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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #417527
Research Project: Championing Improvement of Sorghum and Other Agriculturally Important Species through Data Stewardship and Functional Dissection of Complex Traits

Location: Plant, Soil and Nutrition Research

Title: Characterization of key regulators in microRNA-mediated responses to phosphate deficiency in Arabidopsis root development

Author
item ZHANG, LIFANG - Cold Spring Harbor Laboratory
item OLSON, ANDREW - Cold Spring Harbor Laboratory
item FAHEY, AUDREY - Cold Spring Harbor Laboratory
item HU, FANGLE - Cold Spring Harbor Laboratory
item REGULSKI, MICHAEL - Cold Spring Harbor Laboratory
item GAUDINIER, ALLISON - University Of California, Davis
item BAGMAN, ANNE-MAARIT - University Of California, Davis
item BRADY, SIOBHAN - University Of California, Davis
item Ware, Doreen

Submitted to: Arabidopsis Research International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/15/2024
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Plant microRNAs (miRNA) play crucial roles in regulating plant development and stress responses by post-transcriptionally repressing the expression of their target genes. Phosphorus (P) is an essential nutrient for plant development and its deficiency impairs plant growth, consequently, reducing crop yield. Phosphate (Pi) is the major form of P. Under Pi-starvation, plants have evolved various adaptive responses to P stress, including alterations in root system architecture (RSA) and the transcription profiles of many vital genes, including miRNAs involved in Pi homeostasis throughout the plant. Several miRNAs have been identified as essential components in this adaptive process. To identify key transcription factors acting as cis regulators of these miRNAs, we constructed a gene regulatory network (GRN) by mapping protein-DNA interactions (PDIs) from yeast one-hybrid (Y1H) screenings of the promoter regions of Pi-responsive miRNAs and their targets. By integrating this network with public transcriptome profiles under Pi-limiting conditions, we were able to predict important TF candidates. Subsequently, we obtained T-DNA insertion lines of 15 TF candidates and assessed their root phenotype under Pi-starvation conditions. We successfully identified T-DNA lines of three different TF that result in greater primary root growth compared to wild-type under Pi-limiting conditions.