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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Foodborne Toxin Detection and Prevention Research » Research » Publications at this Location » Publication #409857
Research Project: Novel Methods for the Mitigation of Human Pathogens and Mycotoxin Contamination of High Value California Specialty Crops

Location: Foodborne Toxin Detection and Prevention Research

Title: Complete genome sequence of Citrobacter braakii ASE1 generated by PacBio sequencing

Author
item Tran, Thao
item Lee, Sangin
item Hnasko, Robert
item McGarvey, Jeffery - Jeff

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/5/2024
Publication Date: 2/14/2024
Citation: Tran, T.D., Lee, S., Hnasko, R.M., McGarvey, J.A. 2024. Complete genome sequence of Citrobacter braakii ASE1 generated by PacBio sequencing. Microbiology Resource Announcements. 13(3). Article e01000-23. https://doi.org/10.1128/MRA.01000-23.
DOI: https://doi.org/10.1128/MRA.01000-23

Interpretive Summary: We report the DNA sequence of the genome of the bacterium Citrobacter braakii ASE1 that was isolated form the soil of a lettuce farm in Salinas, California in 2020. This bacterium has been shown to inhibit the growth of the human pathogenic bacteria Escherichia coli and Salmonella enterica. Sequence analysis revealed that the genome is one single circular chromosome of 5,021,820 base pairs with a GC content of 52.2%.

Technical Abstract: We report the complete genome sequence of Citrobacter braakii ASE1 generated by the PacBio sequel II platform. This bacterium was isolated from the soil of a lettuce farm in Salinas, California in 2020 and was shown to inhibit the growth of Salmonella enterica serovar Poona and Escherichia coli serotype O157:H7 an in vitro fluorescence based assay. The genome consists of a single circular chromosome of 5,021,820 bp with a 52.2% GC content.