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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Characterization and Interventions for Foodborne Pathogens » Research » Publications at this Location » Publication #368933
Research Project: Shiga Toxin-Producing Escherichia coli in Biofilms and within Microbial Communities in Food

Location: Characterization and Interventions for Foodborne Pathogens

Title: Incidence and characterization of Salmonella from raw meat products sold at small markets in Hubei province China

Author
item ZHOU, MIN - Wuhan Institute Of Technology
item LI, XIAOFANG - Wuhan Institute Of Technology
item HOU, WENFU - Wuhan Institute Of Technology
item WANG, HONGXUN - Wuhan Institute Of Technology
item Paoli, George
item SHI, XIANMING - Shanghai Jiaotong University

Submitted to: Frontiers in Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/17/2019
Publication Date: 10/4/2019
Citation: Zhou, M., Li, X., Hou, W., Wang, H., Paoli, G., Shi, X. 2019. Incidence and characterization of Salmonella from raw meat products sold at small markets in Hubei province China. Frontiers in Microbiology. 10(2265). Available: https://doi.org/10.3389/fmicb.2019.02265l. https://doi.org/10.3389/fmicb.2019.02265.
DOI: https://doi.org/10.3389/fmicb.2019.02265

Interpretive Summary: Salmonella is a leading cause of foodborne disease and is often associated with the consumption of foods of animal origin. In this study an ARS scientist in Wyndmoor, PA collaborated with Chinese scientists to determine the incidence of Salmonella and the antibiotic resistance profiles of Salmonella isolates from a variety of raw meat samples. Sixty-six Salmonella isolates were obtained from 631 raw meat samples purchased at small retail suppliers in Hubei Province, China. More than three quarters of the isolates were resistant to at least on antibiotic and more and half were resistant to multiple antibiotics. In addition, genetic factors for the movement of the antibiotic resistance genes were identified. This study sets a baseline for the regional incidence of Salmonella in raw meats and provides evidence for mechanisms of antibtic resistance and mobility of antibiotic resistance genes.

Technical Abstract: Salmonella is a leading cause of foodborne disease and is often associated with the consumption of foods of animal origin. In this study, 66 Salmonella isolates were obtained from 631 raw meat samples purchased at small retail suppliers in Hubei Province, China. The most prevalent Salmonella serotypes were Thompson (18.2%) and Agona (13.6%). Frequent antimicrobial resistance was observed for the sulphonamides (43.9%), tetracycline (43.9%), and the ß-lactams amoxicillin and ampicillin (36.4% for each). Interestingly, a high incidence of resistance to cephazolin was observed in strains of the most common serotype, S. Thompson. Class I integrons were found in 27.3% (18/66) of the isolates and these isolates contained 5 different gene cassettes (aacA4C-arr-3-dfr2, dfrA12-aadA21, aadA2, dfrA12-aadA2, dfr17-aadA5). Additional antimicrobial resistance genes including blaTEM--1, blaCTX-M-65, blaCTX-M-15, qnrB and qnrS were detected among the Salmonella isolates. Results of replicon typing and conjugation experiments revealed that an integron with qnrB and blaCTX-M-15 genes was present on incH12 plasmids in S. Thompson. Multilocus sequence typing analysis revealed 32 sequence types, indicating that these isolates were phenotypically and genetically diverse. ST26 (18.2%) and ST541 (12.1%) were the predominant sequence types. The integrons along with multiple antibiotic resistance genes on mobile plasmids are likely contributors to the dissemination of multidrug resistance in Salmonella.