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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #409538
Research Project: Mapping Crop Genome Functions for Biology-Enabled Germplasm Improvement

Location: Plant, Soil and Nutrition Research

Title: Characterization of key regulators in microRNA-mediated responses to phosphate deficiency in Arabidopsis root involved in root development

Author
item ZHANG, LIFANG - Cold Spring Harbor Laboratory
item OLSON, ANDREW - Cold Spring Harbor Laboratory
item LEE YOUNG, KOUNG - Cold Spring Harbor Laboratory
item FAHEY, AUDREY - Cold Spring Harbor Laboratory
item HU, FANGLE - Cold Spring Harbor Laboratory
item REGULSKI, MICHAEL - Cold Spring Harbor Laboratory
item GAUDINIER, ALLISON - University Of California, Davis
item BAGMAN, ANNE-MAARIT - University Of California, Davis
item BRADY, SIOBHAN - University Of California, Davis
item Ware, Doreen

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/22/2023
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Plant microRNAs play crucial roles in regulating plant development and stress responses by post-transcriptionally repressing the expression of their target genes. Phosphorus (P) is an essential nutrient for plant development, and its deficiency impairs plant growth, consequently reducing crop yield. Phosphate (Pi) is the major form of P. Under Pi-starvation, plants have evolved various adaptive responses to P stress, including alterations in root system architecture (RSA) and the transcription profiles of many vital genes involved in Pi movement throughout the plant. MicroRNAs have been identified as essential components in this adaptive process. To identify key transcription factors acting as cis-regulators of these miRNAs, we constructed a gene regulatory network (GRN) by mapping protein-DNA interactions (PDI) through yeast one-hybrid (Y1H) screening of the promoter regions of Pi-responsive microRNAs and their targets. By integrating this network with public transcriptome profiles under phosphate-limiting conditions, we were able to predict important TF candidates. Subsequently, we obtained T-DNA insertion lines of 15 TF candidates and assessed their root phenotype under phosphate starvation conditions. We successfully identified three TFs that can antagonize primary root growth inhibition under Pi-limiting conditions.