Charles Lee
Bioproducts Research
Acting Research Leader
Phone: (510) 559-5858
Fax: (510) 559-5818
(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
Publications
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will take you to the publication reprint.)
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Effect of starch and paperboard reinforcing structures on insulative fiber foam composites
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Glenn, G.M., Tonoli, G., Silva, L., Klamczynski, A.P., Wood, D.F., Chiou, B., Lee, C.C., Hart-Cooper, W.M., Mccaffrey, Z., Orts, W.J. 2024. Effect of starch and paperboard reinforcing structures on insulative fiber foam composites. Polymers. 16(7):911. https://doi.org/10.3390/polym16070911.
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Compostable bioplastics from dairy processing coproducts
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Upcycling whey for production of compostable bioplastics
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Polyhydroxyalkanoate production by Priestia megaterium on glycerol
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Polyhydroxyalkanoate production in Pseudomonas putida from alkanoic acids
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Cal, A.J., Sikkema, D., Hathwaik, U.I., Orts, W.J., Lee, C.C. 2023. Polyhydroxyalkanoate production in Pseudomonas putida from alkanoic acids. PLOS. 18(7). https://doi.org/10.1371/journal.pone.0284377.
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Compression molded cellulose fiber foams
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Glenn, G.M., Orts, W.J., Klamczynski, A.P., Shogren, R., Hart-Cooper, W.M., Wood, D.F., Lee, C.C., Chiou, B. 2023. Compression molded cellulose fiber foams. Cellulose. 30:3489-3503. https://doi.org/10.1007/s10570-023-05111-0.
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Wounding and cold stress increase resin and rubber production of Parthenium argentatum cultivar G711
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Placido, D., McMahan, C.M., Lee, C.C. 2022. Wounding and cold stress increase resin and rubber production of Parthenium argentatum cultivar G711. Industrial Crops and Products. 193. Article 116174. https://doi.org/10.1016/j.indcrop.2022.116174.
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Potential of industrial hemp for phytoremediation of heavy metals
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Placido, D., Lee, C.C. 2022. Potential of industrial hemp for phytoremediation of heavy metals. Plants. 11(5). Article 595. https://doi.org/10.3390/plants11050595.
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Isolation of endophytes that suppress pathogen growth of chickpea (Cicer arietinum L.)
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Nadeem, A., O'Keeffe, T.L., Cal, A.J., Palumbo, J.D., Arshad, R., Bibi, N., Lee, C.C. 2022. Isolation of endophytes that suppress pathogen growth of chickpea (Cicer arietinum L.). Pakistan Journal of Botany. 54(5):1813-1820. https://doi.org/10.30848/pjb2022-5(22).
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Production of poly(3-hydroxybutyrate-co-4-hydroxybutryate) copolymers in Bacillus megaterium
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Production of polyhydroxyalkanoate copolymers containing 4-hydroxybutyrate in engineered Bacillus megaterium
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Cal, A.J., Kibblewhite, R.E., Sikkema, D.W., Torres, L.F., Hart-Cooper, W.M., Orts, W.J., Lee, C.C. 2020. Production of polyhydroxyalkanoate copolymers containing 4-hydroxybutyrate in engineered Bacillus megaterium. International Journal of Biological Macromolecules. 168:86-92. https://doi.org/10.1016/j.ijbiomac.2020.12.015.
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Biodegradation of cotton, PET, and PHB fibers in compost conditions
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Lactic acid bacteria for growth promotion and fungal disease management in crops
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Nadeem, A., Bibi, N., Arshad, R., Lee, C.C. 2019. Lactic acid bacteria for growth promotion and fungal disease management in crops. Meeting Abstract. [abstract]. ASM Microbe 2019 conference, San Francisco, CA. June 20-24, 2019.
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Polyhydroxyalkanoate production in Pseudomonas putida from alkanoic acids
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Sikkema, D.W., Cal, A.J., Hathwaik, U.I., Kibblewhite, R.E., Orts, W.J., Lee, C.C. 2019. Polyhydroxyalkanoate production in Pseudomonas putida from alkanoic acids. Meeting Abstract. [abstract]. ASM Microbe 2019 conference, San Francisco, CA. June 20-24, 2019.
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Nucleation and plasticization with recycled low-molecular-weight poly-3-hydroxybutyrate toughens virgin poly-3-hydroxybutyrate
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Cal, A.J., Grubbs, B., Torres, L.F., Riiff, T.J., Orts, W.J., Lee, C.C. 2019. Nucleation and plasticization with recycled low-molecular-weight poly-3-hydroxybutyrate toughens virgin poly-3-hydroxybutyrate. Journal of Applied Polymer Science. 136(17):47432. https://doi.org/10.1002/app.47432.
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Polyhydroxyalkanoate production in pseudomonas
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Sikkema, D., Cal, A.J., Ponce, M.I., Orts, W.J., Lee, C.C., Hathwaik, U.I. 2018. Polyhydroxyalkanoate production in pseudomonas. Meeting Abstract. 25th Annual BEPS Conference. September 20-22, 2017. Albany, CA.
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Isolation of ligninolytic Bacillus sp. strains for depolymerization of alkali lignin
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Shah, T.A., Tabassum, R., Orts, W.J., Lee, C.C. 2019. Isolation of ligninolytic Bacillus sp. strains for depolymerization of alkali lignin. Journal of Environmental Progress and Sustainable Energy. 38(3):e13036. https://doi.org/10.1002/ep.13036.
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Biochemical characterization of caulobacter crescentus xylose dehydrogenase
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Lee, C.C., Jordan, D.B., Stoller, R.J., Kibblewhite, R.E., Wagschal, K.C. 2018. Biochemical characterization of caulobacter crescentus xylose dehydrogenase. International Journal of Biological Macromolecules. 118:1362-1367. https://doi.org/10.1016/j.ijbiomac.2018.06.124.
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Biochemical characterization of caulobacter crescentus xylose dehydrogenase
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Lee, C.C., Jordan, D.B., Stoller, J.R., Kibblewhite, R.E., Wagschal, K.C. 2018. Biochemical characterization of caulobacter crescentus xylose dehydrogenase. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2018.06.124.
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Absence or presence of metal ion activation in two structurally
similar GH43 beta-xylosidases
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Jordan, D.B., Stoller, J.R., Kibblewhite, R.E., Lee, C.C., Wagschal, K.C. 2018. Analysis of divalent-metal requirements of two GH43 ß-xylosidases by site-directed mutagenesis. Enzyme and Microbial Technology. 114:29-32. doi: 10.1016/j.enzmictec.2018.03.007.
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Production and uses of low molecular weight 3-hydroxybutyrate polymers and oligomers
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Cal, A.J., Grubbs, B., Torres, L.F., Riiff, T.J., Orts, W.J., Lee, C.C. 2017. Production and uses of low molecular weight 3-hydroxybutyrate polymers and oligomers. Meeting Abstract. 25th Annual BEPS Conference. September 20-22, 2017. Albany, CA.
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A general correction to initial rates determined for nonprocessive exo-depolymerases acting on both substrate and product
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Stoller, J.R., Wagschal, K.C., Lee, C.C., Jordan, D.B. 2017. A general correction to initial rates determined for nonprocessive exo-depolymerases acting on both substrate and product. Analytical Biochemistry. 523:46-49. doi: 10.1016/j.ab.2017.02.004.
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Conversion of biomass to value-added bioproducts by enzyme nanoassemblies
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Lee, C.C., Kibblewhite, R.E., Paavola, C.D., Wagschal, K.C. 2017. Conversion of biomass to value-added bioproducts by enzyme nanoassemblies. Meeting Abstract. American Chemical Society. p.100.
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Production and characterization of microbial polyhydroxyalkanoates
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Cal, A.J., Sikkema, D.W., Ponce, M.I., Orts, W.J., Lee, C.C. 2017. Production and characterization of microbial polyhydroxyalkanoates. Meeting Abstract. American Chemical Society. p.340.
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Biochemical characterization of a GH43 ß-xylosidase from Bacteroides ovatus
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Jordan, D.B., Stoller, J.R., Lee, C.C., Chan, V.J., Wagschal, K.C. 2016. Biochemical characterization of a GH43 ß-xylosidase from Bacteroides ovatus. Applied Biochemistry and Biotechnology. 182:250-260. doi: 10.1007/s12010-016-2324-0.
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Production of D-xylonic acid from hemicellulose using artificial enzyme complexes
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Lee, C.C., Kibblewhite, R.E., Paavola, C., Orts, W.J., Wagschal, K.C. 2017. Production of D-xylonic acid from hemicellulose using artificial enzyme complexes. Journal of Microbiology and Biotechnology. 27(1):77–83 doi: 10.4014/jmb.1606.06041.
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Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth
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Sharon, J.A., Hathwaik, L.T., Glenn, G.M., Imam, S.H., Lee, C.C. 2016. Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth. Journal of Soil Science and Plant Nutrition. 16(2):525-536.
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Nanostructured enzyme assemblies for lignocellulosic biomass breakdown for bioproduct and bioenergy applications
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Lee, C.C., Wagschal, K.C., Paavola, C. 2016. Nanostructured enzyme assemblies for lignocellulosic biomass breakdown for bioproduct and bioenergy applications.[abstract]. USDA/NIFA Nanotechnology Meeting.
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Expression and characterization of hyperthermostable exo-polygalacturonase TtGH28 from Thermotoga thermophilus
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Wagschal, K.C., Stoller, J.R., Chan, V.J., Lee, C.C., Grigorescu, A.A., Jordan, D.B. 2016. Expression and characterization of hyperthermostable exo-polygalacturonase TtGH28 from Thermotoga thermophilus. Molecular Biotechnology. 58(7):509-519. doi: 10.1007/s12033-016-9948-8.
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Production of glucaric acid from hemicellulose substrate by rosettasome enzyme assemblies
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Lee, C.C., Kibblewhite, R.E., Paavola, C., Orts, W.J., Wagschal, K.C. 2016. Production of glucaric acid from hemicellulose substrate by rosettasome enzyme assemblies. Molecular Biotechnology. 58:489.
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Methanotrophic production of copolymer, poly(hydroxybutyrate-co-hydroxyvalerate), with high hydroxyvalerate content
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Cal, A.J., Ponce, M.I., Franquivillanueva, D.M., Orts, W.J., Pieja, A., Lee, C.C. 2016. Methanotrophic production of copolymer, poly(hydroxybutyrate-co-hydroxyvalerate), with high hydroxyvalerate content. International Journal of Biological Macromolecules. 87:302.
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Isolation and divalent-metal activation of a ß-xylosidase, RUM630-BX
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Jordan, D.B., Braker, J.D., Wagschal, K.C., Stoller, J.R., Lee, C.C. 2015. Isolation and divalent-metal activation of a ß-xylosidase, RUM630-BX. Enzyme and Microbial Technology. 82:158-163. doi: 10.1016/j.enzmictec.2015.10.001.
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Utilization of microbes to improve crop production
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Sharon, J.A., Lee, C.C., Glenn, G.M. 2015. Utilization of microbes to improve crop production. UJNR meeting, Kanazawa, Ishikawa, Japan. November 14 - 19, 2015. Meeting Proceedings. 44:29-30.
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X-ray crystal structure of divalent metal-activated ß-xyloisdase, RS223BX
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Jordan, D.B., Braker, J.D., Wagschal, K., Lee, C.C., Chan, V.J., Dubrovska, I., Anderson, S., Wawrzak, Z. 2015. X-ray crystal structure of divalent metal-activated ß-xyloisdase, RS223BX. Applied Biochemistry and Biotechnology. 177:637-648. doi: 10.1007/s12010-015-1767-z.
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Enzyme nanoassemblies for biomass conversion
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Lee, C.C., Wagschal, K.C., Paavola, C. 2015. Enzyme nanoassemblies for biomass conversion. Meeting Abstract. Gordon Research Conference Abstracts, 1(10).
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Nanostructure enzyme assemblies for biomass conversion
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Lee, C.C., Kibblewhite, R.E., Templeton, P.D., Orts, W.J., Paavola, C., Wagschal, K.C. 2015. Nanostructure enzyme assemblies for biomass conversion. 37th Symposium for Biotechnology. Meeting Abstract. PSI: M110.
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Biochemical characterization of uronate dehydrogenases from three Pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans
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Wagschal, K.C., Jordan, D.B., Lee, C.C., Younger, A.R., Braker, J.D., Chan, V.J. 2014. Biochemical characterization of uronate dehydrogenases from three Pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans. Enzyme and Microbial Technology. 69:62-68.
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Lignocellulose hydrolysis by multienzyme complexes
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Templeton, P.D., Kibblewhite, R.E., Wagschal, K.C., Paavola, C., Lee, C.C. 2014. Lignocellulose hydrolysis by multienzyme complexes. American Chemical Society Abstracts. Biol (68).
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Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation
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Singh, S.K., Heng, C., Braker, J.D., Chan, V.J., Lee, C.C., Jordan, D.B., Yuan, L., Wagschal, K.C. 2013. Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation. Journal of Industrial Microbiology and Biotechnology. 41(3):489-498. doi: 10.1007/s10295-013-1377-0.
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Expression and characterization of Coprothermobacter proteolyticus alkaline serine protease
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Majeed, T., Tabassum, R., Orts, W.J., Lee, C.C. 2013. Expression and characterization of Coprothermobacter proteolyticus alkaline serine protease. The Scientific World. doi: 10.1155/2013/396156.
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Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity
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Jordan, D.B., Lee, C.C., Wagschal, K., Braker, J.D. 2013. Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity. Archives of Biochemistry and Biophysics. 533:79-87.
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Divalent metal activation of a GH43 ß-xylosidase
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Lee, C.C., Braker, J.D., Grigorescu, A.A., Wagschal, K.C., Jordan, D.B. 2013. Divalent metal activation of a GH43 ß-xylosidase. Enzyme and Microbial Technology. 52(2):84-90.
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Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC-ICP-MS and HPLC-ESI-MS/MS
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Gong, L., Xu, Q., Lee, C.C., Zhang, H. 2012. Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC-ICP-MS and HPLC-ESI-MS/MS. European Food Research and Technology. 235(1):169-176.
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Isolation and characterization of a novel GH67 a-glucuronidase from a mixed culture
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Lee, C.C., Wagschal, K.C., Ruiping, L., Robertson, G.H., Kibblewhite, R.E., Orts, W.J. 2012. Isolation and characterization of a novel GH67 a-glucuronidase from a mixed culture. Journal of Industrial Microbiology and Biotechnology. 39:1245-1251. DOI:10.1007/s10295-012-1128-7.
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Development of enzymes and enzyme systems by genetic engineering to convert biomass to sugars
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Lee, C.C., Wagschal, K.C. 2013. Development of enzymes and enzyme systems by genetic engineering to convert biomass to sugars. [Abstract}. California American Chemical Society Section Meeting, April 25, 2012, Albany CA.
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Nanostructured enzyme assemblies for lignocellulosic biomass breakdown for bioproduct and bioenergy applications
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Microplate-based active/inactive 1 screen for biomass degrading enzyme library purification and gene discovery
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Wagschal, K.C., Lee, C.C. 2012. Microplate-based active/inactive 1 screen for biomass degrading enzyme library purification and gene discovery. Analytical Biochemistry. 89: 83-85.
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Plant cell walls to ethanol.
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Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.
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Isolation of alpha-glucuronidase enzyme from a rumen metagenomic library
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Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Li, R., Orts, W.J. 2012. Isolation of alpha-glucuronidase enzyme from a rumen metagenomic library. Protein Journal. 31(3):206-211.
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HILIC separation mechanisms of tetracyclines on amino bonded silica column
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Li, R., Zhang, Y., Lee, C.C., Liu, L., Huang, Y. 2011. HILIC separation mechanisms of tetracyclines on amino bonded silica column. Journal of Separation Science. 34(13):1508-1516.
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A high-throughput protein expression system in Pichia pastoris using a newly developed episomal vector
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Sasagawa, T., Moriya, S., Lee, C.C., Kitamoto, K., Arioka, M. 2011. A high-throughput protein expression system in Pichia pastoris using a newly developed episomal vector. Plasmid Journal. 65(1):65-69.
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Screening and characterization of hemicellulose-degrading enzymes
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Lee, C.C., Wagschal, K.C., Orts, W.J. Screening and characterization of hemicellulose-degrading enzymes. 2010 SIM Annual Meeting and Exhibition; Abstract S160b: p101.
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Screening assays for biomass-degrading enzymes
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Lee, C.C. 2010. Screening assays for biomass-degrading enzymes. Biofuels. 1(4):575-588.
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Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures
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Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures. Meeting Abstract. 85.
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Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water
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Li, R., Zhang, Y., Lee, C.C., Lu, R., Huang, Y. Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water. Journal of Chromatography, 2010. A 1217(11), pp 1799-1805.
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Cloning and characterization of alpha-glucuronidase enzyme
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Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.
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Cloning and characterization of alpha-glucuronidase enzyme
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Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.
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Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion
- (Peer Reviewed Journal)
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Wong, D., Batt Throne, S.B., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2010. Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion. Industrial Biotechnology. 6:112-118.
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Cloning and characterization of an alpha-glucuronidase from a mixed microbial population
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Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Robertson, G.H., Orts, W.J. 2009. Cloning and characterization of an alpha-glucuronidase from a mixed microbial population. Enzyme and Microbial Technology. 155(1-3):314-320.
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Molecular cloning and characterization of multidomain xylanase from manure library
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Li, R., Kibblewhite, R.E., Orts, W.J., Lee, C.C. 2009. Molecular cloning and characterization of multidomain xylanase from manure library. World Journal of Microbiology and Biotechnology. doi:10:1007/s11274-009-011106
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Engineer Sccharomyces cerevisiae for consolidated bioprocessing
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Wong, D., Batt Throne, S.B., Wan, D., Lee, C.C., Wagschal, K.C. 2009. Engineer Sccharomyces cerevisiae for consolidated bioprocessing. Meeting Abstract.
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Cloning biomass-degrading enzymes from environmental sources
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Lee, C.C., Wong, D., Wagschal, K.C., Orts, W.J. 2009. Cloning biomass-degrading enzymes from environmental sources. Meeting Abstract.
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Multimeric hemicellulases facilitate biomass conversion
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Zhanmin, F., Wagschal, K.C., Wei, C., Montross, M., Lee, C.C., Yuan, L. 2009. Multimeric hemicellulases facilitate biomass conversion. Proceedings of the National Academy of Sciences. 75(6):1754-1757.
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An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening
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Lee, C.C., Wagschal, K.C., Kibblewhite, R.E., Orts, W.J., Robertson, G.H., Wong, D. 2009. An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening. Applied Biochemistry and Biotechnology. 155(1-3):314-320.
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Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08
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Wagschal, K.C., Heng, C., Lee, C.C., Robertson, G.H., Orts, W.J., Wong, D. 2008. Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08. Applied Biochemistry and Biotechnology. 155(1-3):1-10.
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The Construction and Characterization of Two Xylan-degrading Chimeric Enzymes
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Fan, Z., Wagschal, K.C., Lee, C.C., Kong, Q., Shen, K.A., Maiti, I.B., Yuan, L. 2009. The Construction and Characterization of Two Xylan-degrading Chimeric Enzymes. Biotechnology and Bioengineering. 102:684-692
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Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture
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Wagschal, K.C., Heng, C., Lee, C.C., Wong, D. 2009. Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture. Applied Microbiology and Biotechnology. 81:855-863.
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Metagenomic gene discovery and enzyme engineering for biomass conversion
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Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. Metagenomic gene discovery and enzyme engineering for biomass conversion. American Chemical Society Western Regional Meeting. American Chemical Society Symposium: New Developments in Biofuels Research. Abstract 204.
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Gene discovery and engineering for biomass degradation
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Lee, C.C., Wagschal, K.C., Wong, D. 2008. Gene discovery and engineering for biomass degradation. United States-Japan Cooperative Program in Natural Resources. 2008. 50-51.
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Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme
- (Peer Reviewed Journal)
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Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. 2008. Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme. Current Microbiology. 57:301-305.
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Biofuels Group NSF DUSEL Project
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Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.
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An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening
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Lee, C.C., Wagschal, K.C., Accinelli, R., Orts, W.J., Robertson, G.H., Wong, D. 2008. An alpha-glucuronidase enzyme activity assay adaptable for solid phase screening. Biotechnology for Fuels and Chemicals Symposium Proceedings. Abstract 6-26.
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Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125
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Wagschal, K.C., Franquivillanueva, D.M., Lee, C.C., Robertson, G.H., Wong, D. Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125. Applied Biochemistry and Biotechnology.
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Cloning and characterization of xylanase gene from hot spring microorganism
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Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. Cloning and characterization of xylanase gene from hot spring microorganism. Meeting Abstract #307.
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Developing enzyme systems for biomass deconstruction
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Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2007. Developing enzyme systems for biomass deconstruction. Meeting Abstract. Paper No. 217
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SYNERGISTIC ACTION OF ALPHA-AMYLASE AND GLUCOAMYLASE ON THE HYDROLYSIS OF NATIVE STARCH GRANULES
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Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C., Wagschal, K.C. 2007. Synergistic action of alpha-amylase and glucoamylase on the hydrolysis of native starch granules. Protein and Peptide Letters. 26:159-164.
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SATB1 Packages Densely Looped, Transcriptionally Active Chromatin for Coordinated Expression of Cytokine Genes
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Cai, S., Lee, C.C., Kohwi-Shigematsu, T. 2006. Satb1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes. Nature Genetics. (38):1278-1288
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EXPRESSION AND SECRETION OF CLOSTRIDIUM CELLULOVORANS XYNA IN SACCHAROMYCES CEREVISIAE
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Batt, S.B., Wong, D., Wagschal, K.C., Lee, C.C. 2006. Expression and secretion of clostridium cellulovorans xyna in saccharomyces cerevisiae. Division of Biological Chemistry, Enzymes Program. Abstract 220.
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GENETIC AND BIOCHEMICAL CHARACTERIZATION OF A NOVEL BACTERIAL A-L-ARABINOFURANOSIDASE
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Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Accinelli, R., Robertson, G.H., Wong, D. 2007. Genetic and biochemical characterization of a novel bacterial a-l-arabinofuranosidase. Enzyme and Microbial Technology. (40):747-753.
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EVOLUTIONARY ENZYME DESIGN FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES
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Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2006. Evolutionary enzyme design for improved biorefining of crops and residues. Technical Program (abstract). P8.
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SYNERGISTIC ACTION OF BARLEY ALPHA-AMYLASE AND LENTINULA EDODES GLUCOAMYLASE ON RAW STARCH HYDROLYSIS
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Wong, D., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2006. Synergistic action of barley alpha-amylase and lentinula edodes glucoamylase on raw starch hydrolysis. Approved 10/27/2005. American Society of Biochemistry & Molecular Biology, San Francisco, CA. April 1-4, 2006, Program 73.16, vol A27.
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CLONING AND CHARACTERIZATION OF COLD-ACTIVE XYLANASE ENZYMES
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Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41.
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CLONING AND CHARACTERIZATION OF A COLD ACTIVE XYLANASE ENZYME
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Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of a cold active xylanase enzyme. Extremophiles. 10:295-300.
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NATIVE OR RAW STARCH DIGESTION
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Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Orts, W.J. 2006. Native or raw starch digestion: A Key step in energy efficient biorefining of grain. Journal of Agricultural and Food Chemistry, 54:353-65.
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ISOLATION AND CHARACTERIZATION OF A PSYCHROPHILIC XYLANASE ENZYME FROM FLAVOBACTERIUM SP.
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Lee, C.C., Smith, M.R., Accinelli, R., Williams, T.G., Wagschal, K.C., Wong, D., Robertson, G.H. 2006. Isolation and characterization of a psychrophilic xylanase enzyme from flavobacterium sp. Current Microbiology, 52(2):112-116.
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CHARACTERIZATION OF ACTIVE LENTINULA EDODES GLUCOAMYLASE EXPRESSED AND SECRETED BY SACCHAROMYCES CEREVISIAE
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Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Characterization of active lentinula edodes glucoamylase expressed and secreted by saccharomyces cerevisiae. Protein Journal. vol 24, pp 455-463.
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CHARACTERIZATION AND DIRECTED EVOLUTION OF HEMICELLULOTIC ENZYMES USING NATURAL XYLOOLIGOACCHARIDE SUBSTRATES
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Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D.W.S. 2005. Characterization of hemicellulotic degrading enzymes using natural substrates. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. 1B-65. Abstract A10, Enzyme Engineering XVIII.
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ENZYME-COUPLED ASSAY FOR BETA-XYLOSIDASE HYDROLYSIS OF NATURAL SUBSTRATES
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Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D. 2005. Enzyme-coupled assay for beta-xylosidase hydrolysis of natural substrates. Applied and Environmental Microbiology. 71:5318-5323.
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EVOLVED ENZYMES FOR COLD OR NON-COOKING HYDROLYSIS OF STARCHES TO FERMENTATION FEEDSTOCK
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Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R. 2005. Evolved enzymes for cold or non-cooking hydrolysis of starches to fermentation feedstock. In Proceedings of the North American Grain Congress, February 20-22, 2005, Reno, NV. 2005 CDROM.
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EXPRESSION AND CHARACTERIZATION OF ACTIVE LENTINUS EDODES GLUCOAMYLASE IN SACCHAROMYCES CEREVISIAE
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Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Expression and characterization of active. lentinus edodes glucoamylase in saccharomyces cerevisiae. Presentation 04:05. Paper No 11277.
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CLONING AND CHARACTERIZATION OF BIOMASS DEGRADING ENZYMES
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Lee, C.C., Accinelli, R., Batt, S.B., Williams, T.G., Wong, D., Wagschal, K.C., Robertson, G.H. 2005. Cloning and characterization of biomass degrading enzymes. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. IB-41 (Abstract).
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DIRECTED EVOLUTION OF BARLEY ALPHA-AMYLASE FOR INCREASED ACTIVITY
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Batt, S.B., Wong, D., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2004. Directed evolution of barley alpha-amylase for increased activity. American Chemical Society Western Regional Meeting, Sacramento, CA. Paper No. 144 (Abstracts).
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IN VITRO EVOLUTION OF HIGH-ACTIVITY BARLEY ALPHA-AMYLASE FOR RAW STARCH DIGESTION
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Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C. 2004. In vitro evolution of high-activity barley alpha-amylase for raw starch digestion. American Association of Cereal Chemists Meetings, September 19-22, 2004, San Diego, California. Abstract.
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HETEROGENEOUS BIOCATALYIC LIQUEFACTION AND SACCHARIFICAATION OF STARCH FOR ETHANOL
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Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C. 2004. Heterogeneous biocatalyic liquefaction and saccharificaation of starch for ethanol. Corn Utilization Conference Proceedings, June 6-9, 2004, Indianapolis, Indiana, Abstract.
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HIGH-ACTIVITY BARLEY ALPHA-AMYLASE BY DIRECTED EVOLUTION
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Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. High-activity barley alpha-amylase by directed evolution. Journal of Protein Chemistry, 23:53-460.
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RAPID SELECTION OF ALPHA-AMYLASE MUTANTS IN YEAST LIBRARIES FOR RAW STARCH ACTIVITY
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Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Rapid selection of alpha-amylase mutants in yeast libraries for raw starch activity. 2003. Society of Biomolecular Screening Annual Conference, Portland, Oregon.
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ENGINEERING APHA-AMYLASE FOR ENHANCED ACTIVITY BY MOLECULAR EVOLUTION
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Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Engineering apha-amylase for enhanced activity by molecular evolution. American Society of Biochemistry and Molecular Biology Annual Meeting, June 12-16, 2004, Boston, Massachusetts. Abstract No. B38.
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AN EPISOMAL EXPRESSION VECTOR FOR SCREENING MUTANT GENE LIBRARIES IN PICHIA PASTORIS
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Lee, C.C., Williams, T.G., Wong, D., Robertson, G.H. 2004. An episomal expression vector for screening mutant gene libraries in pichia pastoris. Plasmid Journal. 54(1):80-85.
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DIRECT SCREENING OF LIBRARIES OF YEAST CLONES FOR ALPHA-AMYLASE ACTIVITY ON RAW STARCH
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Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch. Protein and Peptide Letters. 10:459-468.
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CLONING AND CHARACTERIZATION OF THE XYN11A GENE FROM LENTINULA EDODES
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Lee, C.C., Wong, D., Robertson, G.H. 2005. Cloning and characterization of the xyn11a gene from lentinula edodes. Protein Journal. 24(1):21-26.
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ARS News Articles
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