOI: https://doi.org/10.1016/j.foodres.2022.111009

Interpretive Summary: Foodborne pathogens and spoilage microorganisms can form biofilms on food contact surfaces that are difficult to remove, causing potential outbreaks of foodborne illness. This study investigates the combined application of ultrasound (US) and chlorogenic acid (CA) for inactivating biofilms using Pseudomonas fluorescens as a test microorganism. The results show that the combined application of US and CA is very effective in inactivating the biofilm cells. The method may be used in the food industry to improve the hygienic conditions on food contact surfaces, thus potentially improving the safety of the food supply.

Technical Abstract: This study investigated the synergistic efficacy of ultrasound (US, 50 kHz, 400W) in combination with chlorogenic acid (CA; 0, 0.5, 1, and 2%) to inactivate Pseudomonas fluorescens planktonic and biofilm cells. The P. fluorescens planktonic and biofilm cells were treated with CA with and without US for 5, 10, 20, 30, or 60 min. Results showed that US enhanced the efficacy of CA for inactivation of both P. fluorescens planktonic and biofilm cells. Treatment with 2% CA and US could completely inactivate P. fluorescens planktonic cells within 10 min and the biofilm cells within 30 min. Confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM), ATP, and nucleic acid release assays indicated that the combination of US and CA could quickly damage the integrity of P. fluorescens planktonic and biofilm cell membranes. The combined treatment effectively inactivates P. fluorescens planktonic and biofilm cells via the synergetic destruction of the biofilm structure and bacterial cell integrity.