Ilenys Perez Diaz
(PhD)
Food Science and Market Quality and Handling Research Unit
Microbiologist
Phone: (919) 513-0165
Fax: (919) 513-0180
(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)
Publications
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Enhancement of gamma-aminobutyric acid in fermented cucumbers
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Annotated whole-genome sequences of fermentative and spoilage associated bacilli and proteobacteria autochthonous to commercial cucumber fermentation
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Page, C.A., Simpson, S.A., Perez Diaz, I.M., Rivers, A.R. 2024. Annotated whole-genome sequences of fermentative and spoilage associated bacilli and proteobacteria autochthonous to commercial cucumber fermentation. Microbiology Resource Announcements. 13. Article e00926-23. https://doi.org/10.1128/mra.00926-23.
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CO2-mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation
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Zhai, Y., Pagan Medina, C.G., Perez Diaz, I.M. 2023. CO2-mediated bloater defect can be induced by the uncontrolled growth of Enterobacteriaceae in cucumber fermentation. Food Science and Nutrition. 11(10):6178-6187. https://doi.org/10.1002/fsn3.3557.
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Levilactobacillus brevis autochthonous to cucumber fermentation is unable to utilize citric acid and encodes for a putative 1,2-propanediol utilization microcompartment
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Perez Diaz, I.M., Page, C.A., Mendez-Sandoval, L., Johanningsmeier, S.D. 2023. Levilactobacillus brevis autochthonous to cucumber fermentation is unable to utilize citric acid and encodes for a putative 1,2-propanediol utilization microcompartment. Frontiers in Microbiology. 14:1210190. https://doi.org/10.3389/fmicb.2023.1210190.
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Whole-genome sequencing and annotation of selected Enterobacteriaceae derived from commercial cucumber fermentation
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Page, C.A., Sattar, A., Perez Diaz, I.M. 2023. Whole-genome sequencing and annotation of selected Enterobacteriaceae derived from commercial cucumber fermentation. Microbiology Resource Announcements. 12(9). Article e00295-23. https://doi.org/10.1128/MRA.00295-23.
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Genome-wide comparative analysis of Lactiplantibacillus pentosus isolates autochthonous to cucumber fermentation reveals subclades of divergent ancestry
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Page, C.A., Perez Diaz, I.M., Pan, E., Barrangou, R. 2023. Genome-wide comparative analysis of Lactiplantibacillus pentosus isolates autochthonous to cucumber fermentation reveals subclades of divergent ancestry. Foods. 12(13):2455. https://doi.org/10.3390/foods12132455.
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Whole-genome sequencing and annotation of Pediococcus ethanolidurans and Pediococcus pentosaceous isolates from commercial cucumber fermentation
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Page, C.A., Perez Diaz, I.M. 2023. Whole-genome sequencing and annotation of Pediococcus ethanolidurans and Pediococcus pentosaceous isolates from commercial cucumber fermentation. Microbiology Resource Announcements. 12(5):e00050-23. https://doi.org/10.1128/mra.00050-23.
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Prevention of microbes-induced spoilage in sodium chloride-free cucumber fermentations employing preservatives
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Perez Diaz, I.M., Medina, E., Page, C.A., Johanningsmeier, S.D., Daughtry, K.V., Moeller, L. 2022. Prevention of microbes-induced spoilage in sodium chloride-free cucumber fermentations employing preservatives. Journal of Food Science. 87(11):5054-5069. https://doi.org/10.1111/1750-3841.16345.
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Genome sequences for Levilactobacillus brevis autochthonous to commercial cucumber fermentations
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Page, C.A., Carter-Ogden, R., Lee, A.M., Perez Diaz, I.M. 2022. Genome sequences for Levilactobacillus brevis autochthonous to commercial cucumber fermentations. Microbiology Resource Announcements. 11(5):e00029-22. https://doi.org/10.1128/mra.00029-22.
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Growth of gamma-proteobacteria in cucumber fermentation is prevented by lactobacilli and the cover brine ingredients
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Rothwell, M.A., Zhai, Y., Pagan Medina, C., Perez Diaz, I.M. 2022. Growth of gamma-proteobacteria in cucumber fermentation is prevented by lactobacilli and the cover brine ingredients. Microbiology Spectrum. 10(3):e01031-21. https://doi.org/10.1128/spectrum.01031-21.
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Vegetable fermentations brined with low salt for reclaiming food waste
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Little, C., Cruz-Martínez, V., St. Fort, D.P., Pagán-Medina, C., Page, C.A., Perez-Perez, Y., Taveirne, M., Lee, A., Arroyo González, N., Santiago Ortiz, C., Perez Diaz, I.M. 2022. Vegetable fermentations brined with low salt for reclaiming food waste. Journal of Food Science. 87(5):2121-2132. https://doi.org/10.1111/1750-3841.16084.
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Whole-genome sequencing and annotation of selected Lactobacillales isolated from commercial cucumber fermentation
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Perez Diaz, I.M., Page, C.A. 2021. Whole-genome sequencing and annotation of selected Lactobacillales isolated from commercial cucumber fermentation. Microbiology Resource Announcements. 10(43):e00625-21. https://doi.org/10.1128/MRA.00625-21.
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Identification of potential causative agents of the CO2-mediated bloater defect in low salt cucumber fermentation
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Zhai, Y., Perez-Diaz, I.M. 2021. Identification of potential causative agents of the CO2-mediated bloater defect in low salt cucumber fermentation. International Journal of Food Microbiology. 344:109115. https://doi.org/10.1016/j.ijfoodmicro.2021.109115.
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Genotypic and phenotypic diversity among Lactobacillus plantarum and Lactobacillus pentosus isolated from industrial scale cucumber fermentations
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Perez Diaz, I.M., Johanningsmeier, S.D., Anekella, K., Pagan Medina, C.G., Mendez-Sandoval, L., Arellano, C., Price, R., Daughtry, K.V., Borges, M., Bream, C., Connelly, L., Dieck, S.E., Levi, M.T., Mcmurtrie, E.K., Smith, R.E., Theora, J.C., Wendland, P., Gomez-Rodriguez, F., Arroyo-Lopez, F. 2021. Genotypic and phenotypic diversity among Lactobacillus plantarum and Lactobacillus pentosus isolated from industrial scale cucumber fermentations. Food Microbiology. 94:103652. https://doi.org/10.1016/j.fm.2020.103652.
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Gentiobiose and cellobiose content in fresh and fermenting cucumbers and utilization of such disaccharides by lactic acid bacteria in fermented cucumber juice medium
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Ucar, R.A., Perez Diaz, I.M., Dean, L.L. 2020. Gentiobiose and cellobiose content in fresh and fermenting cucumbers and utilization of such disaccharides by lactic acid bacteria in fermented cucumber juice medium. Food Science and Nutrition. 8(11):5798-5810. https://doi.org/10.1002/fsn3.1830.
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Characterization of robust Lactobacillus plantarum and Lactobacillus pentosus starter cultures for environmentally friendly low-salt cucumber fermentations
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Anekella, K., Perez Diaz, I.M. 2020. Characterization of robust Lactobacillus plantarum and Lactobacillus pentosus starter cultures for environmentally friendly low-salt cucumber fermentations. Journal of Food Science. 85(10):3487-3467. https://doi.org/10.1111/1750-3841.15416.
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Bacteriophages infecting gram-negative bacteria in a commercial cucumber fermentation
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Lu, Z., Perez Diaz, I.M., Hayes, J., Breidt, F. 2020. Bacteriophages infecting gram-negative bacteria in a commercial cucumber fermentation. Frontiers in Microbiology. 11:1306. https://doi.org/10.3389/fmicb.2020.01306.
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Contribution of Leuconostocaceae to CO2-mediated bloater defect in cucumber fermentation
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Zhai, Y., Perez Diaz, I.M. 2020. Contribution of Leuconostocaceae to CO2-mediated bloater defect in cucumber fermentation. Food Microbiology. 91:103536. https://doi.org/10.1016/j.fm.2020.103536.
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Isolation of exopolysaccharide-producing yeast and lactic acid bacteria from quinoa (Chenopodium quinoa) sourdough fermentation
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Franco, W., Perez Diaz, I.M., Connelly, L.E., Diaz, J. 2020. Isolation of exopolysaccharide-producing yeast and lactic acid bacteria from quinoa (Chenopodium quinoa) sourdough fermentation. Foods. 9(3):337. https://doi.org/10.3390/foods9030337.
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Content of xylose, trehalose and L-citrulline in cucumber fermentations and utilization of such compounds by certain lactic acid bacteria
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Ucar, R.A., Perez Diaz, I.M., Dean, L.L. 2020. Content of xylose, trehalose and L-citrulline in cucumber fermentations and utilization of such compounds by certain lactic acid bacteria. Food Microbiology. 91:103454. https://doi.org/10.1016/j.fm.2020.103454.
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Modulation of the bacterial population in commercial cucumber fermentations by brining salt type
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Perez Diaz, I.M., Dickey, A., Fitria, R., Ravishankar, N., Hayes, J.S., Campbell, K., Arritt, F. 2020. Modulation of the bacterial population in commercial cucumber fermentations by brining salt type. Journal of Applied Microbiology. 128(6):1678-1693. https://doi.org/10.1111/jam.14597.
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Fermented vegetables as vectors for the relocation of microbial diversity from the environment to the human gut
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Perez Diaz, I.M. 2019. Fermented vegetables as vectors for the relocation of microbial diversity from the environment to the human gut. Book Chapter. p 91-120. https://doi.org/10.1007/978-3-030-28737-5_4.
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Assessment of the non-lactic acid bacteria microbiota in fresh cucumbers and commercially fermented cucumber pickles brined with 6% NaCl
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Perez Diaz, I.M., Hayes, J.S., Medina, E., Webber, A., Butz, N., Dickey, A., Lu, Z., Azcarate-Peril, M.A. 2019. Assessment of the non-lactic acid bacteria microbiota in fresh cucumbers and commercially fermented cucumber pickles brined with 6% NaCl. Food Microbiology. 77:10-20. https://doi.org/10.1016/j.fm.2018.08.003.
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Viability of commercial cucumber fermentation without nitrogen or air purging
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Zhai, Y., Perez Diaz, I.M., Diaz, J.T. 2018. Viability of commercial cucumber fermentation without nitrogen or air purging. Trends in Food Science and Technology. 81:185-192. https://doi.org/10.1016/j.tifs.2018.05.017.
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Physical properties of NaCl-free cucumber fermentation cover brine containing calcium chloride and glycerin and apparent freezing injury of the brined fruits
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Diaz, J.T., Perez Diaz, I.M., Messer, N., Safferman, S.I. 2018. Physical properties of NaCl-free cucumber fermentation cover brine containing calcium chloride and glycerin and apparent freezing injury of the brined fruits. Journal of Food Processing and Preservation. 42(4):e13582. https://doi.org/10.1111/jfpp.13582.
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Evaluation of the use of malic acid decarboxylase-deficient starter culture in NaCl-free cucumber fermentations to reduce bloater incidence
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Zhai, Y., Perez Diaz, I.M., Diaz, J., Lombardi, R.L., Connelly, L.E. 2018. Evaluation of the use of malic acid decarboxylase-deficient starter culture in NaCl-free cucumber fermentations to reduce bloater incidence. Journal of Applied Microbiology. 124(1):197-208. https://doi.org/10.1111/jam.13625.
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Fermentation cover brine reformulation for cucumber processing with low salt to reduce bloater defect
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Zhai, Y., Perez Diaz, I.M. 2017. Fermentation cover brine reformulation for cucumber processing with low salt to reduce bloater defect. Journal of Food Science. 82(12):2987-2996. https://doi.org/10.1111/j.1750-3841.2010.01558.x.
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Microbial fermentation in food preservation
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Perez Diaz, I.M., Gunes Altuntas, E., Juneja, V.K. 2018. Microbial fermentation in food preservation. In: Juneja, V.K., Dwivedi, H.P., Sofos, J.N., editors. Microbial Control and Food Preservation. New York, NY: Springer. p. 281-298. https://doi.org/10.1007/978-1-4939-7556-3_13.
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Winterization strategies for bulk storage of pickles
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Diaz, J.T., Perez Diaz, I.M., Simunovic, J., Sandeep, K.P. 2017. Winterization strategies for bulk storage of pickles. Journal of Food Engineering. 212:12-17. https://doi.org/10.1016/j.jfoodeng.2017.03.027.
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Reassessment of the succession of lactic acid bacteria in commercial cucumber fermentations and physiological and genomic features associated with their dominance
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Perez Diaz, I.M., Hayes, J.S., Medina-Pradas, E., Anekella, K., Daughtry, K.V., Dieck, S., Levi, M., Price, R., Butz, N., Lu, Z., Azcarte-Peril, M. 2017. Reassessment of the succession of lactic acid bacteria in commercial cucumber fermentations and physiological and genomic features associated with their dominance. Food Microbiology. 63:217-227. https://doi.org/10.1016/j.fm.2016.11.025.
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Survival and growth of probiotic lactic acid bacteria in refrigerated pickle products
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Fan, S., Breidt, F., Price, R.E., Perez Diaz, I.M. 2017. Survival and growth of probiotic lactic acid bacteria in refrigerated pickle products. Journal of Food Science. 82(1):167-173.
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Review of vegetable fermentations with particular emphasis on processing modifications, microbial ecology, and spoilage
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Medina Pradas, E., Perez Diaz, I.M., Garrido-Fernandez, A., Arroyo-Lopez, F.N. 2016. Review of vegetable fermentations with particular emphasis on processing modifications, microbial ecology, and spoilage. In: Bevilacqua, A., Rosaria Corbo, M., Sinigaglia, M., editors. The Microbiological Quality of Food. Cambridge, MA: Woodhead Publishing. p. 211-236.
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Bacterial ecology of fermented cucumber rising pH spoilage as determined by non-culture based methods
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Medina-Pradas, E., Perez Diaz, I.M., Breidt, F., Hayes, J.S., Franco, W., Butz, N., Azcarate-Peril, A. 2016. Bacterial ecology of fermented cucumber rising pH spoilage as determined by non-culture based methods. Journal of Food Science. 80(1):M121-M129. doi: 10.1111/1750-3841.13158.
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Commercial scale cucumber fermentations brined with calcium chloride instead of sodium chloride
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Perez Diaz, I.M., McFeeters, R.F., Moeller, L., Johanningsmeier, S.D., Hayes, J.S., Fornea, D., Gilbert, C., Custis, N., Beene, K., Bass, D. 2015. Commercial scale cucumber fermentations brined with calcium chloride instead of sodium chloride. Journal of Food Science. 80(12):M2827-M2836. doi: 10.1111/1750-3841.13107.
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Characterization of the microbial diversity in yacon spontaneous fermentation at 20 ºC
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Reina, L., Perez Diaz, I.M., Breidt, F., Azcarate-Peril, M., Medina, E., Butz, N. 2015. Characterization of the microbial diversity in yacon spontaneous fermentation at 20 ºC. International Journal of Food Microbiology. 203:35-40. 10.1016/j.ijfoodmicro.2015.03.007.
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Fermented and Acidified Vegetables
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Perez Diaz, I.M., Breidt, F., Buescher, R.W., Arroyo-Lopez, F.N., Jimenez-Diaz, R., Bautista-Gallego, J., Garrido-Fernandez, A., Yoon, S., Johanningsmeier, S.D. 2014. Fermented and Acidified Vegetables. Compendium of Methods for the Microbiological Examination of Foods. Chapter 51.
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Characterization of cucumber fermentation spoilage bacteria by enrichment culture and 16S rDNA cloning
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Breidt, F., Medina-Pradas, E., Wafa, D., Perez Diaz, I.M., Franco, W., Huang, H., Johanningsmeier, S.D., Kim, J. 2013. Characterization of cucumber fermentation spoilage bacteria by enrichment culture and 16S rDNA cloning. Journal of Food Science. 78(3):M470-M476.
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Putative and unique gene sequence utilization for the design of species specific probes as modeled by Lactobacillus plantarum
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Perez Diaz, I.M. 2013. Putative and unique gene sequence utilization for the design of species specific probes as modeled by Lactobacillus plantarum. Current Microbiology. 66:266-270.
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Bacteriophage ecology in a commercial cucumber fermentation
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Lu, Z., Perez Diaz, I.M., Hayes, J.S., Breidt, F. 2012. Bacteriophage ecology in a commercial cucumber fermentation. Applied and Environmental Microbiology. 78(24):8571-8578.
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Microbial interactions associated with secondary cucumber fermentation
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Perez-Diaz, I.M., Franco, W. 2013. Microbial interactions associated with secondary cucumber fermentation. Journal of Applied Microbiology. 114:161-172.
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Development of an effective treatment for a 5-log reduction of Escherichia coli in refrigerated pickle products
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Lu, H.J., Breidt, F., Perez-Diaz, I.M. 2013. Development of an effective treatment for a 5-log reduction of Escherichia coli in refrigerated pickle products. Journal of Food Science. 78(2):M264-M269.
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Role of selected oxidative yeasts and bacteria in cucumber secondary fermentation associated with spoilage of the fermented fruit
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Perez Diaz, I.M., Franco, W. 2012. Role of selected oxidative yeasts and bacteria in cucumber secondary fermentation associated with spoilage of the fermented fruit. Food Microbiology. 32:338-344.
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Development of a model system for the study of spoilage associated secondary cucumber fermentation during long term storage
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Franco, W., Perez-Diaz, I.M. 2012. Development of a model system for the study of spoilage associated secondary cucumber fermentation during long term storage. Journal of Food Science. 77(10):M586-M592.
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Influence of sodium chloride, pH, and lactic acid bacteria on anaerobic lactic acid utilization during fermented cucumber spoilage
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Johanningsmeier, S.D., Franco, W., Perez Diaz, I.M., McFeeters, R.F. 2012. Influence of sodium chloride, pH, and lactic acid bacteria on anaerobic lactic acid utilization during fermented cucumber spoilage. Journal of Food Science. 77(7):M397-M404.
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Characteristics of spoilage-associated secondary cucumber fermentation
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Franco, W., Perez-Diaz, I.M., Johanningsmeier, S.D., McFeeters, R.F. 2012. Characteristics of spoilage-associated secondary cucumber fermentation. Applied and Environmental Microbiology. 78(4):1273-1284.
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Growth of Lactobacillus paracasei ATCC334 in a cheese model system: A biochemical approach
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Budinich, M.F., Perez Diaz, I.M., Cai, H., Rankin, S.A., Broadbent, J.R., Steele, J.L. 2011. Growth of Lactobacillus paracasei ATCC334 in a cheese model system: A biochemical approach. Journal of Dairy Science. 94(11):5263-5277.
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Preservation of acidified cucumbers with a combination of fumaric acid and cinnamaldehyde that target lactic acid bacteria and yeasts
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Perez Diaz, I.M. 2011. Preservation of acidified cucumbers with a combination of fumaric acid and cinnamaldehyde that target lactic acid bacteria and yeasts. Journal of Food Science. 76(7):M473-M477.
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Antimicrobial effects of weak acids on the survival of Escherichia coli O157:H7 under anaerobic conditions
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Lu, H.J., Breidt, F., Perez-Diaz, I.M., Osborne, J.A. 2011. Antimicrobial effects of weak acids on the survival of Escherichia coli O157:H7 under anaerobic conditions. Journal of Food Protection. 6:893-898.
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Fermented Vegetables
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Breidt, F., McFeeters, R.F., Perez Diaz, I.M., Lee, C. 2012. Fermented Vegetables. In: Doyle, M.P., Buchanan, R.L., editors. Food Microbiology: Fundamentals and Frontiers, 4th Ed. Washington, D.C.: ASM Press. p. 841-855.
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Preparation of a Lactobacillus plantarum starter culture for cucumber fermentations that can meet kosher guidelines
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Perez Diaz, I.M., McFeeters, R.F. 2011. Preparation of a Lactobacillus plantarum starter culture for cucumber fermentations that can meet kosher guidelines. Journal of Food Science. 76(2):M120-M123.
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Preservation of acidified cucumbers with a natural preservative combination of fumaric acid and allyl isothiocyanate that target lactic acid bacteria and yeasts
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Perez Diaz, I.M., Mcfeeters, R.F. 2010. Preservation of acidified cucumbers with a natural preservative combination of fumaric acid and allyl isothiocyanate that target lactic acid bacteria and yeasts. Journal of Food Science. 75(4):M204-M208.
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Fermentation of cucumbers brined with calcium chloride instead of sodium chloride
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McFeeters, R.F., Perez Diaz, I.M. 2010. Fermentation of cucumbers brined with calcium chloride instead of sodium chloride. Journal of Food Science. 75(3):C291-C296.
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Influence of microbial growth in the redox potential of fermented cucumbers
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Olsen, M., Perez Diaz, I.M. 2009. Influence of microbial growth in the redox potential of fermented cucumbers. Journal of Food Science. 74(4):M149-M153.
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Modification of azo dyes by lactic acid bacteria
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Perez Diaz, I.M., McFeeters, R.F. 2009. Modification of azo dyes by lactic acid bacteria. Journal of Applied Microbiology. 107:584-589.
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Microbiological preservation of cucumbers for bulk storage by the use of acetic acid and food preservatives
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Perez Diaz, I.M., McFeeters, R.F. 2008. Microbiological preservation of cucumbers for bulk storage by the use of acetic acid and food preservatives. Journal of Food Science. 73(6):M287-M291.
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Microbial growth and the effects of mild acidification and preservatives in refrigerated sweetpotato puree
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Perez Diaz, I.M., Truong, V., Webber, A.M., McFeeters, R.F. 2008. Microbial growth and the effects of mild acidification and preservatives in refrigerated sweetpotato puree. Journal of Food Protection. 71(3):639-642.
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Lactobacilli and tartrazine as causative agents of a red colored spoilage in cucumber pickle products
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Perez Diaz, I.M., Kelling, R., Hale, S., Breidt, F., McFeeters, R.F. 2007. Lactobacilli and tartrazine as causative agents of a red colored spoilage in cucumber pickle products. Journal of Food Science. 72(7):M240-M245.
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Fermented Vegetables
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Breidt, Jr., F., McFeeters, R. F., and Diaz-Muniz, I. 2007. Fermented Vegetables. In: Food Microbiology: Fundamentals and Frontiers, 3rd Ed. M. P. Doyle and L. R. Beuchat, eds, ASM Press, Washington, D.C. p. 783-793
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Comparative genomics of the lactic acid bacteria
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Makarova, K., Slesarev, A., Wolf, Y., Sorokin, A., Mirkin, B., Koonin, E., Pavlov, A., Pavlova, N., Karamychev, V., Polouchine, N., Shakhova, V., Grigoriev, I., Lou, Y., Rohksar, D., Lucas, S., Huang, K., Goodstein, D.M., Hawkins, T., Plengvidhya, V., Welker, D., Hughes, J., Goh, Y., Benson, A., Baldwin, K., Lee, J.H., Perez Diaz, I.M., Dosti, B., Smeianov, V., Wechter, W.P., Barabote, R., Lorca, G., Altermann, E., Barrangou, R., Ganesan, B., Xie, Y., Rawsthorne, H., Tamir, D., Parker, C., Breidt, F., Broadbent, J., Hutkins, R., O'Sullivan, D., Steele, J., Unlu, G., Saier, M., Klaenhammer, T., Richardson, P., Kozyavkin, S., Weimer, B., Mills, D. 2006. Comparative genomics of the lactic acid bacteria. Proceedings of the National Academy of Sciences. 103(42):15611-15616.
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LIMITING GALACTOSE REQUIREMENT FOR CITRATE UTILIZATION BY LACTOBACILLUS CASEI IS ANNULLED IN CHEDDAR CHEESE EXTRACT
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LACTOBACILLUS CASEI METABOLIC POTENTIAL TO UTILIZE CITRATE AS AN ENERGY SOURCE IN RIPENING CHEESE: A BIOINFORMATICS APPROACH
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ARS News Articles
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