Research Project: Curation and Research to Safeguard and Expand Collections of Plant and Microbial Genetic Resources and Associated Descriptive Information

Location: Agricultural Genetic Resources Preservation Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

An overview of pollen cryopreservation - (Book / Chapter)
Volk, G.M., Shepherd, A.N., Chen, K.Y. 2025. An overview of pollen cryopreservation. In: Volk, G.M., Chen, K., editors. Pollen Preservation. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/pollenpreservation/chapter/an-overview-of-pollen-cryopreservation/

Prunus pollen cryopreservation - (Book / Chapter)
DeBuse, C., Shepherd, A.N., Volk, G.M. 2025. Prunus pollen cryopreservation. In: Volk, G.M., Chen, K., editors. Pollen Preservation. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/pollenpreservation/chapter/prunus-pollen-cryopreservation/

Walnut pollen cryopreservation - (Book / Chapter)
Volk, G.M., Smith, J.L., Shepherd, A.N. 2025. Walnut pollen cryopreservation. In: Volk, G.M., Chen, K., editors. Pollen Preservation. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/pollenpreservation/chapter/walnut-pollen-cryopreservation/

Pecan pollen cryopreservation - (Book / Chapter)
Volk, G.M., Kubenka, K.A., Grauke, L.J., Chatwin, W.B., Shepherd, A.N. 2025. Pecan pollen cryopreservation. In: Volk, G.M., Chen, K., editors. Pollen Preservation. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/pollenpreservation/chapter/pecan-pollen-cryopreservation/

Pistachio pollen cryopreservation - (Book / Chapter)
Volk, G.M., Smith, J.L., Shepherd, A.N. 2025. Pistachio pollen cryopreservation. In: Volk, G.M., Chen, K., editors. Pollen Preservation. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/pollenpreservation/chapter/pistachio-pollen-cryopreservation/

Date palm pollen cryopreservation - (Book / Chapter)
Volk, G.M., Shepherd, A.N., Araújo De Oliveira, A.C., Krueger, R. 2025. Date palm pollen cryopreservation. In: Volk, G.M., Chen, K., editors. Pollen Preservation. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/pollenpreservation/chapter/date-palm-pollen-cryopreservation/

Using a vibrating microtome to prepare Mentha x piperita shoot tip sections for live-tissue microscopy experiments - (Peer Reviewed Journal)

Evaluating shoot-tip regrowth of 25 Rubus L. species and hybrids after 15 to 20 years of cryopreserved storage - (Peer Reviewed Journal)
Jenderek, M.M., Ambruzs, B.D., Yeater, K.M., Reed, B.M. 2024. Evaluating shoot-tip regrowth of 25 Rubus L. species and hybrids after 15 to 20 years of cryopreserved storage. Cryobiology. 118. Article e105159. https://doi.org/10.1016/j.cryobiol.2024.105159.

The USDA-ARS National Plant Germplasm System: Critical importance and impacts of long-term ex situ conservation - (Abstract Only)
Volk, G.M., Bretting, P.K. 2024. The USDA-ARS National Plant Germplasm System: Critical importance and impacts of long-term ex situ conservation [abstract]. X International Scientific and Practical Conference on Biotechnology as an Instrument for Plant Biodiversity Conservation. p. 26.

Analysis of drought risk index (DRI) and marker-trait associations (MTAs) for grain and mucilage traits in diverse Plantago species: Comparative analysis of MTAs by arithmetic and BLUP means - (Peer Reviewed Journal)
Shariari, Z., Heidari, B., Salami, M., Richards, C.M. 2024. Analysis of drought risk index (DRI) and marker-trait associations (MTAs) for grain and mucilage traits in diverse Plantago species: Comparative analysis of MTAs by arithmetic and BLUP means. Industrial Crops and Products. 222. Article e119699. https://doi.org/10.1016/j.indcrop.2024.119699.

Importance of the USDA-National Plant Germplasm System for climate-resilient agriculture - (Abstract Only)

Direct evidence that cryoprotectant mixtures facilitate individual component permeation into living plant cells - (Peer Reviewed Journal)
Pearce, K.C., Samuels, F., Volk, G.M., Levinger, N.E. 2024. Direct evidence that cryoprotectant mixtures facilitate individual component permeation into living plant cells. Cryobiology. 116. Article e104928. https://doi.org/10.1016/j.cryobiol.2024.104928.

The USDA-ARS National Plant Germplasm System strategic plan: A roadmap to conserve and utilize U.S. plant genetic resources - (Abstract Only)

Introduction to Crop Germplasm Committees: An opportunity to have an impact on crop collections in the USDA-ARS National Plant Germplasm System - (Abstract Only)

Development of a micropropagation protocol for the ex situ conservation of Nuttall’s scrub oak (Quercus dumosa) - (Peer Reviewed Journal)
Ree, J.F., Powell, C., Folgado, R., Pence, V.C., Walters, C.T., Maschinski, J. 2024. Development of a micropropagation protocol for the ex situ conservation of Nuttall’s scrub oak (Quercus dumosa). Plants. 13(8). Article e1148. https://doi.org/10.3390/plants13081148.

An introduction to the cryopreservation of clonally propagated plants - (Book / Chapter)
Bettoni, J.C., Chen, K., Volk, G.M. 2024. An introduction to the cryopreservation of clonally propagated plants. In: Volk, G.M., Chen, K., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/an-introduction-to-the-cryopreservation-of-clonally-propagated-plants/

Considerations when implementing shoot tip cryopreservation - (Book / Chapter)
Bettoni, J.C., Chen, K., Volk, G.M. 2024. Considerations when implementing shoot tip cryopreservation. In: Volk, G.M., Chen, K., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/considerations-when-implementing-shoot-tip-cryopreservation/

An overview of shoot tip cryopreservation - (Book / Chapter)
Bettoni, J.C., Chen, K., Volk, G.M. 2024. An overview of shoot tip cryopreservation. In: Volk, G.M., Chen, K., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/an-overview-of-shoot-tip-cryopreservation/

Cryotherapy using shoot tip cryopreservation - (Book / Chapter)
Bettoni, J.C., Volk, G.M. 2024. Cryotherapy using shoot tip cryopreservation. In: Volk, G.M., Chen, K., editors. Training in Plant Genetic Resources: Cryopreservation of Clonal Propagules. Fort Collins, Colorado: Colorado State University. Available: https://colostate.pressbooks.pub/clonalcryopreservation/chapter/an-introduction-to-the-cryopreservation-of-clonally-propagated-plants/

Embryos of Butia catarinensis are rudimentary and tolerant of desiccation and liquid nitrogen temperatures, but require GA3 to germinate - (Peer Reviewed Journal)
Groeten, D., Walters, C.T., Hill, L.M., Steiner, N. 2024. Embryos of Butia catarinensis are rudimentary and tolerant of desiccation and liquid nitrogen temperatures, but require GA3 to germinate. Plant Cell Tissue and Organ Culture. 156. Article e95. https://doi.org/10.1007/s11240-024-02717-5.

Evaluating seed longevity: Use of RNA integrity to characterize variation within species of legume grain - (Peer Reviewed Journal)
Tetreault, H.M., Redden, R.J., Fleming, M., Hill, L.M., Zingerman, Z., Whitehouse, K., Norton, S., Walters, C.T. 2024. Evaluating seed longevity: Use of RNA integrity to characterize variation within species of legume grain. Seed Science Research. https://doi.org/10.1017/S0960258524000072.

GRIN-U: Educational content about plant genetic resources - (Trade Journal)
Volk, G.M., Chen, K., Kinard, G.R., Gu, L., Byrne, P. 2024. GRIN-U: Educational content about plant genetic resources. Pomona. 57:16-18.

Workforce development and capacity-building: Applications of HRD to online training in plant genetic resources - (Abstract Only)

Germplasm of Ozark chinquapin (Castanea ozarkensis Ashe) can be cryopreserved by dormant winter buds - (Peer Reviewed Journal)
Jenderek, M.M., Yeater, K.M., Thomas, A.L. 2023. Germplasm of Ozark chinquapin (Castanea ozarkensis Ashe) can be cryopreserved by dormant winter buds. Cryobiology. 114. Article 104833. https://doi.org/10.1016/j.cryobiol.2023.104833.

Integration of genome-wide association studies, metabolomics, and transcriptomics reveals phenolic acid- and flavonoid-associated genes and their regulatory elements under drought stress in rapeseed flowers - (Peer Reviewed Journal)
Salami, M., Heidari, B., Batley, J., Wang, J., Tan, X., Richards, C.M., Tan, H. 2024. Integration of genome-wide association studies, metabolomics, and transcriptomics reveals phenolic acid- and flavonoid-associated genes and their regulatory elements under drought stress in rapeseed flowers. Frontiers in Plant Science. 14. Article e1249142. https://doi.org/10.3389/fpls.2023.1249142.

Genetic variation and response to selection of photosynthetic and forage characteristics in Kentucky bluegrass (Poa pratensis L.) ecotypes under drought conditions - (Peer Reviewed Journal)
Shariatipour, N., Shams, Z., Heidari, B., Richards, C.M. 2023. Genetic variation and response to selection of photosynthetic and forage characteristics in Kentucky bluegrass (Poa pratensis L.) ecotypes under drought conditions. Frontiers in Plant Science. 14. Article e1239860. https://doi.org/10.3389/fpls.2023.1239860.

Application of biotechniques for in vitro virus and viroid elimination in pome fruit crops - (Peer Reviewed Journal)
Bettoni, J.C., Wang, M., Li, J., Fan, X., Fazio, G., Hurtado-Gonzales, O.P., Volk, G.M., Wang, Q. 2024. Application of biotechniques for in vitro virus and viroid elimination in pome fruit crops. Phytopathology. 114(5):930-954. https://doi.org/10.1094/PHYTO-07-23-0232-KC.

Carbohydrate and dehydrin-like protein profiles during Araucaria angustifolia seed development provides insights toward ex situ conservation - (Peer Reviewed Journal)
Groeten, D., Farias-Soares, F., Rogge-Renner, G.D., Pereira, M.L., Walters, C.T., Silveira, V., Catarina, C.S., Guerra, M.P., Steiner, N. 2023. Carbohydrate and dehydrin-like protein profiles during Araucaria angustifolia seed development provides insights toward ex situ conservation. Trees. 37:1201-1215. https://doi.org/10.1007/s00468-023-02419-z.

Dissection of quantitative trait nucleotides and candidate genes associated with agronomic and yield-related traits under drought stress in rapeseed varieties: Integration of genome-wide association study and transcriptomic - (Peer Reviewed Journal)
Salami, M., Heidari, B., Alizadeh, B., Batley, J., Wang, J., Tan, X., Richards, C.M., Dadkhodaie, A. 2024. Dissection of quantitative trait nucleotides and candidate genes associated with agronomic and yield-related traits under drought stress in rapeseed varieties: Integration of genome-wide association study and transcriptomic analysis. Frontiers in Plant Science. 15. Article e1342359. https://doi.org/10.3389/fpls.2024.1342359.