Research Project: Strategies to Reduce Mycotoxin Contamination in Animal Feed and its Effect in Poultry Production Systems

Location: Toxicology & Mycotoxin Research

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Comparison of different levels of iron and zinc for enriched eggs in layers - (Peer Reviewed Journal)
Ullah, Z., Khan, S., Shuaib, M., Iqbal, A., Siddiqui, S.A., Pokoo-Aikins, A., Swelum, A.A. 2024. Comparison of different levels of iron and zinc for enriched eggs in layers. The Veterinary Quarterly. Volume 44, 2024 - Issue 1, pages 1-7. https://doi.org/10.1080/01652176.2024.2431035.

Mycotoxin contamination and the nutritional content of corn targeted for animal feed - (Peer Reviewed Journal)
Pokoo-Aikins, A., Mcdonough, C., Mitchell, T.R., Hawkins, J.A., Adams, L.F., Read, Q.D., Li, X., Shanmugasundaram, R., Rodewald, E., Acharya, P., Glenn, A.E., Gold, S.E. 2024. Mycotoxin contamination and the nutritional content of corn targeted for animal feed. Poultry Science. Volume 103, Issue 12. https://doi.org/10.1016/j.psj.2024.104303.

Effect of multi-strain probiotics on production performance, gut health and nutrient utilization of broilers fed poultry by-products meal diet. - (Peer Reviewed Journal)
Ahmad, U., Sultan, A., Shuaib, M., Atif, M., Alqhtani, A.H., Pokoo-Aikins, A., Ullah, O., Siddiqui, S.A. 2024. Effect of multi-strain probiotics on production performance, gut health and nutrient utilization of broilers fed poultry by-products meal diet.. Italian Journal of Animal Science. 23, 1250-1257. https://doi.org/10.1080/1828051X.2024.2391084.

A Fusarium verticillioides MAT1-2 strain near isogenic to the sequenced FGSC7600 strain for producing homozygous multigene mutants - (Peer Reviewed Journal)
Gold, S.E., Brown, D.W., Williams, F.N., Naden, B.D., Vo, V., Miller, C.E. 2024. A Fusarium verticillioides MAT1-2 strain near isogenic to the sequenced FGSC7600 strain for producing homozygous multigene mutants. The Journal of Fungi. 10, 592. https://doi.org/10.3390/jof10080592.

Growth performance and immune response during active Eimeria infection are modified by dietary inclusion of canola meal or corn-DDGS in reduced-protein corn-soybean meal diets - (Peer Reviewed Journal)
Shanmugasundaram, R., Adeleye, A.M., Fathima, S., Oladeinde, A., Selvaraj, R., Applegate, T.J., Olukosi, O.A. 2024. Growth performance and immune response during active Eimeria infection are modified by dietary inclusion of canola meal or corn-DDGS in reduced-protein corn-soybean meal diets.. Animal Nutrition. (19); 1-15. https://doi.org/10.1016/j.aninu.2024.05.007.

Effect of 125% and 135% arginine on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge - (Peer Reviewed Journal)
Fathima, S., Hakeem, W., Shanmugasundaram, R., Periyannan, V., Varadhan, R., Selvaraj, R. 2024. Effect of 125% and 135% arginine on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge. Poultry Science. 103(7):103815. https://doi.org/10.1016/j.psj.2024.103826.

Effects of Subclinical Mycotoxin Exposure and Coccidial Challenge on Growth Performance, Body Composition, Gut Health, and Egg Production During Pullet-to-Layer Transition Period - (Abstract Only)

Effects of Mycotoxin-Contaminated Corn Distillers’ Dried Grains with Solubles on Growth Performance, Gut Health, and Immune Function in Four- to Eight-Week Old Pullets - (Abstract Only)

Interactions of coccidial vaccination status and protein sources in reduced-protein diets: impacts on immune responses and growth performance in broiler chickens - (Abstract Only)

MicroRNAs as Early Biomarkers to Identify Fumonisins, Deoxynivalenol, and Zearalenone Exposure in Broiler Chickens - (Abstract Only)

Effect of synbiotic supplementation on growth performance and immune response of broiler chickens exposed to fumonisins, Type B trichothecenes, and zearalenone - (Abstract Only)

Impact of mycotoxin deactivator on immune response and growth Performance in broiler chickens exposed to Type B trichothecenes, fumonisins, and zearalenone - (Abstract Only)

Understanding and Combating Mycotoxins for Sustainable Poultry Production - (Abstract Only)

Effect of arginine supplementation on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge. - (Peer Reviewed Journal)
Fathima, S., Hakeem, W., Shanmugasundaram, R., Selvaraj, R. 2024. Effect of arginine supplementation on the growth performance, intestinal health, and immune responses of broilers during necrotic enteritis challenge.. Poultry Science. 103: 7, 103815. https://doi.org/10.1016/j.psj.2024.103815.

Effects of dietary supplementation of purslane (portulaca oleraceae), prebiotics, and probiotics on breast meat quality of broilers - (Abstract Only)
Noh, E., Pokoo-Aikins, A., Jones, M., Mwangi, S.N., Min, B.R. 2024. Effects of dietary supplementation of purslane (portulaca oleraceae), prebiotics, and probiotics on breast meat quality of broilers. American Society of Animal Science Annual Meeting. 102, 529-530. https://doi.org/10.1093/jas/skae234.595..

MicroRNAs: Exploring their role in farm animal disease and mycotoxin challenges - (Review Article)
Kappari, L., Joseph, D.R., Applegate, T., Selvaraj, R., Shanmugasundaram, R. 2024. MicroRNAs: Exploring their role in farm animal disease and mycotoxin challenges. Frontiers in Veterinary Science. 11: 1372961. https://doi.org/10.3389/fvets.2024.1372961.

Supplementing low protein diets with methionine or threonine during mixed Eimeria challenge - (Peer Reviewed Journal)
Taylor, J., Mercier, Y., Olukosi, O., Kim, W.K., Selvaraj, R., Applegate, T., Shanmugasundaram, R., Ball, E., Kyriazakis, I. 2024. Supplementing low protein diets with methionine or threonine during mixed Eimeria challenge. Poultry Science. 103: (6); 103714. https://doi.org/10.1016/j.psj.2024.103714.

Microbiota profile and antibiotic resistance genes in the caecal content of broiler chickens are influenced by dietary protein content and the plant protein source - (Abstract Only)

Effects of soybean meal and wheat bran on immune response and cecal bacterial antimicrobial resistance gene response in broilers - (Abstract Only)

Characterizing the effect of Campylobacter jejuni challenge on growth performance, cecal microbiota, and cecal short-chain fatty acid concentrations in broilers - (Peer Reviewed Journal)
Hakeem, W.G., Cason, E.E., Adams, D., Fathima, S., Shanmugasundaram, R., Lourenco, J., Selvaraj, R.K. 2024. Characterizing the effect of Campylobacter jejuni challenge on growth performance, cecal microbiota, and cecal short-chain fatty acid concentrations in broilers. Animals. 14(3),473. https://doi.org/10.3390/ani14030473.

The effect of Campylobacter jejuni challenge on the ileal microbiota and short-chain fatty acids at 28 and 35 days of age - (Peer Reviewed Journal)
Hakeem, W., Cason, E.E., Villanueva, K.A., Shanmugasundaram, R., Lourenco, J., Selvaraj, R. 2024. The effect of Campylobacter jejuni challenge on the ileal microbiota and short-chain fatty acids at 28 and 35 days of age. Italian Journal of Animal Science. VOL. 23, NO. 1, 299–312. https://doi.org/10.1080/1828051X.2024.2310588.

Effect of arginine supplementation on the production, intestinal health, and immune response of broilers during necrotic enteritis challenge - (Abstract Only)

In vitro Dose-Response Effects of Fumonisin B1 and Deoxynivalenol on the HD-11 Macrophage Cell Line Following Lipopolysaccharide Challenge - (Abstract Only)

Effect of Sub-Clinical Mycotoxin Exposure on Growth Performance and Immune Response in Eimeria-Challenged Pullets - (Abstract Only)

Optimizing protein sources in reduced-protein diets to improve the immune responses during coccidiosis in broiler chickens - (Abstract Only)

Beyond protein synthesis: The emerging role of arginine in poultry nutrition and host-microbe interactions - (Review Article)
Fathima, S., Hakeem, W., Selvaraj, R., Shanmugasundaram, R. 2024. Beyond protein synthesis: The emerging role of arginine in poultry nutrition and host-microbe interactions. Frontiers in Physiology. Volume 14(2024),1326809. https://doi.org/10.3389/fphys.2023.1326809.

Effect of supplementation of papaya seed (Carica papaya) on growth performance, carcass traits, and histomorphology of Japanese quails - (Peer Reviewed Journal)
Ullah, I., Ullah, R., Shuaib, M., Sohaib, H.U., Alqhtani, A.H., Pokoo-Aikins, A., Alam, W., Siddiqui, S.A. 2024. Effect of supplementation of papaya seed (Carica papaya) on growth performance, carcass traits, and histomorphology of Japanese quails. Pakistan Journal of Zoology. pp 1-4. https://dx.doi.org/10.17582/journal.pjz/20230824082655.

Salmonella infection in poultry: A review on the pathogen and control strategies - (Review Article)
Syamily, S., Selvaraj, R., Shanmugasundaram, R. 2023. Salmonella infection in poultry: A review on the pathogen and control strategies. Microorganisms. 11(11), p. 2814. https://doi.org/10.3390/microorganisms11112814.

Estimation of the antibacterial and anti-tumor impacts of soy milk and ecofriendly myco-manufactured zinc oxide nanomaterials - (Peer Reviewed Journal)
Helmy, E.A., Amin, B.H., Alqhtani, A.H., Pokoo-Aikins, A., Yosri, M. 2024. Estimation of the antibacterial and anti-tumor impacts of soy milk and ecofriendly myco-manufactured zinc oxide nanomaterials. Polish Journal of Environmental Studies. 33(3):2093-2102. https://doi.org/10.15244/pjoes/174792.

Synergistic effects of essential oils and organic acids against aspergillus flavus contamination in poultry feed - (Peer Reviewed Journal)
Satterlee, T.R., Mcdonough, C.M., Gold, S.E., Chen, C., Glenn, A.E., Pokoo-Aikins, A. 2023. Synergistic effects of essential oils and organic acids against aspergillus flavus contamination in poultry feed. Toxins. 15(11), p.635. https://doi.org/10.3390/toxins15110635.

Comparison of floored and cage housed broiler breeder farms for energy and economic efficiency in Pakistan: A case study of broiler breeder's farm in district Punjab - (Peer Reviewed Journal)
Khan, I., Khan, S., Shuaib, M., Hassan, U., Alqhtani, A.H., Pokoo-Aikins, A., Qureshi, A., Ali, M., Alam, W. 2024. Comparison of floored and cage housed broiler breeder farms for energy and economic efficiency in Pakistan: A case study of broiler breeder's farm in district Punjab. Pakistan Journal of Zoology. pp.1-11. https://doi.org/10.17582/journal.pjz/20230810114347.

Effect of dietary xylo-oligosaccharide on growth performance, intestinal histomorphology, and specific cecal bacteria in broiler chickens - (Peer Reviewed Journal)
Yadav, S., Singh, A.K., Selvaraj, R., Applegate, T., Bhattacharya, P., Shinall, S.B., Fenn, L., Shanmugasundaram, R., Kim, W. 2023. Effect of dietary xylo-oligosaccharide on growth performance, intestinal histomorphology, and specific cecal bacteria in broiler chickens. Poultry Science. 103(1), 103189.. https://doi.org/10.1016/j.psj.2023.103189.

Mycotoxin contamination and nutritional content of corn - (Abstract Only)

Investigation of the Impact of Mycogenic Titanium and Selenium Nanoparticles on Fusarium Wilt Infection of Tomato Plant - (Peer Reviewed Journal)
Helmy, E.A., Salah, R.A., El-Shazly, M.M., Alqhtani, A.H., Pokoo-Aikins, A., Mohammed, Y. 2023. Investigation of the Impact of Mycogenic Titanium and Selenium Nanoparticles on Fusarium Wilt Infection of Tomato Plant. Journal of Pure and Applied Microbiology. 2023;17(3):1800-1813. https://doi.org/10.22207/JPAM.17.3.45.

Effect of synbiotic supplementation on production performance and severity of necrotic enteritis in broilers during an experimental necrotic enteritis challenge - (Peer Reviewed Journal)
Shah, B., Hakeem, W., Shanmugasundaram, R., Selvaraj, R. 2023. Effect of synbiotic supplementation on production performance and severity of necrotic enteritis in broilers during an experimental necrotic enteritis challenge. Poultry Science. 102959; 1-23. https://doi.org/10.1016/j.psj.2023.102959.

Growth performance, intestinal morphometry, and blood serum parameters of broiler chickens fed diets containing increasing levels of wheat bran with or without exogenous multi-enzyme supplementation - (Peer Reviewed Journal)
Idan, F., Paulk, C.B., Pokoo-Aikins, A., Stark, C. 2023. Growth performance, intestinal morphometry, and blood serum parameters of broiler chickens fed diets containing increasing levels of wheat bran with or without exogenous multi-enzyme supplementation. Livestock Science. 275 (2023) 105296. https://doi.org/10.1016/j.livsci.2023.105296.

Efficacy and optimal feeding level of rumex nervosus leaves on bood bochemistry, carcass characteristics, productivity indices, and anticoccidial indicators of broiler chickens infected or not infected with eimeria tenella - (Peer Reviewed Journal)
Alqhtani, A.H., Qaid, M.M., Al-Mufarrej, S.I., Al-Garadi, M.A., Pokoo-Aikins, A. 2023. Efficacy and optimal feeding level of rumex nervosus leaves on bood bochemistry, carcass characteristics, productivity indices, and anticoccidial indicators of broiler chickens infected or not infected with eimeria tenella. Brazilian Journal of Poultry Science. 25,2022-1786. https://doi.org/10.1590/1806-9061-2022-1786.

Editorial: Perspectives in avian skeletal systems and skeletal abnormalities - (Review Article)
Chen, C., Pokoo-Aikins, A., Huang, S., Regmi, P., Rehman, M. 2023. Editorial: Perspectives in avian skeletal systems and skeletal abnormalities. Frontiers in Physiology. 14:1229943. https://doi.org/10.3389/fphys.2023.1229943.

Bathing in Oils and Acids: how combining natural antimicrobials demonstrate synergy in combating Aflatoxin contamination - (Abstract Only)

Effect of killed salmonella bacterin vaccines on immunological response and hen day egg production in layers. - (Abstract Only)

Effect of synbiotic supplementation on production performance and intestinal inflammatory response in broilers under induced necrotic enteritis challenge - (Abstract Only)

Comparing the effect of synbiotic and antibiotic supplementation on production performance and necrotic enteritis severity in broilers under an experimental necrotic enteritis challenge - (Abstract Only)

Dose-response effects of combined fumonisins, deoxynivalenol, and zearalenone mycotoxins on the 1-alpha hydroxylase m-RNA expression and blood biochemistry - (Abstract Only)

The effects of arginine and branched-chain amino acid supplementation to reduced-protein diet on intestinal health, cecal short-chain fatty acid profiles, and immune response in broiler chickens challenged with Eimeria spp. - (Peer Reviewed Journal)
Liu, G., Ajao, A.M., Shanmugasundaram, R., Taylor, J., Ball, E., Applegate, T.J., Selvaraj, R., Kyriazakis, I., Olukosi, O.A., Kim, W.K. 2023. The effects of arginine and branched-chain amino acid supplementation to reduced-protein diet on intestinal health, cecal short-chain fatty acid profiles, and immune response in broiler chickens challenged with Eimeria spp.. Poultry Science. 102 (7): 1-17. https://doi.org/10.1016/j.psj.2023.102773.

Dose-response effects of combined doses of fumonisins, deoxynivalenol, and zearalenone mycotoxins on major T-cell subsets and tight junction protein expressions in broiler chickens - (Abstract Only)

Short-term exposure to fumonisins and deoxynivalenol, on broiler growth performance and cecal Salmonella load during experimental Salmonella enteritidis infection - (Peer Reviewed Journal)
Liu, J., Shanmugasundaram, R., Doupovec, B., Dian, S., Ganapathi, R., Applegate, T. 2023. Short-term exposure to fumonisins and deoxynivalenol, on broiler growth performance and cecal Salmonella load during experimental Salmonella enteritidis infection. Poultry Science. 102(6):102677. https://doi.org/10.1016/j.psj.2023.102677.

Subclinical doses of dietary fumonisins and deoxynivalenol causes cecal microbiota dysbiosis in broiler chickens challenged with clostridium perfringens - (Peer Reviewed Journal)
Shanmugasundaram, R., Lourenco, J., Hakeem, W.A., Dycus, M., Applegate, T. 2023. Subclinical doses of dietary fumonisins and deoxynivalenol causes cecal microbiota dysbiosis in broiler chickens challenged with clostridium perfringens. Frontiers in Microbiology. 14:1-14. https://doi.org/10.3389/fmicb.2023.1106604.

Yeasts and yeast-based products in poultry nutrition - (Review Article)
Fathima, S., Shanmugasundaram, R., Sifri, M., Selvaraj, R. 2023. Yeasts and yeast-based products in poultry nutrition. Journal of Applied Poultry Research. 32(2):100345. https://doi.org/10.1016/j.japr.2023.100345.

The detection and co-occurrence of mycotoxins in feed corn using HPLC/MS analysis - (Abstract Only)

Effect of combined doses of fumonisin, deoxynivalenol and zearalenone on production performance and amino acid digestibility in broiler chickens - (Abstract Only)

Effects of synbiotic supplementation as an antibiotic growth promoter replacement on cecal Campylobacter jejuni load in broilers challenged with C. jejuni - (Peer Reviewed Journal)
Cason, E.E., Al Hakeem, W., Adams, D., Shanmugasundaram, R., Selvaraj, R. 2022. Effects of synbiotic supplementation as an antibiotic growth promoter replacement on cecal Campylobacter jejuni load in broilers challenged with C. jejuni. Journal of Applied Poultry Research. 32(2):100315. https://doi.org/10.1016/j.japr.2022.100315.

In vitro antibacterial and antifungal efficacy assessment of ethanolic and aqueous extracts of Rumex nervosus leaves against selected bacteria and fungi - (Peer Reviewed Journal)
Al-Garadi, A., Quid-Mohammed, M., Alqhtani-Abdulmohsen, H., Pokoo-Aikins, A., Al-Mufarrej-Saud, I. 2022. In vitro antibacterial and antifungal efficacy assessment of ethanolic and aqueous extracts of Rumex nervosus leaves against selected bacteria and fungi. Veterinary world. 15(11):2725-2737. https://doi.org/10.14202/vetworld.2022.2725-2737.

Campylobacter jejuni in poultry: pathogenesis and control strategies - (Review Article)
Hakeem, A., Fathima, S., Selvaraj, R., Shanmugasundaram, R. 2022. Campylobacter jejuni in poultry: pathogenesis and control strategies. Microorganisms. 10(11):2134. https://doi.org/10.3390/microorganisms10112134.

Necrotic enteritis in broiler chickens: A review on the pathogen, pathogenesis, and prevention - (Review Article)
Fathima, S., Hakeem, A., Shanmugasundaram, R., Selvaraj, R. 2022. Necrotic enteritis in broiler chickens: A review on the pathogen, pathogenesis, and prevention. Microorganisms. 10(10):1958. https://doi.org/10.3390/microorganisms10101958.

Fusarium verticillioides NAT1 (FDB2) N-malonyltransferase is structurally, functionally, and phylogenetically distinct from its N-acetyltransferase (NAT) homologues - (Peer Reviewed Journal)
Karagianni, E., Kontomina, E., Lowe, E., Athanasopoulos, K., Papanikolaou, G., Garefalaki, V., Kotseli, V., Zaliou, S., Grimaud, T., Arvaniti, K., Tsatiri, M., Fakis, G., Glenn, A.E., Roversi, P., Abuhammad, A., Ryan, A., Sim, R., Sim, E., Boukouvala, S. 2022. Fusarium verticillioides NAT1 (FDB2) N-malonyltransferase is structurally, functionally, and phylogenetically distinct from its N-acetyltransferase (NAT) homologues. FEBS Journal. 290:2412-2436. https://doi.org/10.1111/febs.16642.

Subclinical Doses of Dietary Fumonisins and Deoxynivalenol Alter the Cecal Microbiota of Broiler Chickens - (Abstract Only)

Subclinical doses of combined fumonisins and deoxynivalenol predispose Clostridium perfringens-inoculated broilers to necrotic enteritis - (Abstract Only)

The incredible edible egg: dried egg product improves broiler growth performance and supplements cocci vaccines in Eimeria and necrotic enteritis challenged birds - (Abstract Only)

Immune responses, gut permeability, and cecal Campylobacter jejuni loads in an experimental C. jejuni challenge model in broilers - (Abstract Only)

Effects of synbiotic supplementation as an antibiotic growth promoter replacement on cecal Campylobacter jejuni load in broilers challenged with C. jejuni - (Abstract Only)

Determination of therapeutic and safety effects of zygophyllum coccineum extract in induced inflammation in rats - (Peer Reviewed Journal)
Yosri, M., Elaasser, M.M., Abdel-Aziz, M.M., Hassan, M.M., Alqhtani, A.H., Al-Gabri, N., Ali, A.B., Pokoo-Aikins, A., Amin, B.H. 2022. Determination of therapeutic and safety effects of zygophyllum coccineum extract in induced inflammation in rats. BioMed Research International. 2022. Article 7513155. https://doi.org/10.1155/2022/7513155.

Subclinical doses of combined fumonisins and deoxynivalenol predispose Clostridium perfringens-inoculated broilers to necrotic enteritis - (Peer Reviewed Journal)
Shanmugasundaram, R., Adams, D., Ramirez, S., Murugesan, R., Applegate, T., Cunningham, S.B., Pokoo-Aikins, A., Glenn, A.E. 2022. Subclinical doses of combined fumonisins and deoxynivalenol predispose Clostridium perfringens-inoculated broilers to necrotic enteritis. Frontiers in Physiology. 13: 1-15. https://dx.doi.org/10.3389/fphys.2022.934660.

Combining maternal and post-hatch dietary 25-hydroxycholecalciferol supplementation on broiler chicken growth performance and carcass characteristics - (Peer Reviewed Journal)
Avila, L.P., Leiva, S.F., Abascal-Ponciano, G.A., Flees, J.J., Sweeney, K.M., Wilson, J.L., Turner, B., Litta, G.A., Waguespack-Levy, A.M., Pokoo-Aikins, A., Starkey, G.W. 2022. Combining maternal and post-hatch dietary 25-hydroxycholecalciferol supplementation on broiler chicken growth performance and carcass characteristics. Poultry. 1(2):111–124. https://doi.org/10.3390/poultry1020010.