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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Quality and Safety Assessment Research Unit » Research » Publications at this Location » Publication #410782
Research Project: Assessment of Quality Attributes of Poultry Products, Grain, Seed, Nuts, and Feed

Location: Quality and Safety Assessment Research Unit

Title: Multi-omics analyses of chicken breast with spaghetti meat myopathy

Author
item SHAKERI, MAJID - Oak Ridge Institute For Science And Education (ORISE)
item Kong, Byungwhi
item CHOI, JANGHAN - Oak Ridge Institute For Science And Education (ORISE)
item Zhuang, Hong
item Bowker, Brian

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/15/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Spaghetti meat (SM) is an emerging chicken breast myopathy characterized by a loss of tissue integrity in the breast muscle resulting in the meat surface having loosely connected muscle fibers resembling spaghetti pasta. To understand biochemical and physiological alterations occurring in SM, we conducted multi-omics analyses including proteomics, metabolomics, and lipidomics using a liquid chromatography-mass spectrometry (LC-MS) method. Differentially abundant proteins, metabolites, and lipid species were identified in SM compared with normal control (CON) meat and the results were integrated for biological interpretations. Chicken breast fillets (8 CON, 8 SM) were collected from commercial broiler chickens at a commercial processing plant. Breast muscle samples were dissected into smaller pieces and flash frozen in liquid nitrogen. Chicken breast meat samples were homogenized by bead blasting, homogenates were digested by trypsin, and lysates were extracted. Meat extracts were subjected to LC-MS analysis. Differential abundances were calculated using t-tests and calculated p-values were adjusted to false discovery rate (FDR). A total of 2593 molecules, including 1903 proteins, 506 lipids, 181 metabolites, and 3 electrolytes were identified. Of those, differentially abundant molecules, based on FDR < 0.05, included 151 proteins, 17 lipids, 17 metabolites, and no electrolytes. Differentially abundant proteins, lipids, and metabolites indicated that SM exhibited: 1) increased nonsense mediated decay pathways; 2) decreased glucose metabolism pathways; 3) decreased contents of NAD+ and lactic acid; 4) increased triglycerides (TG); and 5) decreased plasmenylphosphatidylcholines (Plasmenyl-PC), acylcarnitines (AC), and phosphatidic acids (PA). Results in this study provide insights into biochemical alterations that may be associated with susceptibilities of chicken breast to SM condition and quality of SM defects in chicken breast meat.