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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Plant, Soil and Nutrition Research » Research » Publications at this Location » Publication #419743
Research Project: Biochemistry and Physiology of Crop Adaptation to Soil-Based Abiotic Stresses

Location: Plant, Soil and Nutrition Research

Title: Tonoplast-localized OsTIP2;1 is involved in aluminum detoxification in rice

Author
item ZHANG, WEIYIN - Guangzhou University
item YANG, SHAOHUA - Fujian Academy
item FENG, YIMENG - Guangzhou University
item LIANG, BIXIA - Guangzhou University
item LI, CHUNE - Guangzhou University
item LI, QIAN - Guangzhou University
item ZHENG, ZHENSHUN - Guangzhou University
item JI, XIYUE - Guangzhou University
item WANG, YUQI - Guangzhou University
item Liu, Jiping

Submitted to: Plant Physiology and Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/20/2024
Publication Date: 8/28/2024
Citation: Zhang, W., Yang, S., Feng, Y., Liang, B., Li, C., Li, Q., Zheng, Z., Ji, X., Wang, Y., Liu, J. 2024. Tonoplast-localized OsTIP2;1 is involved in aluminum detoxification in rice. Plant Physiology and Biochemistry. Volume 215, 109063. https://doi.org/10.1016/j.plaphy.2024.109063.
DOI: https://doi.org/10.1016/j.plaphy.2024.109063

Interpretive Summary: Aluminum (Al) stress presents a serious challenge in acidic soils, greatly impacting crop growth and yield. While rice is particularly resilient to Al toxicity, the internal mechanisms of this tolerance are still not fully understood. Our research identified a transporter located on the tonoplast in rice that helps move excess aluminum from the cytosol to the vacuole in root cells, which is a crucial internal detoxification process. These findings uncover a new mechanism for aluminum resistance in rice and could serve as a guideline to enhance aluminum resistance in other crop species.

Technical Abstract: We examined the role of OsTIP2;1, a tonoplast-bound intrinsic protein (TIP), in rice’s internal Al detoxification. OsTIP2;1 expression was quickly and explicitly activated by Al ions in roots but not in shoots. The OsTIP2;1-GFP protein localizes to the tonoplast in plant and yeast cells. Non-functional ostip2;1 rice mutants were more vulnerable to Al toxicity. In the roots, the ostip2;1 mutants exhibited considerably lower levels of Al in the cell sap, primarily the vacuolar contents, than in the wild-type plant. Moreover, the ostip2;1 mutants showed reduced Al accumulation in the roots but increased translocation to the shoots. Heterologous expression of tonoplast-localized OsTIP2;1 in yeast led to enhanced Al tolerance, suggesting that OsTIP2;1 facilitates Al sequestration to the vacuole.