Skip to main content
ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #417378
Research Project: Genetic Regulation of Fruit and Vegetable Nutritional Quality and Maturation and Technology Development

Location: Plant, Soil and Nutrition Research

Title: Real-time library search (RTLS) triggered MS3 facilitates in-depth cannabinoid profile in different hemp genotypes and identification of novel cannabinoids

Author
item LUI, ANDY - Cornell University
item ANDERSON, ELIZABETH - Cornell University
item Ramsey, John - John
item Hernandez, Katherine
item Yang, Yong
item Stansell, Zachary
item Gordon, Terry
item Thannhauser, Theodore - Ted
item ZHANG, SHENG - Cornell University

Submitted to: American Society for Mass Spectrometry
Publication Type: Abstract Only
Publication Acceptance Date: 3/4/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cannabis sativa, including hemp and marijuana, has recently emerged as a prominent crop for both industrial production and academic research. Current legal guidelines rely on total THC content ('9-THC + THCA) to distinguish between hemp (< 0.3% total dry weight) and marijuana. Nonetheless, cannabis biosynthesizes a wide array of cannabinoids, many of which have not been structurally or functionally characterized. Elucidation of the complete cannabinoid profile in different hemp and marijuana genotypes will facilitate selective breeding, pharmacological research, and decision making by regulatory bodies. Here, we employed LC-MSn-based untargeted metabolomics with several MS3 acquisition methodologies with real-time library search for in-depth cannabinoid profiling of three hemp genotypes. These results will provide insight into cannabinoid biosynthesis and facilitate novel cannabinoid discovery.