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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Quality and Safety Assessment Research Unit » Research » Publications at this Location » Publication #404434
Research Project: Assessment of Quality Attributes of Poultry Products, Grain, Seed, Nuts, and Feed

Location: Quality and Safety Assessment Research Unit

Title: Proteomics on postmortem meat with white striping myopathy

Author
item Kong, Byungwhi
item OWENS, CASEY - University Of Arkansas
item BOTTJE, WALTER - University Of Arkansas
item SHAKERI, MAJID - Oak Ridge Institute For Science And Education (ORISE)
item CHOI, JANGHAN - Oak Ridge Institute For Science And Education (ORISE)
item Zhuang, Hong
item Bowker, Brian

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/26/2023
Publication Date: 9/7/2023
Citation: Kong, B.C., Owens, C., Bottje, W., Shakeri, M., Choi, J., Zhuang, H., Bowker, B.C. 2023. Proteomics on postmortem meat with white striping myopathy. Meeting Abstract. No. OP-43: 67.

Interpretive Summary:

Technical Abstract: White striping (WS) is a major meat quality defect that results in significant economic losses on the global poultry industry. WS is the occurrence of white lines of raw breast fillets. The exact etiologies for WS are still unclear. Proteomic analyses of co-expressed WS and woody breast phenotypes previously demonstrated dysfunctions on carbohydrates metabolism, protein synthesis and calcium buffering capabilities in muscle cells. In this study, we conducted shotgun proteomics with WS only meat samples collected at post-mortem stage. Chicken breast fillets were collected at approximately 6 h postmortem from a university pilot plant. After determining WS severity, protein extractions were conducted from WS meat with no WB condition (n=5) and normal non-affected (no WS) control meats (n=5). Shotgun proteomics was conducted by Orbitrap Lumos, tandem mass tag (TMT) analysis. Proteins were identified using a Scaffold program, and differential abundance were calculated using t-tests. The annotations of proteins and pathway analyses were performed using Ingenuity Pathway Analysis software. In this study, 146 differentially abundant proteins (|fold change|>1.4; p value < 0.05) were identified in the WS meats compared with controls. The significant canonical pathways include serine biosynthesis, aldosterone signaling, protein ubiquitination, and NAD salvage pathway. The potential upstream regulators include LIPE, let-7, NID1, UCP1 and KLF15. The results of this study provide additional insights into the cellular mechanisms on WS myopathy and meat quality.