Soluble extracts from carioca beans (Phaseolus vulgaris L.) affect the gut microbiota and iron related brush border membrane protein expression in vivo (Gallus gallus)

Authors: DIAS, DESIRRE - Federal University - Brazil; Kolba, Nikolai; NUTTI, MARILIA - Embrapa; MARTINO, HERCIA - Federal University - Brazil; Hart, Jonathan; MA, MICHELLE - Cornell University; LAKSHMANAN, NAVEENA - Cornell University; Glahn, Raymond; SHA, SYBIL - Cornell University; Tako, Elad

Submitted to: Food Research International
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/25/2019
Publication Date: 4/27/2019
Citation: Dias, D., Kolba, N.J., Nutti, M., Martino, H., Hart, J.J., Ma, M., Lakshmanan, N., Glahn, R.P., Sha, S., Tako, E.N. 2019. Soluble extracts from carioca beans (Phaseolus vulgaris L.) affect the gut microbiota and iron related brush border membrane protein expression in vivo (Gallus gallus). Food Research International. 123:172-180. https://doi.org/10.1016/j.foodres.2019.04.060.
DOI: https://doi.org/10.1016/j.foodres.2019.04.060

Interpretive Summary: Beans are a staple food crop consumed worldwide. In the current study, the effect of soluble extracts with potential prebiotic ability (i.e. improved gut health) extracted from various bean varieties and on the small intestinal functionality was evaluated by using the chicken animal model, and via the intra-amniotic administration procedure. Bean soluble extracts reduced the relative abundance of potential pathogenic bacterial groups, also, the expression of key intestinal proteins was upregulated by the tested extracts. Results suggested that soluble extracts from carioca beans may improve the intestinal functionality.

Technical Abstract: The effect of soluble extracts with putative prebiotic ability extracted from various bean varieties on the intestinal brush border membrane (BBM) iron related proteins, and intestinal bacterial populations were evaluated using the Gallus gallus model and by the intra-amniotic administration procedure. Eight treatment groups [(non-injected; 18'MO H2O; 40'mg/mL Inulin; 50'mg/mL BRS Perola (carioca standard); 50'mg/mL BRS Cometa (carioca, Fe biofortified); 50'mg/mL BRS Esteio (black, standard); 50'mg/mL SMN 39 (black, Fe biofortified); 50'mg/mL BRS Artico (white, standard)] were utilized. Tested groups reduced the relative abundance of Clostridium and E. coli compared to the Inulin group (positive control) and they did not affect the relative abundance of Bifidobacterium and Lactobacillus compared to the negative control (18MO H2O). The relative expression of zinc transporter 1, ferroportin and amino peptidase were up-regulated in the BRS Cometa group (Fe-biofortified carioca beans). Results suggest that soluble extracts from carioca beans may improve the iron bioavailability by affecting intestinal bacterial populations, and BBM functionality.