Efficacy of a mixed peroxyorganic acid antimicrobial wash solution against Salmonella, Escherichia coli O157:H7 or Listeria monocytogenes on cherry tomatoes

Authors: Gurtler, Joshua; DONG, XIAOLING - Mantrose-Haeuser Co, Inc; ZHONG, BIN - Mantrose-Haeuser Co, Inc; LEE, RENSUN - Mantrose-Haeuser Co, Inc

Submitted to: Journal of Food Protection
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/25/2022
Publication Date: 1/27/2022
Citation: Gurtler, J., Dong, X., Zhong, B., Lee, R. 2022. Efficacy of a mixed peroxyorganic acid antimicrobial wash solution against Salmonella, Escherichia coli O157:H7 or Listeria monocytogenes on cherry tomatoes. Journal of Food Protection. 85(5):773–777. https://doi.org/10.4315/JFP-21-368.
DOI: https://doi.org/10.4315/JFP-21-368

Interpretive Summary: A study was conducted to evaluate a new organic produce wash composed of a new combination of organic acids and hydrogen peroxide. The wash, composed of lactic acid, hydrogen peroxide and one or more fruit acids, was challenged against Salmonella enterica, E. coli O157:H7, or Listeria monocytogenes in suspension or on the surface of dip-inoculated cherry tomatoes. The new solution killed more than 99.99999% of Salmonella, and E. coli O157:H7 in solution and killed approximately 99.999% of L. monocytogenes in solution. The new solution killed more than 99.9% of all three pathogens on tomatoes.

Technical Abstract: A study was conducted to evaluate a new organic produce wash composed of a combination of organic acids and hydrogen peroxide, in the form of a mixed peroxyacid solution, against foodborne pathogens. The mixed peroxyacid, composed of lactic acid, hydrogen peroxide and one or more fruit acids, was challenged against Salmonella enterica, E. coli O157:H7, or Listeria monocytogenes in suspension or on the surface of dip-inoculated cherry tomatoes. Cherry tomatoes were also treated with 8 ppm of free chlorine added in the form of sodium hypochlorite (NaOCL). When tested against planktonic cells in pure culture for 120 s, Salmonella, and E. coli O157:H7 were reduced by 7.5 and 7.1 log CFU/ml with 0.40% of the peroxyacid solution, respectively, while L. monocytogenes decreased by 5.0 log by treatment with a 0.80% solution. When cherry tomatoes were dip-inoculated and treated with 8 ppm free chlorine, Salmonella and E. coli O157:H7 populations decreased by 2.5 and 2.6 log, respectively, which was not significantly different than reductions incurred by sterile water rinses. A 1.0% solution of peroxyacid solution, however, reduced the same populations by 3.8 and 3.4 log CFU/tomato, respectively, which was significantly greater (P < 0.05) than reductions achieved by the 2 min sterile water rinse. When dip-inoculated with L. monocytogenes, and treated, populations were reduced by 3.5 log CFU/tomato with 1.0% peroxyacid solution which was greater (P < 0.05) than reductions achieved by 8 ppm Cl (2.6 log) or sterile water (1.7 log). Results demonstrate that this peroxyacid combination antimicrobial wash may be an effective organic produce wash for preventing cross-contamination during the washing of cherry tomatoes, as well as being capable of inactivating up to 3.8, 3.4, and 3.5 log CFU/tomato of S. enterica, E. coli O157:H7 and L. monocytogenes, respectively.