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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Quality and Safety Assessment Research Unit » Research » Publications at this Location » Publication #404448
Research Project: Assessment of Quality Attributes of Poultry Products, Grain, Seed, Nuts, and Feed

Location: Quality and Safety Assessment Research Unit

Title: Proteomics of enriched mitochondria from postmortem meat with woody breast myopathy

Author
item Kong, Byungwhi
item BOTTJE, WALTER - University Of Arkansas
item OWENS, CASEY - University Of Arkansas
item SHAKERI, MAJID - Oak Ridge Institute For Science And Education (ORISE)
item CHOI, JANGHAN - Oak Ridge Institute For Science And Education (ORISE)
item Zhuang, Hong
item Bowker, Brian

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/10/2023
Publication Date: 7/10/2023
Citation: Kong, B.C., Bottje, W., Owens, C., Shakeri, M., Choi, J., Zhuang, H., Bowker, B.C. 2023. Proteomics of enriched mitochondria from postmortem meat with woody breast myopathy. Poultry Science Association Meeting Abstract. 102/198.

Interpretive Summary:

Technical Abstract: Woody breast (WB) myopathy is a major meat quality defect distinguished by increased hardness of raw breast fillets. The exact etiologies for WB are still unclear. Mitochondrial dysfunctions have been documented previously. In this study, we conducted shotgun proteomics with mitochondrial fraction enriched from postmortem meat samples. Chicken breast fillets were collected at approximately 6 h postmortem from a university pilot plant. After determining WB severity, protein extractions from WB (n=5) and non-affected control samples (n=5) were conducted on enriched mitochondrial fraction samples. Shotgun proteomics was conducted by Orbitrap Lumos, tandem mass tag (TMT) analysis. Proteins were identified using a Scaffold program, and differential abundances were calculated by student t-tests. The annotations of proteins and pathway analyses were performed using Ingenuity Pathway Analysis software. In this study, 194 differentially abundant proteins (|fold change|>1.5; p value < 0.05) were identified in the mitochondrial fractions of WB meat. The significant canonical pathways include EIF2 signaling, glycolysis I, calcium signaling, and gluconeogenesis. The potential upstream regulators include AK1, NR4A1, CNBP, CPT1B, and AR and core molecules for causal networks include CPT1B, SMTNL1, PPARGC1A, COL6A1, and LIPE. The results of this study provide specific insights into a role that mitochondria may play in the WB myopathy.